Exam 13: Nucleic Acid Biotechnology Techniques

Which of the following conditions is not a requirement to assure production of a protein when a plasmid is being designed to express a eukaryotic protein in a bacterium?

A single clone of interest can be distinguished from others in a mixture of clones by

The use of the Bt gene is prohibited in corn plants.

Fluorescent techniques can detect concentrations as low as

If mRNA is converted to DNA, digested with restriction enzymes, and cloned into suitable vectors, the resultant library is called a:

Genetic engineering

While a large majority of people are opposed to somatic gene therapy, a large number support the use of germ-cell gene therapy.

Which of the following are methods by which bacteria can be induced to take up recombinant DNA molecules?

One major obstacle to using bacteria to produce human proteins is because addition of sugar residues to the proteins, glycosylation, never occurs properly.

The polymerase chain reaction requires

The following blotting procedure is named for the scientist who invented it:

In a metabolic study using microarrays, a yellow dot represents the location of DNA on the microarray:

A transcriptome is

Suitable vectors for cloning of recombinant DNA can be

An isoschizomer is a(n)

How does an expression vector differ from a regular cloning vector?

Advantages of the Polymerase Chain Reaction include all of these, except:

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located. -Refer to Exhibit 13A. If a recombinant plasmid were obtained inserting DNA into the BamHI site, the recombinant plasmid would lack which of the following properties?

Microarrays allow the analysis of thousands of samples of DNA or protein to be performed simultaneously.

A chance to be a forensic scientist. RFLP analysis has been requested by the prosecuting attorney in a murder case. DNA samples have been isolated from a blood sample at the crime scene (C), a hair follicle from the victim's hairbrush (V), and a mouth swab of the accused (A). The results of the test are shown. What would you tell the attorney?