Exam 6: Protein Function

Complete the following bacterial ABC transporter protein process: 1. Binding of substrate carrier protein induces a conformational change to expose the substrate binding site. 2. ATP hydrolysis causes a conformational change that releases substrate. 3.

Which of the following is a positive effector for oxygen binding to hemoglobin?

The translocation rate of a solute across a membrane is measured. A plot of translocation rate versus solute concentration displays a hyperbolic curve. The translation

Gramicidin A is involved in the __________ transport of __________ charged ions.

The figure below shows the oxygen binding curves of oxygen-transport protein from four different organisms. Decide which organism is the most efficient at oxygen transport and justify your choice.

A myoblast contain(s) __________, but a myofibril does not.

Which of the following groups are hydrogen bonded in oxyhemoglobin but not deoxyhemoglobin?

Oxygen binding is monitored for a solution of hemoglobin. During the experiment, the curve changes from sigmoidal to hyperbolic. Which of the following may be the reason for the change?

The estrogen receptor is a(n)

A reduction in the catalytic ability of carbonic anhydrase may lead to efficient oxygen delivery by hemoglobin as a result of a(n) Bohr effect.

Which of the following may result from a His143 $\rightarrow$ Ala143 mutation in adult hemoglobin?

A protein that follows the concerted model of allosteric regulation would display a binding curve that is . A protein that follows the sequential model of allosteric regulation would display a binding curve that is .

Using the oxygen binding curve shown, calculate the approximate percentage of O₂ that is released from hemoglobin as the pO₂ changes from 13 kPa to 4 kPa.

Articulate the difference between a myoblast and a myofibril.

Explain how the five arginine residues found within the channel of Omp32 protein of the bacterium Delftia acidovorans increase the specificity of the channel.

Which of the following is NOT a definition of fractional saturation for a system of [P] +[L] $\harr$ [PL]?

Radioactive iodine can be used in laboratory settings to label inhibitors. When working in a lab, a researcher is exposed to the radioactive iodine when some accidentally spills on her exposed wrist. A scan with a Geiger counter the next day indicates that most of the radioactivity is located in her neck. Predict how this localization occurred and include any transporters that may be involved.

An oxygen binding curve of fractional saturation versus pO₂ displays a sigmoidal shape. This is the oxygen binding curve for

Both SERCA and the Na⁺-K⁺ ATPase contain a P domain. Explain the general function of this domain.

Using the following data, arrange the proteins in order from the one having the lowest affinity for the ligand to the highest.