Exam 12: Enzyme Kinetics, inhibition and Control

A lab recently developed a new drug which is hypothesized to inhibit the enzyme cyclooxygenase-2 (COX-2)and reduce inflammation.In their first test they monitored the reaction of substrate as it is converted to product in the presence of the new drug (data shown below).If the hypothesis is correct the observed initial rate will be at least 2 times slower than the normal reaction without the drug.If the normal initial rate is 30 mM/s,does the data below indicate that the team has designed a successful inhibitor?

The following data were collected under conditions indicated in the graph below during the time period of 0-5 seconds.Upon plotting the Lineweaver-Burk plot,the information given in the table below was determined.Based on this available information which of the following is FALSE? X-intercept - 0.002 (Units on the x-axis are 1/M) Y-intercept 0.005 (Units on the y-axis are 1/s) Slope 2.50

Allosteric activators

K_M

Reaction that is first order with respect to A and B

The catalytic efficiency of an enzyme can never exceed

An extremely efficient enzyme called "efficase" catalyzes the conversion of "A" to "B." A researcher decides to mutate the enzyme in order to try to improve its performance.Following active site mutations,a significant reduction in the value of K_M and V_max was observed.Which of the following may have occurred?

Parallel lines on a Lineweaver-Burk plot indicate (I)an increase in K_M. (II)decrease in K_M. (III)decrease in V_max. (IV)uncompetitive inhibition.

Matching -The E+S $\to$ E+P reaction is ______.

Matching -Assume a first order reaction,the rate of the reaction 2A $\to$ B is dependent on ______.

Matching -A lead compound for a new drug should bind to its target protein with a very ______.

Find the initial velocity for an enzymatic reaction when V_max = 6.5 × 10^-5 mol•sec^-1,[S] = 3.0 × 10^-3 M,K_M = 4.5 × 10^-3 M and the enzyme concentration at time zero is 1.5 × 10^-2 $\mu$ M.

Following several experiments,the data presented on the graph below was obtained.What can you determine from this graph?

The following questions refer to the diagram (with boxes where it has been left incomplete): -Which of the following is correct in regards to the diagram above?