Question 1

Which category of media can be described as containing one or more agents that are inhibitory to all organisms except those being sought?

Accepted Answer

A)  Enrichment 
B)  Supportive 
C)  Selective 
D)  Differential 
A)  Enrichment 
B)  Supportive 
C)  Selective 
D)  Differential

Question 2

Which group of bacteria uses oxygen as a terminal electron acceptor and grows well in ambient air?

Accepted Answer

A)  Aerobes 
B)  Anaerobes 
C)  Capnophiles 
D)  Microaerophiles 
A)  Aerobes 
B)  Anaerobes 
C)  Capnophiles 
D)  Microaerophiles

Question 3

The role of the agar ingredient in culture medium is to:

Accepted Answer

A)  provide a nutritional base for bacterial metabolism. 
B)  solidify the medium and provide a stable culture surface. 
C)  sterilize the medium and prevent contamination. 
D)  support the growth of only pathogenic organisms. 
A)  provide a nutritional base for bacterial metabolism. 
B)  solidify the medium and provide a stable culture surface. 
C)  sterilize the medium and prevent contamination. 
D)  support the growth of only pathogenic organisms.

Question 4

A positive pyrrolidonyl arylamidase (PYR)test was observed on a species of Enterococcus.What color should be observed for this reaction?

Accepted Answer

A)  Purple 
B)  Red 
C)  Green 
D)  Pink 
A)  Purple 
B)  Red 
C)  Green 
D)  Pink

Question 5

Which medium can be described as suppressing the growth of most gram-negative organisms while allowing gram-positive bacteria to grow?

Accepted Answer

A)  Chocolate 
B)  CNA blood 
C)  BHI 
D)  Thioglycollate 
A)  Chocolate 
B)  CNA blood 
C)  BHI 
D)  Thioglycollate

Question 6

To read the hemolytic reaction accurately on a blood agar plate, the technologist must:

Accepted Answer

A)  hold the plate up to the light, and observe the plate with the light coming from behind. 
B)  hold the plate under the light, and observe the plate with the light coming from the top. 
C)  hold the plate over a white background. 
D)  examine the plate using reflected and transmitted light. 
A)  hold the plate up to the light, and observe the plate with the light coming from behind. 
B)  hold the plate under the light, and observe the plate with the light coming from the top. 
C)  hold the plate over a white background. 
D)  examine the plate using reflected and transmitted light.

Question 7

Organisms that have nutritional needs that are relatively complex and require extra media components to be used for growth are called:

Accepted Answer

A)  fastidious. 
B)  capnophiles. 
C)  neutrophils. 
D)  halophiles. 
A)  fastidious. 
B)  capnophiles. 
C)  neutrophils. 
D)  halophiles.

Question 8

Generation time can be defined as the:

Accepted Answer

A)  time required for a bacterial population to double. 
B)  time required for a bacterial population to triple. 
C)  time required for a bacterial population to quadruple. 
D)  half-life of bacteria. 
A)  time required for a bacterial population to double. 
B)  time required for a bacterial population to triple. 
C)  time required for a bacterial population to quadruple. 
D)  half-life of bacteria.

Question 9

A candle jar is used to cultivate which type of bacteria?

Accepted Answer

A)  Aerobes 
B)  Anaerobes 
C)  Capnophiles 
D)  Microaerophiles 
A)  Aerobes 
B)  Anaerobes 
C)  Capnophiles 
D)  Microaerophiles

Question 10

Oxidative or fermentative media were set up on an organism suspected of being Pseudomonas aeruginosa.What results would be expected?

Accepted Answer

A)  Yellow in both tubes 
B)  Green in both tubes 
C)  Yellow in the open tube and green in the closed tube 
D)  Green in the open tube and yellow in the closed tube 
A)  Yellow in both tubes 
B)  Green in both tubes 
C)  Yellow in the open tube and green in the closed tube 
D)  Green in the open tube and yellow in the closed tube

Exam 7: Traditional Cultivation and Identification

Which category of media can be described as containing one or more agents that are inhibitory to all organisms except those being sought?

Which group of bacteria uses oxygen as a terminal electron acceptor and grows well in ambient air?

The role of the agar ingredient in culture medium is to:

A positive pyrrolidonyl arylamidase (PYR)test was observed on a species of Enterococcus.What color should be observed for this reaction?

Which medium can be described as suppressing the growth of most gram-negative organisms while allowing gram-positive bacteria to grow?

To read the hemolytic reaction accurately on a blood agar plate, the technologist must:

Organisms that have nutritional needs that are relatively complex and require extra media components to be used for growth are called:

Generation time can be defined as the:

A candle jar is used to cultivate which type of bacteria?

Oxidative or fermentative media were set up on an organism suspected of being Pseudomonas aeruginosa.What results would be expected?

Microbial Taxonomy

Bacterial Genetics, Metabolism, and Structure

Host-Microorganism Interactions

Laboratory Safety

Specimen Management

Role of Microscopy

Nucleic Acid-Based Analytic Methods for Microbial Identification and Characterization

Immunochemical Methods Used for Organism Detection

Serologic Diagnosis of Infectious Diseases

Principles of Antimicrobial Action and Resistance

Laboratory Methods and Strategies for Antimicrobial Susceptibility Testing

Staphylococcus Micrococcus and Similar Organisms

Streptococcus Enterococcus and Similar Organisms

Bacillus and Similar Organisms

Listeria Corynebacterium and Similar Organisms

Erysipelothrix Lactobacillus and Similar Organisms

Nocardia Streptomyces Rhodococcus and Similar Organisms

Enterobacteriaceae

Acinetobacter, Stenotrophomonas, and Other Organisms

Pseudomonas, Burkholderia, and Similar Organisms

Rhizobium, Ochrobactrum, and Similar Organisms

Chryseobacterium, Sphingobacterium, and Similar Organisms

Alcaligenes, Bordetella Nonpertussis, Comamonas, and Similar Organisms

Vibrio, Aeromonas, Chromobacterium, and Related Organisms

Sphingomonas Paucimobilis and Similar Organisms

Moraxella and Related Organisms

Eikenella and Similar Organisms

Pasteurella and Similar Organisms

Actinobacillus, Aggregatibacter, Kingella, Cardiobacterium, Capnocytophaga, and Similar Organisms

Haemophilus

Bartonella and Afipia

Campylobacter, Arcobacter, and Helicobacter

Legionella

Brucella

Bordetella Pertussis, Bordetella Parapertussis, and Related Species

Francisella

Streptobacillus Moniliformis and Spirillum Minus

Neisseria and Moraxella Catarrhalis

Overview and General Considerations

Overview of Anaerobic Organisms

Mycobacteria

Obligate Intracellular and Non-culturable Bacterial Agents

Cell Walldeficient Bacteria: Mycoplasma and Ureaplasma

The Spirochetes

Laboratory Methods for Diagnosis of Parasitic Infections: Overview

Intestinal Protozoa

Blood and Tissue Protozoa

Other Protozoa

Intestinal Nematodes Roundworms

Tissue Nematodes Roundworms

Blood and Tissue Filarialnematodes

Intestinal Cestodes

Tissue Cestodes

Intestinal Trematodes

Liver and Lung Trematodes

Blood Trematodes

Overview of Fungal Identification Methods and Strategies

Hyaline Molds, Mucorales Zygomycetes, Dermatophytes, and Opportunitistic and Systemic Mycoses

Dematiaceous Melanizedmolds

Opportunistic Atypical Fungus: Pneumocystis Jiroveci

The Yeasts

Anti-fungal Susceptibility Testing, Therapy, and Prevention

Overview of the Methods and Strategies in Virology

Viruses in Human Disease

Antiviral Therapy, Susceptibility Testing, and Prevention

Bloodstream Infections

Infections of the Lower Respiratory Tract

Upper Respiratory Tract Infections and Other Infections of the Oral Cavity and Neck

Meningitis, Encephalitis, and Other Infections of the Central Nervous System