Question 1

Which of the following best describes placing a sample of body secretions on a special medium to allow bacteria to grow?

Accepted Answer

A) Sensitivity test 
B) Culture 
C) Holding media 
D) Mordant 
A) Sensitivity test 
B) Culture 
C) Holding media 
D) Mordant B

Question 2

Which of the following best describes the use of the colony streaking technique?

Accepted Answer

A) Dilute bacteria 
B) Grow yeast 
C) Plate urine cultures 
D) Grow Shigella 
A) Dilute bacteria 
B) Grow yeast 
C) Plate urine cultures 
D) Grow Shigella C

Question 3

Which of the following is the type of stain that is often referred to as a specific stain and that allows for microscopic examination of acid-fast organisms?

Accepted Answer

A) Gram 
B) Acid-fast 
C) Simple 
D) None of the above 
A) Gram 
B) Acid-fast 
C) Simple 
D) None of the above B

Question 4

Which of the following is the KOH used for testing?

Accepted Answer

A) Gram positive bacteria 
B) Acid-fast bacteria 
C) Fungi and spores 
D) Stool specimens 
A) Gram positive bacteria 
B) Acid-fast bacteria 
C) Fungi and spores 
D) Stool specimens

Question 5

What is the organism that causes strep throat?

Accepted Answer

A) Group A, alpha-hemolytic streptococcus 
B) Group A, beta-hemolytic streptococcus 
C) Group B, alpha-hemolytic streptococcus 
D) Group B, beta-hemolytic streptococcus 
A) Group A, alpha-hemolytic streptococcus 
B) Group A, beta-hemolytic streptococcus 
C) Group B, alpha-hemolytic streptococcus 
D) Group B, beta-hemolytic streptococcus

Question 6

Which of the following bacteria are purple rods when the staining process is complete?

Accepted Answer

A) Gram-negative bacilli 
B) Gram-positive bacilli 
C) Gram-positive cocci 
D) Gram-negative spirilla 
A) Gram-negative bacilli 
B) Gram-positive bacilli 
C) Gram-positive cocci 
D) Gram-negative spirilla

Question 7

If a spill should occur in the lab, which of the the following agents is used for cleanup:

Accepted Answer

A) 2% phenol 
B) 10% bleach 
C) 10% phenol 
D) 3% bleach 
A) 2% phenol 
B) 10% bleach 
C) 10% phenol 
D) 3% bleach

Question 8

A wet mount is used to:

Accepted Answer

A) Stain bacteria 
B) Suspend the organism in a liquid to study 
C) Examine organisms under a microscope 
D) Both b and c 
A) Stain bacteria 
B) Suspend the organism in a liquid to study 
C) Examine organisms under a microscope 
D) Both b and c

Question 9

Which of the following is NOT given out as patient education to relieve discomfort and help patients toward better health?

Accepted Answer

A) Dress comfortably according to the weather. 
B) Avoid tobacco. 
C) Cough or sneeze into a tissue. 
D) Decrease water intake. 
A) Dress comfortably according to the weather. 
B) Avoid tobacco. 
C) Cough or sneeze into a tissue. 
D) Decrease water intake.

Question 10

Which of the following is NOT part of a quality control program in microbiology?

Accepted Answer

A) Monitor all equipment with temperature controls daily. 
B) Do not use media past the shelf life. 
C) Update the laboratory manual daily. 
D) Make the SDS available to reference. 
A) Monitor all equipment with temperature controls daily. 
B) Do not use media past the shelf life. 
C) Update the laboratory manual daily. 
D) Make the SDS available to reference.

Question 11

Mycology is the study of

Accepted Answer

A) Protozoa 
B) Algae 
C) Bacteria 
D) Fungi 
A) Protozoa 
B) Algae 
C) Bacteria 
D) Fungi

Question 12

After the agar plate has been inoculated and properly labeled, what must you do with the plate?

Accepted Answer

A) Nothing, agar plates require nothing special. 
B) Place it back with the other agar plates in the original package. 
C) Leave it right-side-up, and place in the proper environment for growth. 
D) Turn it upside down, and place in the proper environment for growth. 
A) Nothing, agar plates require nothing special. 
B) Place it back with the other agar plates in the original package. 
C) Leave it right-side-up, and place in the proper environment for growth. 
D) Turn it upside down, and place in the proper environment for growth.

Question 13

Which of the following type of specimen is obtained when a lumbar puncture is performed by the physician?

Accepted Answer

A) Sputum 
B) Stool 
C) Mucous 
D) Cerebrospinal fluid 
A) Sputum 
B) Stool 
C) Mucous 
D) Cerebrospinal fluid

Question 14

Which of the following applies to the collection of specimens?

Accepted Answer

A) Lab assistants do not need to wear gloves. 
B) Wear gloves only when needed. 
C) Occasionally follow instructions in the lab manual. 
D) Must strictly adhere to standard precautions. 
A) Lab assistants do not need to wear gloves. 
B) Wear gloves only when needed. 
C) Occasionally follow instructions in the lab manual. 
D) Must strictly adhere to standard precautions.

Question 15

When using KOH on a wet mount, what does the KOH do?

Accepted Answer

A) Stains the organism 
B) Destroys the fungus 
C) Clears debris 
D) Makes objects look brighter 
A) Stains the organism 
B) Destroys the fungus 
C) Clears debris 
D) Makes objects look brighter

Question 16

Which of the following should be used when obtaining a nasopharyngeal collection for a culture?

Accepted Answer

A) Sterile tongue depressor 
B) Sterile applicator stick 
C) Special swab on a thin wire 
D) None of the above 
A) Sterile tongue depressor 
B) Sterile applicator stick 
C) Special swab on a thin wire 
D) None of the above

Question 17

Media that that contains substances that alter the appearance of some organisms and not others is called:

Accepted Answer

A) Differential media 
B) Basic media 
C) Selective media 
D) Enriched media 
A) Differential media 
B) Basic media 
C) Selective media 
D) Enriched media

Question 18

Giardiasis Lamblia is often contracted by which of the following?

Accepted Answer

A) Mountain hikers 
B) Children 
C) Sanitation workers 
D) Athletes 
A) Mountain hikers 
B) Children 
C) Sanitation workers 
D) Athletes

Question 19

Which of the following is the next step when there is more than one pathogen growing in a bacterial culture?

Accepted Answer

A) Use a dissecting microscope. 
B) Wait an additional 36 hours to identify the predominant organism. 
C) Obtain another specimen. 
D) Set up a subculture and separate bacteria to another media. 
A) Use a dissecting microscope. 
B) Wait an additional 36 hours to identify the predominant organism. 
C) Obtain another specimen. 
D) Set up a subculture and separate bacteria to another media.

Question 20

Which of the following methods is used for maintaining an anaerobic condition?

Accepted Answer

A) Incubator 
B) Gas pack jar 
C) Petri dish 
D) Safety hood 
A) Incubator 
B) Gas pack jar 
C) Petri dish 
D) Safety hood

Exam 42: Basic Microbiology

Which of the following best describes placing a sample of body secretions on a special medium to allow bacteria to grow?

Which of the following best describes the use of the colony streaking technique?

Which of the following is the type of stain that is often referred to as a specific stain and that allows for microscopic examination of acid-fast organisms?

Which of the following is the KOH used for testing?

What is the organism that causes strep throat?

Which of the following bacteria are purple rods when the staining process is complete?

If a spill should occur in the lab, which of the the following agents is used for cleanup:

A wet mount is used to:

Which of the following is NOT given out as patient education to relieve discomfort and help patients toward better health?

Which of the following is NOT part of a quality control program in microbiology?

Mycology is the study of

After the agar plate has been inoculated and properly labeled, what must you do with the plate?

Which of the following type of specimen is obtained when a lumbar puncture is performed by the physician?

Which of the following applies to the collection of specimens?

When using KOH on a wet mount, what does the KOH do?

Which of the following should be used when obtaining a nasopharyngeal collection for a culture?

Media that that contains substances that alter the appearance of some organisms and not others is called:

Giardiasis Lamblia is often contracted by which of the following?

Which of the following is the next step when there is more than one pathogen growing in a bacterial culture?

Which of the following methods is used for maintaining an anaerobic condition?

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