Exam 3: Nucleotides, nucleic Acids, and Genetic Information

DNA sequencing by the chain-termination method utilizes DNA polymerase I to make a complementary copy of the target or template DNA molecule.A reaction with a 20 bp template and dideoxyadenosine nucleotides as terminators results in the production of a 5 bp fragment.Based on this result,we can conclude that the template contains

In living organisms,genetic information is most often stored in the form of

Matching -After hybridization,the fragment of interest can be detected by ______.

DNA sequencing using the Sanger method requires

This is the structure of adenine. a.Is adenine a purine or a pyrimidine? b.Which base does adenine base-pair (H-bond)with in DNA? c.What is the name of the molecule that is composed of adenine linked to the C1' of ribose? d.Indicate on the drawing through which atom adenine is connected to ribose or deoxyribose. e.Indicate on the drawing which groups on adenine are involved in base-pairing or H-bonding with its complementary base.

The molecule shown in the diagram is a

In Sanger dideoxy DNA sequencing,DNA polymerase I is used to add nucleotides to the ___ end of the growing polynucleotide chain.

Which of the following DNA sequences is considered palindromic?

Nucleotides in a DNA molecule are linked together through

Which of the restriction enzymes listed in the table below produces blunt-end fragments?

Small circular DNA molecules used to carry foreign DNA fragments are called ________.

Matching -The method that Sanger developed for DNA sequencing using dideoxy nucleotides is called the ______ method.

Matching -Organisms composed of cells with two sets of chromosomes are called ______ organisms.

What is the name of the molecule shown in the diagram?

Which of the following molecules does not contain an energy rich phosphoanhydride bond?

In 1944 Avery,MacLeod,and McCarty did experiments with Diplococcus pneumoniae that proved that

Which of the enzymes listed in the table below produce identical sticky ends?

Matching -A small fragment of labeled DNA or RNA used in a hybridization experiment is called a ______.

In order to perform PCR,which of the following describes the reagents that must be included in the reaction mixture?

DNA polymerase from Thermus aquaticus is used in PCR because