Question 1

Which of the following statements is CORRECT regarding Figure 6.1?

Accepted Answer

A)  Cells are actively growing only during the stage represented by B. 
B)  No cells are reproducing during the stage represented by D. 
C)  Cells are are most susceptible to antibiotics during the stage represented by A. 
D)  Cells are alive but not reproducing during the stage represented by A. 
E)  No new cells are being produced during the stage represented by C. 
A)  Cells are actively growing only during the stage represented by B. 
B)  No cells are reproducing during the stage represented by D. 
C)  Cells are are most susceptible to antibiotics during the stage represented by A. 
D)  Cells are alive but not reproducing during the stage represented by A. 
E)  No new cells are being produced during the stage represented by C.

Question 2

A clinical sample labeled as "sputum" was collected from

Accepted Answer

A)  the skin. 
B)  the blood. 
C)  the central nervous system. 
D)  a piece of tissue. 
E)  the lungs. 
A)  the skin. 
B)  the blood. 
C)  the central nervous system. 
D)  a piece of tissue. 
E)  the lungs.

Question 3

A microorganism grows in the lab on medium containing mineral salts but no organic compounds.The organism is likely to be a(n)

Accepted Answer

A)  heterotroph. 
B)  autotroph. 
C)  chemotroph. 
D)  chemoheterotroph. 
E)  The answer cannot be determined from the information provided. 
A)  heterotroph. 
B)  autotroph. 
C)  chemotroph. 
D)  chemoheterotroph. 
E)  The answer cannot be determined from the information provided.

Question 4

A (Coulter/Petroff-Hauser chamber/spectrophotometer)counter is used to count cells on a light microscope.

Accepted Answer

Petroff-Ha

Question 5

Flow cytometry makes use of fluorescence to count microbes.

Accepted Answer

A) True 
 B)False

Question 6

Explain the similarities and differences between direct and indirect methods of measuring microbial growth,and give examples of each.

Accepted Answer

Direct methods of measuring microbial gr

Question 7

A(n)________ organism require(s)oxygen for growth.

Accepted Answer

A)  anaerobic 
B)  facultative anaerobic 
C)  aerotolerant 
D)  obligate aerobic 
E)  aerotolerant and an anaerobic 
A)  anaerobic 
B)  facultative anaerobic 
C)  aerotolerant 
D)  obligate aerobic 
E)  aerotolerant and an anaerobic

Question 8

A laboratory protocol lists the following ingredients: 1 g sucrose,16.4 g Na2HPO4,1.5 g (NH4)3PO4,0.02 g CaCO3,KNO3,water to 1 liter and autoclave.This recipe is for a ________ medium.

Accepted Answer

A)  defined agar 
B)  defined broth 
C)  complex broth 
D)  reducing agar 
E)  complex enrichment broth 
A)  defined agar 
B)  defined broth 
C)  complex broth 
D)  reducing agar 
E)  complex enrichment broth

Question 9

What types of media are commonly used to isolate anaerobic microbes?

Accepted Answer

A)  defined media 
B)  differential media 
C)  complex media 
D)  reducing media 
E)  selective media 
A)  defined media 
B)  differential media 
C)  complex media 
D)  reducing media 
E)  selective media

Question 10

A microbiologist inoculates a flask of broth with 1 ml of 100 bacterial cells/ml.The generation time of the species is 1 hour,and there is no lag phase.How long will it be before the culture contains more than 6,000 cells/ml?

Accepted Answer

A)  12 hours 
B)  6 hours 
C)  2 hours 
D)  4 hours 
E)  10 hours 
A)  12 hours 
B)  6 hours 
C)  2 hours 
D)  4 hours 
E)  10 hours

Question 11

Compare and contrast the streak-plate method of isolation with the pour-plate method of isolation.

Accepted Answer

The streak-plate and the pour-plate meth

Question 12

In the process of ________,microbes detect the presence and density of other microbes and modify their metabolic activity in response.

Accepted Answer

A)  quorum sensing 
B)  antagonism 
C)  mutualism 
D)  symbiosis 
E)  synergy 
A)  quorum sensing 
B)  antagonism 
C)  mutualism 
D)  symbiosis 
E)  synergy

Question 13

An organism that requires chemical nutrients which serve as final electron acceptors is a(n)

Accepted Answer

A)  chemotroph. 
B)  phototroph. 
C)  lithotroph. 
D)  autotroph. 
E)  aerobe. 
A)  chemotroph. 
B)  phototroph. 
C)  lithotroph. 
D)  autotroph. 
E)  aerobe.

Question 14

Which of the following is an indirect method for estimating the number of microbes in a sample?

Accepted Answer

A)  viable plate counts 
B)  turbidity 
C)  Coulter counter 
D)  MPN 
E)  membrane filtration 
A)  viable plate counts 
B)  turbidity 
C)  Coulter counter 
D)  MPN 
E)  membrane filtration

Question 15

A capnophile is a microorganism that thrives in conditions of high

Accepted Answer

A)  carbon dioxide levels. 
B)  hydrostatic pressure. 
C)  oxygen levels. 
D)  pH. 
E)  salt concentration. 
A)  carbon dioxide levels. 
B)  hydrostatic pressure. 
C)  oxygen levels. 
D)  pH. 
E)  salt concentration.

Question 16

________ are complex communities of various types of microbes that adhere to surfaces.

Accepted Answer

A)  Aggregates 
B)  Colonies 
C)  Isolates 
D)  Biofilms 
E)  Media 
A)  Aggregates 
B)  Colonies 
C)  Isolates 
D)  Biofilms 
E)  Media

Question 17

Microaerophiles are microbes that grow best at low

Accepted Answer

A)  carbon dioxide levels. 
B)  pH values. 
C)  hydrostatic pressure. 
D)  oxygen levels. 
E)  salt concentrations. 
A)  carbon dioxide levels. 
B)  pH values. 
C)  hydrostatic pressure. 
D)  oxygen levels. 
E)  salt concentrations.

Question 18

The Most Probable Number (MPN)method is used

Accepted Answer

A)  to estimate numbers of cells in samples with a high density of bacterial cells. 
B)  when samples contain obligate anaerobes. 
C)  to estimate numbers of cells in samples with a low density of bacterial cells. 
D)  to isolate autotrophic bacteria. 
E)  to determine the generation time of bacterial isolates. 
A)  to estimate numbers of cells in samples with a high density of bacterial cells. 
B)  when samples contain obligate anaerobes. 
C)  to estimate numbers of cells in samples with a low density of bacterial cells. 
D)  to isolate autotrophic bacteria. 
E)  to determine the generation time of bacterial isolates.

Question 19

Serial dilution can be used in combination with pour plates as a method for isolating pure cultures.

Accepted Answer

A) True 
 B)False

Question 20

Which of the following methods provides information about the number of living cells in a sample of bacterial culture?

Accepted Answer

A)  serial dilution and viable plate count 
B)  membrane filtration 
C)  a Petroff-Hauser counting chamber 
D)  flow cytometry 
E)  a Coulter counter 
A)  serial dilution and viable plate count 
B)  membrane filtration 
C)  a Petroff-Hauser counting chamber 
D)  flow cytometry 
E)  a Coulter counter

Exam 6: Microbial Nutrition and Growth

Which of the following statements is CORRECT regarding Figure 6.1?

A clinical sample labeled as "sputum" was collected from

A microorganism grows in the lab on medium containing mineral salts but no organic compounds.The organism is likely to be a(n)

A (Coulter/Petroff-Hauser chamber/spectrophotometer)counter is used to count cells on a light microscope.

Flow cytometry makes use of fluorescence to count microbes.

Explain the similarities and differences between direct and indirect methods of measuring microbial growth,and give examples of each.

A(n)________ organism require(s)oxygen for growth.

A laboratory protocol lists the following ingredients: 1 g sucrose,16.4 g Na2HPO4,1.5 g (NH4)3PO4,0.02 g CaCO3,KNO3,water to 1 liter and autoclave.This recipe is for a ________ medium.

What types of media are commonly used to isolate anaerobic microbes?

A microbiologist inoculates a flask of broth with 1 ml of 100 bacterial cells/ml.The generation time of the species is 1 hour,and there is no lag phase.How long will it be before the culture contains more than 6,000 cells/ml?

Compare and contrast the streak-plate method of isolation with the pour-plate method of isolation.

In the process of ________,microbes detect the presence and density of other microbes and modify their metabolic activity in response.

An organism that requires chemical nutrients which serve as final electron acceptors is a(n)

Which of the following is an indirect method for estimating the number of microbes in a sample?

A capnophile is a microorganism that thrives in conditions of high

________ are complex communities of various types of microbes that adhere to surfaces.

Microaerophiles are microbes that grow best at low

The Most Probable Number (MPN)method is used

Serial dilution can be used in combination with pour plates as a method for isolating pure cultures.

Which of the following methods provides information about the number of living cells in a sample of bacterial culture?

A Brief History of Microbiology

The Chemistry of Microbiology

Cell Structure and Function

Microscopy, Staining, and Classification

Microbial Metabolism

Microbial Genetics

Recombinant DNA Technology

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Body: Antimicrobial Drugs

Characterizing and Classifying Prokaryotes

Characterizing and Classifying Eukaryotes

Characterizing and Classifying Viruses, Viroids, and Prions

Infection, Infectious Diseases, and Epidemiology

Innate Immunity

Adaptive Immunity

Immunization and Immune Testing

Immune Disorders

Pathogenic Gram Positive Bacteria

Pathogenic Gram-Negative Cocci and Bacilli

Rickettsias, Chlamydias, Spirochetes, and Vibrios

Pathogenic Fungi

Parasitic Protozoa, Helminths, and Arthropod Vectors

Pathogenic DNA Viruses

Pathogenic RNA Viruses

Applied and Environmental Microbiology

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