Exam 7: Traditional Cultivation and Identification

The most medically important bacteria sought in the medical laboratory are cultivated using incubators with temperatures maintained in what range?

Which medium can be described as a nutritionally rich medium used to grow various microorganisms, either as a broth or as an agar, with or without added blood?

Escherichia coli, a member of the family Enterobacteriaceae, was resistant to the antibiotic vancomycin.Which of the following statements explains this discrepancy?

An oxidase test was performed on two organisms for quality control purposes.Pseudomonas aeruginosa and Stenotrophomonas maltophilia tested positive and negative, respectively.Which of the following statements explains this discrepancy?

A bacterial colony can be described as:

Reduced O₂ (5% to 10%)and increased CO₂ (8% to 10%)produce an environmental condition that best suits which type of organism?

Which medium can be described as containing hemoglobin, hemin (X factor), and the co-enzyme nicotinamide adenine dinucleotide (NAD)(V factor)?

In which category of media would blood agar best fit?

Which medium can be described as containing bile salts and dyes (e.g., bromothymol blue, acid fuchsin)to slow selectively the growth of most nonpathogenic gram-negative bacilli found in the gastrointestinal tract and allow Salmonella spp.and Shigella spp.to grow?

In which category of media would Löwenstein-Jensen medium best fit?

In which category of media would MacConkey agar best fit?

Which medium can be described as containing crystal violet dye to inhibit the growth of gram-positive bacteria and fungi and allows many types of gram-negative bacilli to grow?

Which category of media can be described as containing some factor or factors that allow colonies of one bacterial species or type to exhibit certain metabolic or culture characteristics that can be used to distinguish them from other bacteria growing on the same agar plate?

Increased concentrations of carbon dioxide (CO₂)(5% to 10%)and approximately 15% oxygen (O₂)produce an environmental condition that best suits which type of organism?

Which category of media can be described as containing specific nutrients required for the growth of particular bacterial pathogens in a patient specimen?

Which of the following organisms grows best at 42º C?

Which category of media can be described as containing nutrients that support the growth of the most nonfastidious organisms without giving any particular organism a growth advantage?

What name is given to the type of hemolysis that produces complete hemolysis and a clearing of the medium?

A catalase test was performed from a sheep blood agar plate on a colony of streptococci, and bubbling was observed.Which of the following statements explains this discrepancy?

What name is given to the type of hemolysis that produces incomplete hemolysis and a greening discoloration of the medium?