Exam 22: Methods in Microbial Ecology

What unit is isotopic decay reported as?

Describe what an ITS region is in the context of prokaryotes, and explain the significance of it being studied in microbial communities.

Analyses of __________ isotopes have been used as evidence against life on the Moon.

The genus of the most abundant phototrophic organism on Earth is_________ .

What prior knowledge is required to use a phylochip to study the abundances of the microbes present within a microbial community?

Collective estimates of the physiological reactions occurring in the entire microbial community are known as __________ measurements.

In natural samples it is often difficult to differentiate live cells from dead cells or cells from nonliving matter.

As a microbial examination technique, FISH stands for __________ .

An artificial microbial ecosystem that serves as a long- term source of bacteria for enrichment cultures is called a(n)__________ .

For what purpose would a microbial ecologist use a microelectrode?

Radioisotopes are useful when

The overall purpose of DGGE is to__________ .

The rolled tube method is most commonly used for isolating which grouping of microorganisms?

Phylogenetic stains, such as those used in FISH, hybridize with

Metatranscriptomics and metaproteomics involve studying the total community of__________and__________ , respectively. These are favored over metagenomics when__________is of more interest than gene presence.

If properly constructed, a Winogradsky column can be used to isolate pure cultures.

Culture independent approaches circumvent issues associated with_________.

The biodiversity metric depends on both __________and __________of organisms present within a community.

Two __________ are hybridized to a complementary sequence in a target gene in PCR technology.

Which method requires the use of at least one radioactive chemical?