Question 1

An automated system uses bead-capture technology in the detection of which of the following spirochetes?

Accepted Answer

A)  Treponema 
B)  Borrelia 
C)  Leptospira 
D)  Brachyspira 
A)  Treponema 
B)  Borrelia 
C)  Leptospira 
D)  Brachyspira A

Question 2

The most severe illness associated with leptospirae, with symptoms of liver, kidney, and/or vascular dysfunction with lethal pulmonary hemorrhage and sometimes death, is called:

Accepted Answer

A)  icteric leptospirosis. 
B)  anicteric leptospirosis. 
C)  Vincent's disease. 
D)  syphilis. 
A)  icteric leptospirosis. 
B)  anicteric leptospirosis. 
C)  Vincent's disease. 
D)  syphilis. A

Question 3

MATCHING
Match the component with the assay with which it is associated.
-Gelatin particles

Accepted Answer

A) TP-PA 
B) MHA-TP 
C) PaGIA 
D) Enzyme immunoassay (EI
A)  
E) Magnetic beads 
A) TP-PA 
B) MHA-TP 
C) PaGIA 
D) Enzyme immunoassay (EI
A)  
E) Magnetic beads A

Question 4

Treponemal antibodies are those that are:

Accepted Answer

A)  produced against antigens of the organisms themselves. 
B)  produced by infected patients against components of mammalian cells. 
C)  produced by the organism that causes the systemic destruction of the disease. 
D)  also called reaginic antibodies. 
A)  produced against antigens of the organisms themselves. 
B)  produced by infected patients against components of mammalian cells. 
C)  produced by the organism that causes the systemic destruction of the disease. 
D)  also called reaginic antibodies.

Question 5

A patient has a reactive RPR result and a positive FTA-ABS test.The drug of choice to treat this infection is:

Accepted Answer

A)  penicillin G. 
B)  tetracycline. 
C)  clindamycin. 
D)  erythromycin. 
A)  penicillin G. 
B)  tetracycline. 
C)  clindamycin. 
D)  erythromycin.

Question 6

MATCHING
Match the component with the assay with which it is associated.
-Recombinant antigens

Accepted Answer

A) TP-PA 
B) MHA-TP 
C) PaGIA 
D) Enzyme immunoassay (EI
A)  
E) Magnetic beads 
A) TP-PA 
B) MHA-TP 
C) PaGIA 
D) Enzyme immunoassay (EI
A)  
E) Magnetic beads

Question 7

Nontreponemal antibodies are those that are:

Accepted Answer

A)  produced against antigens of the organisms themselves. 
B)  produced by infected patients against components of mammalian cells. 
C)  produced by the organism that causes the systemic destruction of the disease. 
D)  also called heterophile antibodies. 
A)  produced against antigens of the organisms themselves. 
B)  produced by infected patients against components of mammalian cells. 
C)  produced by the organism that causes the systemic destruction of the disease. 
D)  also called heterophile antibodies.

Question 8

MATCHING
Match the component with the assay with which it is associated.
-Gel immunoassay

Accepted Answer

A) TP-PA 
B) MHA-TP 
C) PaGIA 
D) Enzyme immunoassay (EI
A)  
E) Magnetic beads 
A) TP-PA 
B) MHA-TP 
C) PaGIA 
D) Enzyme immunoassay (EI
A)  
E) Magnetic beads

Question 9

The most common vector-borne disease in North America and Europe transmitted by the bite of Ixodes ticks is caused by:

Accepted Answer

A)  Leptospira interrogans. 
B)  Borrelia burgdorferi. 
C)  Treponema carateum. 
D)  Rickettsia rickettsii. 
A)  Leptospira interrogans. 
B)  Borrelia burgdorferi. 
C)  Treponema carateum. 
D)  Rickettsia rickettsii.

Question 10

Long, slender, helically curved, gram-negative bacilli with tight coils are identified as belonging to which species?

Accepted Answer

A)  Treponema 
B)  Leptospira 
C)  Borrelia 
D)  Coxiella 
A)  Treponema 
B)  Leptospira 
C)  Borrelia 
D)  Coxiella

Question 11

Long, slender, helically curved, gram-negative bacilli with hooked ends are identified as belonging to which species?

Accepted Answer

A)  Treponema 
B)  Leptospira 
C)  Borrelia 
D)  Coxiella 
A)  Treponema 
B)  Leptospira 
C)  Borrelia 
D)  Coxiella

Question 12

A test that uses gelatin particles sensitized with T.pallidum subsp.pallidum antigens to detect specific antitreponemal antibodies in patient serum is called the __________ test.

Accepted Answer

A)  RPR 
B)  FTA-ABS 
C)  VDRL 
D)  TP-PA 
A)  RPR 
B)  FTA-ABS 
C)  VDRL 
D)  TP-PA

Question 13

A destructive lesion of the gums, also known as acute necrotizing ulcerative gingivitis, that is caused by oral spirochetes and other bacteria is known as:

Accepted Answer

A)  Vincent's disease. 
B)  primary syphilis. 
C)  chancres. 
D)  Weil syndrome. 
A)  Vincent's disease. 
B)  primary syphilis. 
C)  chancres. 
D)  Weil syndrome.

Question 14

A widely used nontreponemal serologic assay is the ________ test.

Accepted Answer

A)  rapid plasma reagin (RPR) 
B)  Treponema pallidum passive particle agglutination (TP-P
A)  
C)  fluorescent treponemal antibody absorption test (FTA-ABS) 
D)  microhemagglutination test for antibody to Treponema pallidum (MHA-TP) 
A)  rapid plasma reagin (RPR) 
B)  Treponema pallidum passive particle agglutination (TP-P
A)  
C)  fluorescent treponemal antibody absorption test (FTA-ABS) 
D)  microhemagglutination test for antibody to Treponema pallidum (MHA-TP)

Question 15

Systemic symptoms, such as fever, weight loss, malaise, and a loss of appetite, along with a widespread rash, can be observed in patients suffering from _________ syphilis.

Accepted Answer

A)  primary 
B)  secondary 
C)  tertiary 
D)  latent 
A)  primary 
B)  secondary 
C)  tertiary 
D)  latent

Question 16

MATCHING
Match the component with the assay with which it is associated.
-Erythrocytes

Accepted Answer

A) TP-PA 
B) MHA-TP 
C) PaGIA 
D) Enzyme immunoassay (EI
A)  
E) Magnetic beads 
A) TP-PA 
B) MHA-TP 
C) PaGIA 
D) Enzyme immunoassay (EI
A)  
E) Magnetic beads

Question 17

A specific treponemal serologic test performed by overlaying whole treponemes that are fixed to a slide with serum from patients suspected of having syphilis and using fluorescein-conjugated antihuman antibody reagent to detect specific antitreponemal antibodies is called the _________ test.

Accepted Answer

A)  RPR 
B)  FTA-ABS 
C)  VDRL 
D)  TP-PA 
A)  RPR 
B)  FTA-ABS 
C)  VDRL 
D)  TP-PA

Question 18

A stage of venereal syphilis characterized by the appearance of a chancre is known as __________ syphilis.

Accepted Answer

A)  primary 
B)  secondary 
C)  late 
D)  tertiary 
A)  primary 
B)  secondary 
C)  late 
D)  tertiary

Question 19

MATCHING
Match the component with the assay with which it is associated.
-Isoluminol-antigen conjugate

Accepted Answer

A) TP-PA 
B) MHA-TP 
C) PaGIA 
D) Enzyme immunoassay (EI
A)  
E) Magnetic beads 
A) TP-PA 
B) MHA-TP 
C) PaGIA 
D) Enzyme immunoassay (EI
A)  
E) Magnetic beads

Question 20

The Centers for Disease Control and Prevention (CDC)recommendations for testing in suspected cases of Lyme disease are to screen with ______ and confirm with ________.

Accepted Answer

A)  RPR; FTA-ABS 
B)  enzyme-linked immunosorbent assay (ELIS
A) ; immunofluorescent antibody (IF
A)  
C)  IFA; immunoblotting 
D)  ELISA; TP-PA 
A)  RPR; FTA-ABS 
B)  enzyme-linked immunosorbent assay (ELIS
A) ; immunofluorescent antibody (IF
A)  
C)  IFA; immunoblotting 
D)  ELISA; TP-PA

Exam 45: The Spirochetes

An automated system uses bead-capture technology in the detection of which of the following spirochetes?

The most severe illness associated with leptospirae, with symptoms of liver, kidney, and/or vascular dysfunction with lethal pulmonary hemorrhage and sometimes death, is called:

MATCHING Match the component with the assay with which it is associated. -Gelatin particles

Treponemal antibodies are those that are:

A patient has a reactive RPR result and a positive FTA-ABS test.The drug of choice to treat this infection is:

MATCHING Match the component with the assay with which it is associated. -Recombinant antigens

Nontreponemal antibodies are those that are:

MATCHING Match the component with the assay with which it is associated. -Gel immunoassay

The most common vector-borne disease in North America and Europe transmitted by the bite of Ixodes ticks is caused by:

Long, slender, helically curved, gram-negative bacilli with tight coils are identified as belonging to which species?

Long, slender, helically curved, gram-negative bacilli with hooked ends are identified as belonging to which species?

A test that uses gelatin particles sensitized with T.pallidum subsp.pallidum antigens to detect specific antitreponemal antibodies in patient serum is called the __________ test.

A destructive lesion of the gums, also known as acute necrotizing ulcerative gingivitis, that is caused by oral spirochetes and other bacteria is known as:

A widely used nontreponemal serologic assay is the ________ test.

Systemic symptoms, such as fever, weight loss, malaise, and a loss of appetite, along with a widespread rash, can be observed in patients suffering from _________ syphilis.

MATCHING Match the component with the assay with which it is associated. -Erythrocytes

A specific treponemal serologic test performed by overlaying whole treponemes that are fixed to a slide with serum from patients suspected of having syphilis and using fluorescein-conjugated antihuman antibody reagent to detect specific antitreponemal antibodies is called the _________ test.

A stage of venereal syphilis characterized by the appearance of a chancre is known as __________ syphilis.

MATCHING Match the component with the assay with which it is associated. -Isoluminol-antigen conjugate

The Centers for Disease Control and Prevention (CDC)recommendations for testing in suspected cases of Lyme disease are to screen with ______ and confirm with ________.

Microbial Taxonomy

Bacterial Genetics, Metabolism, and Structure

Host-Microorganism Interactions

Laboratory Safety

Specimen Management

Role of Microscopy

Traditional Cultivation and Identification

Nucleic Acid-Based Analytic Methods for Microbial Identification and Characterization

Immunochemical Methods Used for Organism Detection

Serologic Diagnosis of Infectious Diseases

Principles of Antimicrobial Action and Resistance

Laboratory Methods and Strategies for Antimicrobial Susceptibility Testing

Staphylococcus Micrococcus and Similar Organisms

Streptococcus Enterococcus and Similar Organisms

Bacillus and Similar Organisms

Listeria Corynebacterium and Similar Organisms

Erysipelothrix Lactobacillus and Similar Organisms

Nocardia Streptomyces Rhodococcus and Similar Organisms

Enterobacteriaceae

Acinetobacter, Stenotrophomonas, and Other Organisms

Pseudomonas, Burkholderia, and Similar Organisms

Rhizobium, Ochrobactrum, and Similar Organisms

Chryseobacterium, Sphingobacterium, and Similar Organisms

Alcaligenes, Bordetella Nonpertussis, Comamonas, and Similar Organisms

Vibrio, Aeromonas, Chromobacterium, and Related Organisms

Sphingomonas Paucimobilis and Similar Organisms

Moraxella and Related Organisms

Eikenella and Similar Organisms

Pasteurella and Similar Organisms

Actinobacillus, Aggregatibacter, Kingella, Cardiobacterium, Capnocytophaga, and Similar Organisms

Haemophilus

Bartonella and Afipia

Campylobacter, Arcobacter, and Helicobacter

Legionella

Brucella

Bordetella Pertussis, Bordetella Parapertussis, and Related Species

Francisella

Streptobacillus Moniliformis and Spirillum Minus

Neisseria and Moraxella Catarrhalis

Overview and General Considerations

Overview of Anaerobic Organisms

Mycobacteria

Obligate Intracellular and Non-culturable Bacterial Agents

Cell Walldeficient Bacteria: Mycoplasma and Ureaplasma

Laboratory Methods for Diagnosis of Parasitic Infections: Overview

Intestinal Protozoa

Blood and Tissue Protozoa

Other Protozoa

Intestinal Nematodes Roundworms

Tissue Nematodes Roundworms

Blood and Tissue Filarialnematodes

Intestinal Cestodes

Tissue Cestodes

Intestinal Trematodes

Liver and Lung Trematodes

Blood Trematodes

Overview of Fungal Identification Methods and Strategies

Hyaline Molds, Mucorales Zygomycetes, Dermatophytes, and Opportunitistic and Systemic Mycoses

Dematiaceous Melanizedmolds

The Centers for Disease Control and Prevention (CDC)recommendations for testing in suspected cases of Lyme disease are to screen with and confirm with __.