Question 1

How is transcription factor recruitment used in eukaryotic gene regulatory circuits?

Accepted Answer

Recruitment refers to the property of tr

Question 2

When IPTG is added to a cell, what is the predicted outcome?

Accepted Answer

A)  Protein synthesis stops. 
B)  Protein overproduction occurs. 
C)  The DNA sequence coding for a given protein is altered. 
D)  An epigenetic state occurs. 
A)  Protein synthesis stops. 
B)  Protein overproduction occurs. 
C)  The DNA sequence coding for a given protein is altered. 
D)  An epigenetic state occurs.

Question 3

IPTG causes the lac promoter to

Accepted Answer

A)  bind irreversibly to the DNA. 
B)  dissociate from the DNA. 
C)  perform ligand-regulated repression. 
D)  perform ligand-activated deregulation. 
A)  bind irreversibly to the DNA. 
B)  dissociate from the DNA. 
C)  perform ligand-regulated repression. 
D)  perform ligand-activated deregulation.

Question 4

When Cro is bound to OR3, the

Accepted Answer

A)  lysogenic pathway is favored. 
B)  lytic pathway is favored. 
C)  Cro protein inhibits the PR promoter. 
D)  CI protein activates the PRM promoter. 
A)  lysogenic pathway is favored. 
B)  lytic pathway is favored. 
C)  Cro protein inhibits the PR promoter. 
D)  CI protein activates the PRM promoter.

Question 5

What are the two main approaches being considered for treatment using iPS cell technology?

Accepted Answer

The first approach is to reintroduce cel

Question 6

When there is no lactose in a cell, production of lac operon

Accepted Answer

A)  increases. 
B)  decreases. 
C)  turns off completely. 
D)  production of lac operon does not depend on lactose concentration. 
A)  increases. 
B)  decreases. 
C)  turns off completely. 
D)  production of lac operon does not depend on lactose concentration.

Question 7

Histone acetylation leads to transcriptional activation by

Accepted Answer

A)  increasing the interaction between the histone tail and DNA. 
B)  decreasing the interaction between the histone tail and DNA. 
C)  strengthening the interactions between nucleosomes. 
D)  removing binding sites for additional transcriptional factors. 
A)  increasing the interaction between the histone tail and DNA. 
B)  decreasing the interaction between the histone tail and DNA. 
C)  strengthening the interactions between nucleosomes. 
D)  removing binding sites for additional transcriptional factors.

Question 8

The best description of how allosteric regulation works is that a ligand the transcriptional regulatory protein, which __________ the protein's affinity for DNA.

Accepted Answer

A)  covalently modifies; increases 
B)  covalently modifies; decreases 
C)  binds to; causes conformational changes that affect 
D)  binds to; causes conformational changes that can only decrease 
A)  covalently modifies; increases 
B)  covalently modifies; decreases 
C)  binds to; causes conformational changes that affect 
D)  binds to; causes conformational changes that can only decrease

Question 9

Using the figure below, explain how cooperativity works.

Accepted Answer

Weak interactions between the three comp

Question 10

The Swi-Snf complex affects chromatin by

Accepted Answer

A)  creating extra nucleosomes near the promoter. 
B)  being a promoter. 
C)  creating a nucleosome-free region near the promoter. 
D)  transcribing the chromatin. 
A)  creating extra nucleosomes near the promoter. 
B)  being a promoter. 
C)  creating a nucleosome-free region near the promoter. 
D)  transcribing the chromatin.

Question 11

The function of RecA* is to

Accepted Answer

A)  inhibit expression of its own gene. 
B)  repress SOS genes. 
C)  stimulate LexA autocleavage. 
D)  allow LexA to accumulate. 
A)  inhibit expression of its own gene. 
B)  repress SOS genes. 
C)  stimulate LexA autocleavage. 
D)  allow LexA to accumulate.

Question 12

Describe the three biochemical consequences of histone acetylation.

Accepted Answer

First, it weakens the interaction betwee

Question 13

Which of the following best describes how trans- and cis-acting factors operate?

Accepted Answer

A)  Cis-acting factors can bind to specific DNA sequences whereas trans-acting sites are DNA sequences. 
B)  Trans-acting factors can bind to specific DNA sequences whereas cis-acting sites are DNA sequences. 
C)  Trans- and cis-acting factors can both bind to specific DNA sequences. 
D)  Trans- and cis-acting factors can both only bind to DNA elements to which they are physically linked. 
A)  Cis-acting factors can bind to specific DNA sequences whereas trans-acting sites are DNA sequences. 
B)  Trans-acting factors can bind to specific DNA sequences whereas cis-acting sites are DNA sequences. 
C)  Trans- and cis-acting factors can both bind to specific DNA sequences. 
D)  Trans- and cis-acting factors can both only bind to DNA elements to which they are physically linked.

Question 14

The phrase trans-acting factors is short for __________ factor protein.

Accepted Answer

A)  translation 
B)  transcription 
C)  transunion 
D)  transform 
A)  translation 
B)  transcription 
C)  transunion 
D)  transform

Question 15

Operons can be defined as units of DNA containing gene(s) under control of __________ promotor(s).

Accepted Answer

A)  one; one 
B)  multiple; one 
C)  multiple; multiple 
D)  one; multiple 
A)  one; one 
B)  multiple; one 
C)  multiple; multiple 
D)  one; multiple

Question 16

Stem cells can undergo two different pathways: cell and cell .

Accepted Answer

A)  proliferation; dedifferentiation 
B)  proliferation; differentiation 
C)  differentiation; dedifferentiation 
D)  differentiation; integration 
A)  proliferation; dedifferentiation 
B)  proliferation; differentiation 
C)  differentiation; dedifferentiation 
D)  differentiation; integration

Question 17

What will happen to the lac operon when there are elevated levels of glucose in a cell?

Accepted Answer

A)  CRP binds strongly. 
B)  Transcription occurs at a very high rate of expression. 
C)  Transcription occurs at a very low rate of expression. 
D)  The lac repressor is bound. 
A)  CRP binds strongly. 
B)  Transcription occurs at a very high rate of expression. 
C)  Transcription occurs at a very low rate of expression. 
D)  The lac repressor is bound.

Question 18

A nucleosome can best be described as a protein core containing molecules each of __________ histones and a segment of DNA.

Accepted Answer

A)  four; four 
B)  two; four 
C)  two; two 
D)  four; two 
A)  four; four 
B)  two; four 
C)  two; two 
D)  four; two

Question 19

Bicoid, Hunchback, Giant, and Kruppel are all transcription factor proteins involved in pattern.

Accepted Answer

A)  even-skipped expression 
B)  odd-skipped expression 
C)  even-skipped transcription 
D)  odd-skipped transcription 
A)  even-skipped expression 
B)  odd-skipped expression 
C)  even-skipped transcription 
D)  odd-skipped transcription

Question 20

List the chain of events that occurs after a pioneer factor binds to a cis-acting sequence.

Accepted Answer

As shown in Figure 23.47 from the text,

Exam 23: Gene Regulation

How is transcription factor recruitment used in eukaryotic gene regulatory circuits?

When IPTG is added to a cell, what is the predicted outcome?

IPTG causes the lac promoter to

When Cro is bound to OR3, the

What are the two main approaches being considered for treatment using iPS cell technology?

When there is no lactose in a cell, production of lac operon

Histone acetylation leads to transcriptional activation by

The best description of how allosteric regulation works is that a ligand the transcriptional regulatory protein, which __________ the protein's affinity for DNA.

Using the figure below, explain how cooperativity works.

The Swi-Snf complex affects chromatin by

The function of RecA* is to

Describe the three biochemical consequences of histone acetylation.

Which of the following best describes how trans- and cis-acting factors operate?

The phrase trans-acting factors is short for __________ factor protein.

Operons can be defined as units of DNA containing gene(s) under control of __________ promotor(s).

Stem cells can undergo two different pathways: cell and cell .

What will happen to the lac operon when there are elevated levels of glucose in a cell?

A nucleosome can best be described as a protein core containing molecules each of __________ histones and a segment of DNA.

Bicoid, Hunchback, Giant, and Kruppel are all transcription factor proteins involved in pattern.

List the chain of events that occurs after a pioneer factor binds to a cis-acting sequence.

Principles of Biochemistry

Physical Biochemistry: Energy Conversion, Water, and Membranes

Nucleic Acid Structure and Function

Protein Structure

Methods in Protein Biochemistry

Protein Function

Enzyme Mechanisms

Cell Signaling Systems

Glycolysis: a Paradigm of Metabolic Regulation

The Citrate Cycle

Oxidative Phosphorylation

Photosynthesis

Carbohydrate Structure and Function

Carbohydrate Metabolism

Lipid Structure and Function

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Metabolic Integration

DNA Replication, Repair, and Recombination

RNA Synthesis, Processing, and Gene Silencing

Protein Synthesis, Posttranslational Modification, and Transport

Filters

When Cro is bound to O_R3, the