Multiple Choice
Exhibit 13D?The plasmid pSU922 is a circular DNA containing 25000 base pairs. The β-gal gene codes for the enzyme β-galactosidase, the product of which will turn bacterial colonies blue when grown in the presence of X-gal; the AmpR gene confers ampicillin resistance.
-Refer to Exhibit 13D. Neglecting any discussion of whether it's a good or bad choice, I attempt to insert a gene fragment into the HindIII site and transform bacteria with the plasmid. How can I tell which transformants have the insert?
A) The bacteria will not be able to grow in the presence of ampicillin, and they will be blue.
B) The bacteria will not be able to grow in the presence of ampicillin, and they will be white.
C) The bacteria will be able to grow in the presence of ampicillin, and they will be blue.
D) The bacteria will be able to grow in the presence of ampicillin, and they will be white.
Correct Answer:
Verified
Correct Answer:
Verified
Q10: The application of gel electrophoresis (PAGE) in
Q29: The best primers for the PCR reaction
Q48: SyBr green and SyBr gold were developed
Q52: Sometimes DNA probes are used to prove
Q66: A plasmid is<br>A) a virus that infects
Q78: The "natural" function of restriction endonucleases is
Q86: In gene therapy<br>A) restriction enzymes are introduced
Q93: Recombined bacteriophage particles are easy to isolate,
Q96: A DNA library consists of<br>A) all the
Q97: RNA is sequenced by<br>A) exactly the same