Question 1

In eukaryotes,mRNAs are synthesized in the cell ______,whereas most translation occurs in the ______.

Accepted Answer

A) nucleus,nucleosome. 
B) nucleus,cytosol. 
C) cytosol,nucleosome. 
D) cytosol,nucleus. 
E) nucleosome,cytosol. 
A) nucleus,nucleosome. 
B) nucleus,cytosol. 
C) cytosol,nucleosome. 
D) cytosol,nucleus. 
E) nucleosome,cytosol.

Question 2

Matching
Choose the correct answer from the list.Not all the answers will be used.

-Eukaryotic RNA molecules are altered by removal of intervening sequences called ______.

Accepted Answer

A)  Rho (ρ) 
B)  introns 
C)  sigma (σ) 
D)  sense 
E)  operons
F) structural
G) Pribnow
H) promoters
I) antisense
J) exons
K) nucleolus
L) TATA 
M) Sense
N) AAAA (poly 
A) 
O) Ribosome 
A)  Rho (ρ) 
B)  introns 
C)  sigma (σ) 
D)  sense 
E)  operons
F) structural
G) Pribnow
H) promoters
I) antisense
J) exons
K) nucleolus
L) TATA 
M) Sense
N) AAAA (poly 
A) 
O) Ribosome

Question 3

Which of the following is correct regarding splicing?

Accepted Answer

A) CPSFs are products of RNA sequences designed to recognize the 3'OH at the splice site. 
B) PABIII recognizes the A rich sequences at the 5' end of the splice site. 
C) Lariats are the sequence recognized by the cleavage site. 
D) smURFs are used to recognize the poly A tail and initiate slicing 
E) snRNPs are the protein product of U rich RNAs that partially complement the 5' splice junction. 
A) CPSFs are products of RNA sequences designed to recognize the 3'OH at the splice site. 
B) PABIII recognizes the A rich sequences at the 5' end of the splice site. 
C) Lariats are the sequence recognized by the cleavage site. 
D) smURFs are used to recognize the poly A tail and initiate slicing 
E) snRNPs are the protein product of U rich RNAs that partially complement the 5' splice junction.

Question 4

Matching
Choose the correct answer from the list.Not all the answers will be used.

-An AT-rich region found in eukaryotic promoters is called the ______ box.

Accepted Answer

A)  Rho (ρ) 
B)  introns 
C)  sigma (σ) 
D)  sense 
E)  operons
F) structural
G) Pribnow
H) promoters
I) antisense
J) exons
K) nucleolus
L) TATA 
M) Sense
N) AAAA (poly 
A) 
O) Ribosome 
A)  Rho (ρ) 
B)  introns 
C)  sigma (σ) 
D)  sense 
E)  operons
F) structural
G) Pribnow
H) promoters
I) antisense
J) exons
K) nucleolus
L) TATA 
M) Sense
N) AAAA (poly 
A) 
O) Ribosome

Question 5

The precursors of most eukaryotic rRNAs are synthesized by
(I)RNA polymerase I.
(II)RNA polymerase II.
(III)RNA polymerase III.

Accepted Answer

A) I or III 
B) I or II 
C) I only 
D) II only 
E) III only 
A) I or III 
B) I or II 
C) I only 
D) II only 
E) III only

Question 6

In eukaryotes,which of the following would decrease the rate of transcription?

Accepted Answer

A) mutation in the nucleotide sequences at −10 and −35 
B) mutation in the nucleotide sequences at −10 and +5 
C) mutation in the nucleotide sequences at −70 and −90 
D) mutation at the  $\sigma$ binding site 
E) all of the above 
A) mutation in the nucleotide sequences at −10 and −35 
B) mutation in the nucleotide sequences at −10 and +5 
C) mutation in the nucleotide sequences at −70 and −90 
D) mutation at the  $\sigma$ binding site 
E) all of the above

Question 7

The precursors of eukaryotic mRNAs are synthesized by ______.
(I)RNA polymerase I.
(II)RNA polymerase II.
(III)RNA polymerase III.

Accepted Answer

A) I or III 
B) I or II 
C) I only 
D) II only 
E) III only 
A) I or III 
B) I or II 
C) I only 
D) II only 
E) III only

Question 8

How does the preinitiation complex begin to form at a TATA box-containing promoter?

Accepted Answer

A) RNAP binds to the sigma factor. 
B) RNAP binds to the initiator (Inr)element. 
C) TATA-binding protein binds to the TATA box. 
D) RNAP binds to the Rho factor. 
E) none of the above. 
A) RNAP binds to the sigma factor. 
B) RNAP binds to the initiator (Inr)element. 
C) TATA-binding protein binds to the TATA box. 
D) RNAP binds to the Rho factor. 
E) none of the above.

Question 9

Matching
Choose the correct answer from the list.Not all the answers will be used.

-Protein-coding genes are also known as ______ genes.

Accepted Answer

A)  Rho (ρ) 
B)  introns 
C)  sigma (σ) 
D)  sense 
E)  operons
F) structural
G) Pribnow
H) promoters
I) antisense
J) exons
K) nucleolus
L) TATA 
M) Sense
N) AAAA (poly 
A) 
O) Ribosome 
A)  Rho (ρ) 
B)  introns 
C)  sigma (σ) 
D)  sense 
E)  operons
F) structural
G) Pribnow
H) promoters
I) antisense
J) exons
K) nucleolus
L) TATA 
M) Sense
N) AAAA (poly 
A) 
O) Ribosome

Question 10

The Pribnow box is a _____ sequence that is _____ and is located at approximately ____ position of the promoter.

Accepted Answer

A) conserved;AT rich;-10 
B) dispersive;AT rich;-10 
C) conserved;GC rich;-35 
D) dispersive;GC rich;-35 
E) conserved;variable in sequence;0 
A) conserved;AT rich;-10 
B) dispersive;AT rich;-10 
C) conserved;GC rich;-35 
D) dispersive;GC rich;-35 
E) conserved;variable in sequence;0

Question 11

The term rRNA refers to ______ RNA.

Accepted Answer

A) ribosomal 
B) retroviral 
C) recombinant 
D) rho factor 
A) ribosomal 
B) retroviral 
C) recombinant 
D) rho factor

Question 12

Most ______ have well defined 3' ends terminating in poly(A)tails of ~ 250 nt.

Accepted Answer

A) prokaryotic mRNAs 
B) eukaryotic mRNAs 
C) prokaryotic rRNAs 
D) eukaryotic rRNAs 
E) all of the above 
A) prokaryotic mRNAs 
B) eukaryotic mRNAs 
C) prokaryotic rRNAs 
D) eukaryotic rRNAs 
E) all of the above

Question 13

Which of the following is FALSE regarding prokaryotic transcription termination?

Accepted Answer

A) It can occur in a factor dependent fashion. 
B) It can occur in a factor independent fashion. 
C) It may involve the Rho protein. 
D) all of the above 
E) none of the above 
A) It can occur in a factor dependent fashion. 
B) It can occur in a factor independent fashion. 
C) It may involve the Rho protein. 
D) all of the above 
E) none of the above

Question 14

In which of the following types of eukaryotics RNAs are introns completely removed most of the time,although exceptions can occur?
(I)mRNA
(II)rRNA
(III)tRNA

Accepted Answer

A) I,II 
B) I,III 
C) II,III 
D) I,II,III 
E) none of the above 
A) I,II 
B) I,III 
C) II,III 
D) I,II,III 
E) none of the above

Question 15

Matching
Choose the correct answer from the list.Not all the answers will be used.

-Prokaryotic contain nucleotide sequences know as _____ which contain related genes with related functions.

Accepted Answer

A)  Rho (ρ) 
B)  introns 
C)  sigma (σ) 
D)  sense 
E)  operons
F) structural
G) Pribnow
H) promoters
I) antisense
J) exons
K) nucleolus
L) TATA 
M) Sense
N) AAAA (poly 
A) 
O) Ribosome 
A)  Rho (ρ) 
B)  introns 
C)  sigma (σ) 
D)  sense 
E)  operons
F) structural
G) Pribnow
H) promoters
I) antisense
J) exons
K) nucleolus
L) TATA 
M) Sense
N) AAAA (poly 
A) 
O) Ribosome

Question 16

Which of the following types of splicing RNAs uses a free nucleotide to perform a nucleophilic attack?
(I)group I introns
(II)group II introns
(III)self splicing introns

Accepted Answer

A) I,II,III 
B) I,II 
C) I,III 
D) II,III 
E) none of the above 
A) I,II,III 
B) I,II 
C) I,III 
D) II,III 
E) none of the above

Question 17

Splicing
(I)occurs in the cytosol.
(II)results from 2' -OH on the exon attack on the 5' splice site.
(III)is completed when the 3' -OH on the newly cut exon attacks the 5'-terminal phosphate on the next exon.

Accepted Answer

A) I,II 
B) I,III 
C) I only 
D) II only 
E) III only 
A) I,II 
B) I,III 
C) I only 
D) II only 
E) III only

Question 18

The precursors of 5S rRNA,the tRNAs,and a variety of other small nuclear and cytosolic RNAs are synthesized by ______.
(I)RNA polymerase I.
(II)RNA polymerase II.
(III)RNA polymerase III.

Accepted Answer

A) I or III 
B) I or II 
C) I only 
D) II only 
E) III only 
A) I or III 
B) I or II 
C) I only 
D) II only 
E) III only

Question 19

The fact that some eukaryotic rRNAs are self-splicing indicates that

Accepted Answer

A) RNA structures are highly variable. 
B) RNAs can contain modified bases. 
C) eukaryotes have multiple RNA polymerases. 
D) RNA can act as an enzyme or catalyst. 
E) None of the above is correct. 
A) RNA structures are highly variable. 
B) RNAs can contain modified bases. 
C) eukaryotes have multiple RNA polymerases. 
D) RNA can act as an enzyme or catalyst. 
E) None of the above is correct.

Question 20

Which of the following types of RNA undergo posttranscriptional modifications?

Accepted Answer

A) mRNA 
B) rRNA 
C) tRNA 
D) all of the above 
E) none of the above 
A) mRNA 
B) rRNA 
C) tRNA 
D) all of the above 
E) none of the above

Exam 26: Transcription and Rna Processing

In eukaryotes,mRNAs are synthesized in the cell ______,whereas most translation occurs in the ______.

Matching Choose the correct answer from the list.Not all the answers will be used. -Eukaryotic RNA molecules are altered by removal of intervening sequences called ______.

Which of the following is correct regarding splicing?

Matching Choose the correct answer from the list.Not all the answers will be used. -An AT-rich region found in eukaryotic promoters is called the ______ box.

The precursors of most eukaryotic rRNAs are synthesized by (I)RNA polymerase I. (II)RNA polymerase II. (III)RNA polymerase III.

In eukaryotes,which of the following would decrease the rate of transcription?

The precursors of eukaryotic mRNAs are synthesized by ______. (I)RNA polymerase I. (II)RNA polymerase II. (III)RNA polymerase III.

How does the preinitiation complex begin to form at a TATA box-containing promoter?

Matching Choose the correct answer from the list.Not all the answers will be used. -Protein-coding genes are also known as ______ genes.

The Pribnow box is a _____ sequence that is _____ and is located at approximately ____ position of the promoter.

The term rRNA refers to ______ RNA.

Most ______ have well defined 3' ends terminating in poly(A)tails of ~ 250 nt.

Which of the following is FALSE regarding prokaryotic transcription termination?

In which of the following types of eukaryotics RNAs are introns completely removed most of the time,although exceptions can occur? (I)mRNA (II)rRNA (III)tRNA

Matching Choose the correct answer from the list.Not all the answers will be used. -Prokaryotic contain nucleotide sequences know as _____ which contain related genes with related functions.

Which of the following types of splicing RNAs uses a free nucleotide to perform a nucleophilic attack? (I)group I introns (II)group II introns (III)self splicing introns

Splicing (I)occurs in the cytosol. (II)results from 2' -OH on the exon attack on the 5' splice site. (III)is completed when the 3' -OH on the newly cut exon attacks the 5'-terminal phosphate on the next exon.

The precursors of 5S rRNA,the tRNAs,and a variety of other small nuclear and cytosolic RNAs are synthesized by ______. (I)RNA polymerase I. (II)RNA polymerase II. (III)RNA polymerase III.

The fact that some eukaryotic rRNAs are self-splicing indicates that

Which of the following types of RNA undergo posttranscriptional modifications?

Introduction to the Chemistry of Life

Water

Nucleotides, nucleic Acids, and Genetic Information

Amino Acids

Proteins: Primary Structure

Proteins: Three-Dimensional Structure

Protein Function Part I: Myoglobin and Hemoglobin

Carbohydrates

Lipids and Biological Membranes

Membrane Transport

Enzyme Catalysis

Enzyme Kinetics, inhibition and Control

Biochemical Signaling

Introduction to Metabolism

Glucose Catabolism

Glycogen Metabolism and Gluconeogenesis

Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Mammalian Fuel Metabolism: Integration and Regulation

Nucleotide Metabolism

Nucleic Acid Structure

DNA Replication, repair and Recombination

Protein Synthesis

Regulation of Gene Expression

Filters

In eukaryotes,mRNAs are synthesized in the cell ,whereas most translation occurs in the .

The Pribnow box is a ___ sequence that is _ and is located at approximately __ position of the promoter.