Question 1

Which of the following is a unique feature of qPCR compared to the original PCR?

Accepted Answer

A)  qPCR uses a DNA polymerase from a heat stable source 
B)  qPCR requires a primer 
C)  qPCR allows the reaction to run until all of the primers have been exhausted 
D)  In qPCR, the speed with which the DNA is produced is used to estimate how much of the original template was in the reaction vessel 
A)  qPCR uses a DNA polymerase from a heat stable source 
B)  qPCR requires a primer 
C)  qPCR allows the reaction to run until all of the primers have been exhausted 
D)  In qPCR, the speed with which the DNA is produced is used to estimate how much of the original template was in the reaction vessel

Question 2

The following steps are all involved in genetic recombination:
1)Screening for cells that contain the recombined gene.
2)Cutting the vector with restriction enzyme.
3)Mixing the gene of interest with the vector.
4)Isolating the gene of interest from its original source.
5)Ligating the gene of interest and the vector together.
The following sequence of these five steps would be typical:

Accepted Answer

A)  1 → 2 → 3 → 4 → 5 
B)  2 → 3 → 5 → 1 → 4 
C)  5 → 4 → 3 → 2 → 1 
D)  4 → 2 → 3 → 5 → 1 
E)  2 → 3 → 5 → 4 → 1 
A)  1 → 2 → 3 → 4 → 5 
B)  2 → 3 → 5 → 1 → 4 
C)  5 → 4 → 3 → 2 → 1 
D)  4 → 2 → 3 → 5 → 1 
E)  2 → 3 → 5 → 4 → 1

Question 3

In the polymerase chain reaction

Accepted Answer

A)  it is possible to amplify small amounts of DNA without cloning. 
B)  conditions must be carefully controlled to prevent explosions. 
C)  reaction mixtures must be kept chilled at all times. 
D)  all of these 
E)  none of these 
A)  it is possible to amplify small amounts of DNA without cloning. 
B)  conditions must be carefully controlled to prevent explosions. 
C)  reaction mixtures must be kept chilled at all times. 
D)  all of these 
E)  none of these

Question 4

Which of the following was not a technique researchers used to try to improve shelf life of tomatoes with genetic engineering?

Accepted Answer

A)  Insertion of a second polygalactouronase gene 
B)  Using an antisense gene 
C)  Inhibition of the production of ethylene 
D)  Storage in a 40 degree Celcius compartment 
A)  Insertion of a second polygalactouronase gene 
B)  Using an antisense gene 
C)  Inhibition of the production of ethylene 
D)  Storage in a 40 degree Celcius compartment

Question 5

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located.
  
-Refer to Exhibit 13A. If the plasmid were completely digested with the two restriction enzymes BamHI and HgiEII, the resulting fragments would be this large:

Accepted Answer

A)  1920, 1305, 761, and 377 base pairs long. 
B)  1920, 1682 and 761 base pairs long. 
C)  1920, 1680 and 761 base pairs long. 
D)  3056, 2295 and 375 base pairs long. 
E)  The sizes cannot be determined from the data given. 
A)  1920, 1305, 761, and 377 base pairs long. 
B)  1920, 1682 and 761 base pairs long. 
C)  1920, 1680 and 761 base pairs long. 
D)  3056, 2295 and 375 base pairs long. 
E)  The sizes cannot be determined from the data given.

Question 6

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located.
  
-Refer to Exhibit 13A. If a recombinant plasmid were obtained inserting DNA into the AflIII site, the recombinant plasmid would lack which of the following properties?

Accepted Answer

A)  It can replicate autonomously. 
B)  It will be resistant to the antibiotic tetracycline. 
C)  It will be resistant to the antibiotic ampicillin. 
D)  It will possess at lest two promoter sites. 
E)  All of these are true for the recombined plasmid. 
A)  It can replicate autonomously. 
B)  It will be resistant to the antibiotic tetracycline. 
C)  It will be resistant to the antibiotic ampicillin. 
D)  It will possess at lest two promoter sites. 
E)  All of these are true for the recombined plasmid.

Question 7

Which of the following is true?

Accepted Answer

A)  When DNA evidence is used in crime cases, it is easier to prove that a person is guilty than that they are innocent. 
B)  When DNA evidence is used in a paternity case, it is easier to prove that a person is the father rather than the person is not the fathere 
C)  DNA evidence is easier to use to exclude a putative match than to include it 
D)  none of these is true 
A)  When DNA evidence is used in crime cases, it is easier to prove that a person is guilty than that they are innocent. 
B)  When DNA evidence is used in a paternity case, it is easier to prove that a person is the father rather than the person is not the fathere 
C)  DNA evidence is easier to use to exclude a putative match than to include it 
D)  none of these is true

Question 8

The "c" in cDNA stands for this word:

Accepted Answer

A)  Complete. 
B)  Circular. 
C)  Complementary. 
D)  Chromosomal. 
E)  Confusing. 
A)  Complete. 
B)  Circular. 
C)  Complementary. 
D)  Chromosomal. 
E)  Confusing.

Question 9

When fluorescence detection methods are used in biotechnology they

Accepted Answer

A)  have limitations due to low sensitivity 
B)  can be used for only one substance at a time 
C)  are not used in DNA sequencing 
D)  do not present the hazards associated with radioactivity 
A)  have limitations due to low sensitivity 
B)  can be used for only one substance at a time 
C)  are not used in DNA sequencing 
D)  do not present the hazards associated with radioactivity

Question 10

Which of the following statements about plants which contain the recombinant form of the gene from Bacillus thuringiensis (Bt gene), is true?

Accepted Answer

A)  These plants have a natural resistance to caterpillars. 
B)  The gene is expressed in all the plant tissues. 
C)  Since the gene is expressed in the pollen of the plant, there is concern about the gene harming beneficial insects. 
D)  It is likely that some insects will mutate to become resistant to the Bt gene product. 
E)  All of these statements are true. 
A)  These plants have a natural resistance to caterpillars. 
B)  The gene is expressed in all the plant tissues. 
C)  Since the gene is expressed in the pollen of the plant, there is concern about the gene harming beneficial insects. 
D)  It is likely that some insects will mutate to become resistant to the Bt gene product. 
E)  All of these statements are true.

Question 11

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located.
  
-Refer to Exhibit 13A. If a recombinant plasmid were obtained by inserting DNA into the EcoRV site, and the protein corresponding to the recombinant gene were expressed, which of the following statements would be false?

Accepted Answer

A)  The protein will probably contain some extra amino acids on its N-terminal. 
B)  The plasmid will be resistant to ampicillin. 
C)  The plasmid will not be able to replicate autonomously. 
D)  The protein is not likely to be biologically active without some further treatment. 
E)  The question cannot be answered, since it is unlikely that the protein will be expressed at all, because there is no promoter for mRNA synthesis. 
A)  The protein will probably contain some extra amino acids on its N-terminal. 
B)  The plasmid will be resistant to ampicillin. 
C)  The plasmid will not be able to replicate autonomously. 
D)  The protein is not likely to be biologically active without some further treatment. 
E)  The question cannot be answered, since it is unlikely that the protein will be expressed at all, because there is no promoter for mRNA synthesis.

Question 12

Which of the following proteins are produced by recombinant DNA technology but have had their purposes subverted for improving athletic perforrmance?

Accepted Answer

A)  Erythropoietin 
B)  Human Growth Hormone 
C)  Insulin 
D)  Enterokinase 
E)  Erythropoietin and Human Growth Hormone 
F) All of these 
A)  Erythropoietin 
B)  Human Growth Hormone 
C)  Insulin 
D)  Enterokinase 
E)  Erythropoietin and Human Growth Hormone 
F) All of these

Question 13

The presence of bacterial or viral clones is detected experimentally by

Accepted Answer

A)  the presence of colonies or plaques on a suitably prepared Petri dish. 
B)  gel electrophoresis. 
C)  analytical ultracentrifugation. 
D)  x-ray crystallography. 
A)  the presence of colonies or plaques on a suitably prepared Petri dish. 
B)  gel electrophoresis. 
C)  analytical ultracentrifugation. 
D)  x-ray crystallography.

Question 14

Exhibit 13D?The plasmid pSU922 is a circular DNA containing 25000 base pairs. The β-gal gene codes for the enzyme β-galactosidase, the product of which will turn bacterial colonies blue when grown in the presence of X-gal; the AmpS1U1P1RS1S1P0 gene confers ampicillin resistance.
  
-Refer to Exhibit 13D. In three separate vessels, the plasmid is treated with the restriction endonucleases EcoRI, BamHI, and HindIII. Which of the following best represents the electrophoretic gel one would see from these digests?

Accepted Answer

A) 
  
B) 
  
C)  ​
  
D) 
  
A) 
  
B) 
  
C)  ​
  
D)

Which of the following is a unique feature of qPCR compared to the original PCR?

In the polymerase chain reaction

Which of the following was not a technique researchers used to try to improve shelf life of tomatoes with genetic engineering?

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located. -Refer to Exhibit 13A. If the plasmid were completely digested with the two restriction enzymes BamHI and HgiEII, the resulting fragments would be this large:

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located. -Refer to Exhibit 13A. If a recombinant plasmid were obtained inserting DNA into the AflIII site, the recombinant plasmid would lack which of the following properties?

Which of the following is true?

The "c" in cDNA stands for this word:

When fluorescence detection methods are used in biotechnology they

Which of the following statements about plants which contain the recombinant form of the gene from Bacillus thuringiensis (Bt gene), is true?

Which of the following proteins are produced by recombinant DNA technology but have had their purposes subverted for improving athletic perforrmance?

The presence of bacterial or viral clones is detected experimentally by

Biochemistry and the Organization of Cells

Water: the Solvent for Biochemical Reactions

Amino Acids and Peptides

The Three-Dimensional Structure of Proteins

Protein Purification and Characterization Techniques

The Behavior of Proteins: Enzymes

The Behavior of Proteins: Enzymes, Mechanisms, and Control

Lipids and Proteins Are Associated in Biological Membranes

Nucleic Acids: How Structure Conveys Information

Biosynthesis of Nucleic Acids: Replication

Transcription of the Genetic Code: Biosynthesis of RNA

Protein Synthesis: Translation of the Genetic Message

Viruses, Cancer, and Immunology

The Importance of Energy Changes and Electron Transfer in Metabolism

Carbohydrates

Glycolysis

Storage Mechanisms and Control in Carbohydrate Metabolism

The Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Lipid Metabolism

Photosynthesis

The Metabolism of Nitrogen

Integration of Metabolism: Cellular Signaling

Filters

Exam 13: Nucleic Acid Biotechnology Techniques

Which of the following is a unique feature of qPCR compared to the original PCR?

In the polymerase chain reaction

Which of the following was not a technique researchers used to try to improve shelf life of tomatoes with genetic engineering?

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located. -Refer to Exhibit 13A. If the plasmid were completely digested with the two restriction enzymes BamHI and HgiEII, the resulting fragments would be this large:

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located. -Refer to Exhibit 13A. If a recombinant plasmid were obtained inserting DNA into the AflIII site, the recombinant plasmid would lack which of the following properties?

Which of the following is true?

The "c" in cDNA stands for this word:

When fluorescence detection methods are used in biotechnology they

Which of the following statements about plants which contain the recombinant form of the gene from Bacillus thuringiensis (Bt gene), is true?

Which of the following proteins are produced by recombinant DNA technology but have had their purposes subverted for improving athletic perforrmance?

The presence of bacterial or viral clones is detected experimentally by

Biochemistry and the Organization of Cells

Water: the Solvent for Biochemical Reactions

Amino Acids and Peptides

The Three-Dimensional Structure of Proteins

Protein Purification and Characterization Techniques

The Behavior of Proteins: Enzymes

The Behavior of Proteins: Enzymes, Mechanisms, and Control

Lipids and Proteins Are Associated in Biological Membranes

Nucleic Acids: How Structure Conveys Information

Biosynthesis of Nucleic Acids: Replication

Transcription of the Genetic Code: Biosynthesis of RNA

Protein Synthesis: Translation of the Genetic Message

Viruses, Cancer, and Immunology

The Importance of Energy Changes and Electron Transfer in Metabolism

Carbohydrates

Glycolysis

Storage Mechanisms and Control in Carbohydrate Metabolism

The Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Lipid Metabolism

Photosynthesis

The Metabolism of Nitrogen

Integration of Metabolism: Cellular Signaling

Filters