Exam 10: Genetics of Bacteria and Arachaea

Discuss the similarities and differences between transposons and insertion sequence (IS) elements.

When the F factor is integrated, all Hfr strains have the origin of replication functioning in the same direction.

The IS element cannot code for the transposase enzyme.

A plasmid may

A mutant that has a nutritional requirement for growth is an example of a(n)

Which is a phenotype of an F- cell?

Hfr strains of Escherichia coli

The F plasmid of Escherichia coli is about 99 kbp and is present in both F+ and F- strains.

A bacterium containing two copies of a chromosomal nucleotide sequence is called a(n)________. Typically one copy is present on the _________ and the other on a(n)________ .

The enzyme transposase may be coded for by (the)

Bacterial mating (or conjugation) is a bidirectional process where nucleic acids (DNA or RNA) are transferred during a cell- to- cell interaction involving a donor and a recipient cell.

A typical mutation rate for a bacterium is in the range of 10- 6 to 10- 9 per kbp.

Plasmids that govern their own transfer are known as

In specialized transduction, as exemplified in the lambda- Gal system, transduction by a lysate occurs at high efficiency for only a restricted group of gal genes, and induction of a lysogenic cell is required.

Intercalating agents, like acridine orange and ethidium bromide, lead to mutagenesis by pushing DNA base pairs apart, which can lead to insertions or deletions.

A mutation that readily reverses to restore the original parental type would most likely be due to a(n)

Almost all plasmids are double- stranded DNA.

Which of the following would NOT be a trait of a resistance plasmid?

A mutation in recA results in

The two primary mechanisms of transposition are _________ and________ .