Exam 13: Nucleic Acid Biotechnology Techniques

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located. -Refer to Exhibit 13A. If the region between the 2 sites for HgiEII were deleted, the resulting plasmid

Determining disease states using protein arrays may be limited by

Which of the following is not a potential benefit of creating a fusion protein in genetic engineering?

An expression library contains genes corresponding to all of the following, except:

A feature of the Sanger-Coulson method for DNA sequencing is

A palindrome is

Antibiotic-resistance genes in vectors are often used as selectable markers.

Which of the following best describes electrophoretic gels used for DNA?

Recombined bacteriophage particles are easy to isolate, since high concentration of phage will be found in each of the bacterial colonies that grow on the medium used for screening.

A DNA library consists of

The "natural" function of restriction endonucleases is to

The best primers for the PCR reaction have the following feature:

SyBr green and SyBr gold were developed because

Exhibit 13D?The plasmid pSU922 is a circular DNA containing 25000 base pairs. The β-gal gene codes for the enzyme β-galactosidase, the product of which will turn bacterial colonies blue when grown in the presence of X-gal; the Amp^R gene confers ampicillin resistance. -Refer to Exhibit 13D. Neglecting any discussion of whether it's a good or bad choice, I attempt to insert a gene fragment into the HindIII site and transform bacteria with the plasmid. How can I tell which transformants have the insert?

A plasmid is

RNA is sequenced by

The application of gel electrophoresis (PAGE) in DNA sequencing

Sometimes DNA probes are used to prove that a gene has been incorporated into a eukaryotic genome. The following are all steps used in such a procedure, except:

In gene therapy

Cells that contain a "blue/white screening" plasmid that has an added gene are recognized by the method: