Exam 8: Bacterial Genetics

You are a volunteer for Nurses Without Borders and are being sent on a humanitarian mission. You are somewhat concerned because you have learned from the CDC website that antibiotic-resistant Haemophilus influenzae is common in the country to which you are being sent. This encapsulated, Gram-negative bacterium can cause a variety of illnesses, including community-acquired pneumonia. You do some research on the genetics of this pathogen. -Which of the following statements is CORRECT regarding transformation?

The Ames test determines antibiotic sensitivity of a bacterium.

Not all bacteria can take up DNA from the environment. Those that can are referred to as

The diploid character of eukaryotic cells may mask the appearance of a mutation since

A clever technique that streamlines the identification of auxotrophic mutants is

A patient comes to see you complaining of a spider bite. He shows you a sore on his wrist—the area around it is red and swollen, and the lesion is leaking pus. The patient tells you he found a spider in his car, which is why he thinks this is a bite. He also tells you that he has been taking penicillin tablets that his wife had left over after being prescribed the drug a few months previously for pneumonia. Even though he has taken the tablets for four days, the lesion is not healing and that he is really feeling terrible, with pain, fever, and chills. You suspect that your patient has a wound infection, so you sample the pus and send it to the lab for analysis. -Further tests indicate that your patient has a methicillin-resistant Staphylococcus aureus (MRSA) infection. You explain to the patient that these bacteria are not impacted by penicillin or the related drug methicillin. This resistance is thought to have arisen originally by a random change in the cell's chromosome through a process called 

A mutation in E. coli results in the loss of both restriction endonucleases and modification enzymes. Would you expect any difference in the frequency of gene transfer via transduction FROM Salmonella INTO this E. coli strain?

On which of the following DNA strands would UV radiation have the most effect?

Which of the following statements about spontaneous mutation is True?

A patient comes to see you complaining of a spider bite. He shows you a sore on his wrist—the area around it is red and swollen, and the lesion is leaking pus. The patient tells you he found a spider in his car, which is why he thinks this is a bite. He also tells you that he has been taking penicillin tablets that his wife had left over after being prescribed the drug a few months previously for pneumonia. Even though he has taken the tablets for four days, the lesion is not healing and that he is really feeling terrible, with pain, fever, and chills. You suspect that your patient has a wound infection, so you sample the pus and send it to the lab for analysis. -The lab results indicate that the pus from your patient's "bite" contains clusters of spherical cells that stained purple in the Gram stain. You tell your patient that his wound contains bacteria from the genus ______ and is classified as a ________ organism.

Strong chemical mutagens may be used to treat cancer cells. Is this a good or bad idea?

The material responsible for transformation was shown to be DNA by

Please select the INCORRECT statement regarding mutation.

Transposons may leave a cell by incorporating themselves into a plasmid.

Indirect selection

Crown gall is caused by a prokaryote plasmid that can be expressed in plant cells.

Competent cells

Double-stranded DNA enters the recipient cell during transformation.

Cells that are His⁻, Strᴿ could be isolated from a mixed sample by using a medium containing

Replica plating