Question 1

As 18 Na+ ions are exported out of the cell by the Na+-K+ ATPase membrane transport protein, K+ ions are imported into the cell.

Accepted Answer

A)  2 
B)  12 
C)  18 
D)  24 
A)  2 
B)  12 
C)  18 
D)  24

Question 2

The mutation that would lead to the most dramatic effect on Ca2+ transport is a mutation of

Accepted Answer

A)  Ser16 in phospholamban. 
B)  Thr17 in phospholamban. 
C)  Asp351 in SERCA. 
D)  Ser17 in E2P. 
A)  Ser16 in phospholamban. 
B)  Thr17 in phospholamban. 
C)  Asp351 in SERCA. 
D)  Ser17 in E2P.

Question 3

Given the following data for myoglobin and O2 concentrations, what is $\theta$ ?
[Mb] = 0.07 mM, [MbO2] = 0.69 mM, [O2] = 3.5 kPa.

Accepted Answer

The answer of Given the following data for myoglobin and...

Question 4

Decide if the rate of malate transport by the Omp32 protein of Delftia acidovorans will be affected if the pH of the system is changed from 6.8 to 8.2. Explain your reasoning.
The pKa of amino acids with ionizable side chains are provided in the table below. Note that malic acid contains two ionizable groups with pKa of 3.40 and 5.20. Assume in this scenario that the pH change does not alter the overall conformation of the protein.

Accepted Answer

The rate will not be changed s

Question 5

Below is a figure of adult hemoglobin. The arrow points to where binds.

Accepted Answer

A)  glucose 
B)  2,3-bisphosphoglycerate 
C)  O2 
D)  CO2 
A)  glucose 
B)  2,3-bisphosphoglycerate 
C)  O2 
D)  CO2

Question 6

As K+ passes through the selectivity filter of the K+ channel of Streptomyces lividans it interacts with

Accepted Answer

A)  glycine side chains. 
B)  amino acid carbonyl groups. 
C)  tyrosine side chains. 
D)  amino acid amino groups. 
A)  glycine side chains. 
B)  amino acid carbonyl groups. 
C)  tyrosine side chains. 
D)  amino acid amino groups.

Question 7

Approximately how many amino acids are identical in both myoglobin and the adult $\alpha$-subunit of hemoglobin?

Accepted Answer

A)  2% 
B)  20% 
C)  38% 
D)  81% 
A)  2% 
B)  20% 
C)  38% 
D)  81%

Question 8

Which of the following would shift the oxygen binding curve for hemoglobin to the left?

Accepted Answer

A)  a lower concentration of CO2 
B)  a higher pH 
C)  a higher concentration of 2,3-BPG 
D)  an increase in the number of hemoglobin molecules in the T state 
A)  a lower concentration of CO2 
B)  a higher pH 
C)  a higher concentration of 2,3-BPG 
D)  an increase in the number of hemoglobin molecules in the T state

Question 9

Consider the movement of fructose into a cell by passive transport. If the extracellular concentration of fructose is 0.35 mM and the intracellular concentration of fructose is
0.42 mM, what is the free energy of transport in kJ/mol?

Accepted Answer

The answer of Consider the movement of fructose into a...

Question 10

What is the clinical term for reduced O2 transport efficiency from the lungs to the tissues, arising from altered hemoglobin function or reduced numbers of red blood cells per unit of blood? Provide an example of a specific type of anemia that is caused by a single point
mutation.

Accepted Answer

Anemia; si

Question 11

You design an experiment in which you can monitor the step-by-step movement of H+ and Ca2+ through the SERCA protein, starting with the protein in the E2-ATP state and moving through the transport cycle, ultimately returning to the E2-ATP state. Your control experiment includes everything required for the system to function normally, and you easily detect four distinct conformations of the protein. You then replace the ATP in the system with ATP$\gamma$S, a nonhydrolyzable form of ATP, and begin the experiment again with the
protein in the E2-ATP state. Predict which conformations of SERCA you will detect.

Accepted Answer

Only the E2-ATP conformation will be det

Question 12

Which of the following is a pharmaceutical drug that targets an active transporter?

Accepted Answer

A)  sertraline 
B)  malate 
C)  gramicidin A 
D)  2,3-BPG 
A)  sertraline 
B)  malate 
C)  gramicidin A 
D)  2,3-BPG

Question 13

Consider the following interaction between a protein and its ligand: [P] + [L]   $\harr$ [PL]. If [L] = 5 mM, [P] = 3 mM, and [PL] = 2 mM, what is the Ka for the interaction?

Accepted Answer

A)  7.5 mM 
B)  15 mM 
C)  0.133 mM 
D)  0.4 mM 
A)  7.5 mM 
B)  15 mM 
C)  0.133 mM 
D)  0.4 mM

Question 14

A cell with a membrane potential of -70 mV that contains functioning Na+-K+ ATPase membrane transport proteins will maintain an extracellular __________ concentration that is __________ than the intracellular concentration.

Accepted Answer

A)  Na+; lower; K+ 
B)  Na+; lower; Na+ 
C)  K+; higher; Na+ 
D)  K+; lower; K+ 
A)  Na+; lower; K+ 
B)  Na+; lower; Na+ 
C)  K+; higher; Na+ 
D)  K+; lower; K+

Question 15

When oxygen binds to hemoglobin, which of the following occurs?

Accepted Answer

A)  The heme group becomes puckered. 
B)  The atomic radius of the iron is decreased. 
C)  His F7 is brought closer to the plane of the porphyrin ring. 
D)  His E8 is brought closer to the plane of the porphyrin ring. 
A)  The heme group becomes puckered. 
B)  The atomic radius of the iron is decreased. 
C)  His F7 is brought closer to the plane of the porphyrin ring. 
D)  His E8 is brought closer to the plane of the porphyrin ring.

Question 16

What type of transport is depicted in the figure below?

Accepted Answer

A)  membrane diffusion 
B)  passive transport of molecules down a concentration gradient 
C)  active transport of molecules down a concentration gradient 
D)  active transport of molecules up a concentration gradient 
A)  membrane diffusion 
B)  passive transport of molecules down a concentration gradient 
C)  active transport of molecules down a concentration gradient 
D)  active transport of molecules up a concentration gradient

Question 17

Consider the movement of glucose into a cell by passive transport. If the extracellular concentration of glucose is 4 mM and the intracellular concentration of glucose is 2 mM, what is the free energy of transport in kJ/mol?

Accepted Answer

The answer of Consider the movement of glucose into a...

Question 18

Which of the following statements is true for hemoglobin but NOT myoglobin?

Accepted Answer

A)  The tertiary structure is made up of a globin fold. 
B)  The surface of its tertiary structure contains many hydrophobic amino acids. 
C)  It contains a proximal histidine that coordinates the heme group. 
D)  It contains a single carboxyl-terminal amino acid. 
A)  The tertiary structure is made up of a globin fold. 
B)  The surface of its tertiary structure contains many hydrophobic amino acids. 
C)  It contains a proximal histidine that coordinates the heme group. 
D)  It contains a single carboxyl-terminal amino acid.

Question 19

Consider the transport scenario shown. Which curve represents the overall movement of the small molecule from the extracellular environment to the cytosol?

Accepted Answer

A)    
B)    
C)    
D)    
A)    
B)    
C)    
D)

Question 20

In the shift from the T to the R state in adult hemoglobin, a shift between the subunits occurs. Explain how this shift leads to the variation of binding affinity for 2,3-BPG in the two states.

Accepted Answer

The shift from the T state to the R stat

Exam 6: Protein Function

As 18 Na+ ions are exported out of the cell by the Na+-K+ ATPase membrane transport protein, K+ ions are imported into the cell.

The mutation that would lead to the most dramatic effect on Ca2+ transport is a mutation of

Given the following data for myoglobin and O2 concentrations, what is θ\thetaθ ? [Mb] = 0.07 mM, [MbO2] = 0.69 mM, [O2] = 3.5 kPa.

Below is a figure of adult hemoglobin. The arrow points to where binds.

As K+ passes through the selectivity filter of the K+ channel of Streptomyces lividans it interacts with

Approximately how many amino acids are identical in both myoglobin and the adult α\alphaα -subunit of hemoglobin?

Which of the following would shift the oxygen binding curve for hemoglobin to the left?

Consider the movement of fructose into a cell by passive transport. If the extracellular concentration of fructose is 0.35 mM and the intracellular concentration of fructose is 0.42 mM, what is the free energy of transport in kJ/mol?

What is the clinical term for reduced O2 transport efficiency from the lungs to the tissues, arising from altered hemoglobin function or reduced numbers of red blood cells per unit of blood? Provide an example of a specific type of anemia that is caused by a single point mutation.

Which of the following is a pharmaceutical drug that targets an active transporter?

Consider the following interaction between a protein and its ligand: [P] + [L] ↔\harr↔ [PL]. If [L] = 5 mM, [P] = 3 mM, and [PL] = 2 mM, what is the Ka for the interaction?

A cell with a membrane potential of -70 mV that contains functioning Na+-K+ ATPase membrane transport proteins will maintain an extracellular __________ concentration that is __________ than the intracellular concentration.

When oxygen binds to hemoglobin, which of the following occurs?

What type of transport is depicted in the figure below?

Consider the movement of glucose into a cell by passive transport. If the extracellular concentration of glucose is 4 mM and the intracellular concentration of glucose is 2 mM, what is the free energy of transport in kJ/mol?

Which of the following statements is true for hemoglobin but NOT myoglobin?

Consider the transport scenario shown. Which curve represents the overall movement of the small molecule from the extracellular environment to the cytosol?

In the shift from the T to the R state in adult hemoglobin, a shift between the subunits occurs. Explain how this shift leads to the variation of binding affinity for 2,3-BPG in the two states.

Principles of Biochemistry

Physical Biochemistry: Energy Conversion, Water, and Membranes

Nucleic Acid Structure and Function

Protein Structure

Methods in Protein Biochemistry

Enzyme Mechanisms

Cell Signaling Systems

Glycolysis: a Paradigm of Metabolic Regulation

The Citrate Cycle

Oxidative Phosphorylation

Photosynthesis

Carbohydrate Structure and Function

Carbohydrate Metabolism

Lipid Structure and Function

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Metabolic Integration

DNA Replication, Repair, and Recombination

RNA Synthesis, Processing, and Gene Silencing

Protein Synthesis, Posttranslational Modification, and Transport

Gene Regulation

Filters

As 18 Na⁺ions are exported out of the cell by the Na⁺-K⁺ATPase membrane transport protein, K⁺ions are imported into the cell.

The mutation that would lead to the most dramatic effect on Ca²⁺transport is a mutation of

Given the following data for myoglobin and O₂ concentrations, what is $\theta$ ? [Mb] = 0.07 mM, [MbO₂] = 0.69 mM, [O₂] = 3.5 kPa.

As K⁺passes through the selectivity filter of the K⁺channel of Streptomyces lividans it interacts with

Approximately how many amino acids are identical in both myoglobin and the adult $\alpha$ -subunit of hemoglobin?

What is the clinical term for reduced O₂ transport efficiency from the lungs to the tissues, arising from altered hemoglobin function or reduced numbers of red blood cells per unit of blood? Provide an example of a specific type of anemia that is caused by a single point mutation.

Consider the following interaction between a protein and its ligand: [P] + [L] $\harr$ [PL]. If [L] = 5 mM, [P] = 3 mM, and [PL] = 2 mM, what is the Ka for the interaction?

A cell with a membrane potential of -70 mV that contains functioning Na⁺-K⁺ATPase membrane transport proteins will maintain an extracellular concentration that is than the intracellular concentration.