Exam 17: Bacterial and Viral Genetics

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The core of a virus is a ____ molecule.

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Arrange the following steps of the HIV life cycle in order: 1.Viral RNAs and proteins assemble into new viral particles that bud from the cell. 2)DNA copy of the viral genome is integrated into the host genome. 3)Viral reverse transcriptase makes a double-stranded DNA copy of the viral RNA genome. 4)Viral particle enters the cell. 5)The provirus is transcribed into viral RNA genomes and mRNAs to make viral proteins.

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  Figure 17.2 - The accompanying figure above shows the distances between genes in minutes. Assume that the time listed between two genes corresponds to the transfer time of the first of the two genes in the pair. Use the figure to answer questions . Suppose that after bacterial conjugation, the recipient cell contained the a and b genes. From this information, what can you conclude? Figure 17.2 - The accompanying figure above shows the distances between genes in minutes. Assume that the time listed between two genes corresponds to the transfer time of the first of the two genes in the pair. Use the figure to answer questions . Suppose that after bacterial conjugation, the recipient cell contained the a and b genes. From this information, what can you conclude?

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  Figure 17.2 - The accompanying figure above shows the distances between genes in minutes. Assume that the time listed between two genes corresponds to the transfer time of the first of the two genes in the pair. Use the figure to answer questions . According to the accompanying figure, what is the order in which the genes will be transferred to an F- cell during conjugation? Figure 17.2 - The accompanying figure above shows the distances between genes in minutes. Assume that the time listed between two genes corresponds to the transfer time of the first of the two genes in the pair. Use the figure to answer questions . According to the accompanying figure, what is the order in which the genes will be transferred to an F- cell during conjugation?

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How does a phage transfer DNA from one bacterium to another?

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Which statement correctly describes horizontal gene transfer?

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Match each of the following terms with its correct definition.
Lytic cycle
Type of virus that kills a host cell in every replication cycle
Reverse transcriptase
Type of virus that does not kill a host cell in every replication cycle
Latent stage
E. coli phage.
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Lytic cycle
Type of virus that kills a host cell in every replication cycle
Reverse transcriptase
Type of virus that does not kill a host cell in every replication cycle
Latent stage
E. coli phage.
Transduction
Virus with an RNA genome that is copied into DNA.
Conjugation
During this process, DNA is transferred from one cell to another through a cytoplasmic bridge.
Capsid
Enzyme that can make DNA from RNA.
T-even bacteriophages
Includes phages such as T2 and T4.
Bacteriophage lambda
The cycle in which a virulent phage kills the bacterial cell by lysis as the phage is released.
Temperate phage
Layer of protein surrounding the viral nucleic acid.
Retrovirus
This cycle begins when the lambda phage chromosome integrates into the host cell's DNA by crossing-over. Once integrated, the lambda genes are mostly inactive.
Prophage
When an animal virus remains in the cell in an inactive form.
Virulent phage
Integrated viral DNA.
Lysogenic cycle
Transferring DNA using a virus.
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  Figure 17.2 - The accompanying figure above shows the distances between genes in minutes. Assume that the time listed between two genes corresponds to the transfer time of the first of the two genes in the pair. Use the figure to answer questions. In order to transfer gene a to an F<sup>-</sup>cell, the sex pilus must be in place for at least ____ minutes. Figure 17.2 - The accompanying figure above shows the distances between genes in minutes. Assume that the time listed between two genes corresponds to the transfer time of the first of the two genes in the pair. Use the figure to answer questions. In order to transfer gene a to an F-cell, the sex pilus must be in place for at least ____ minutes.

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During bacterial conjugation, bacteria use a long tubular structure called a(n) ____ to contact each other.

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  Figure 17.1 Use the accompanying figure . If strains 1 and 2 conjugate and are plated on minimal medium, cells growing on the minimal medium will have the genotype ____. Figure 17.1 Use the accompanying figure . If strains 1 and 2 conjugate and are plated on minimal medium, cells growing on the minimal medium will have the genotype ____.

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Hfr cells are bacterial cells that ____.

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The results of Lederberg and Tatum's experiments with E. coli auxotrophs indicated that ____.

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The series of events from infection of a cell through the release of progeny phages from a lysed cell is called the ____ cycle.

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Prions ____.

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Bacteria that undergo natural transformation ____.

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T-even bacteriophages ____.

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The purpose of the sex pilus is to ____.

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How do the constituents of liquid medium differ from solid, gel-like medium?

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Conjugation, transduction, and transformation are the three main mechanisms for gene transfer between bacteria. Compare these three mechanisms in terms of how DNA is transferred.

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A colony of mice is treated with a nonvirulent strain of the bacterium Streptococcus pneumoniae . Before treatment, a researcher exposed this nonvirulent strain to heat-killed cells of a virulent strain. What will be the effect on the mouse population?

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