Question 1

Multiplex PCR is limited by specific nucleic acid amplification.

Accepted Answer

A) True 
 B)False

Question 2

MATCHING
Match the technique with an appropriate description (use each answer only once).

-Nucleic acid sequence amplification

Accepted Answer

A) This amplifies target nucleic acid without the use of a thermocycler.A double-stranded DNA fragment is created and becomes the target for exponential amplification. 
B) An isothermal assay that targets either DNA or RNA but generates RNA as its amplified product. 
C) Oligonucleotide pairs hybridize to target sequences within the gene or the cryptic plasmid. 
D) Only RNA is targeted for amplification. 
A) This amplifies target nucleic acid without the use of a thermocycler.A double-stranded DNA fragment is created and becomes the target for exponential amplification. 
B) An isothermal assay that targets either DNA or RNA but generates RNA as its amplified product. 
C) Oligonucleotide pairs hybridize to target sequences within the gene or the cryptic plasmid. 
D) Only RNA is targeted for amplification.

Question 3

Match the hybridization technique with the correct description.

-Dot blot

Accepted Answer

A) Hybridization occurs only if the patient's DNA contains base sequences that are 100% complementary to those of the probe. 
B) Both the target nucleic acid and labeled probe interact in solution. 
C) This detects single-base mutations using allele-specific oligonucleotides (ASOs). 
A) Hybridization occurs only if the patient's DNA contains base sequences that are 100% complementary to those of the probe. 
B) Both the target nucleic acid and labeled probe interact in solution. 
C) This detects single-base mutations using allele-specific oligonucleotides (ASOs).

Question 4

MATCHING
Match the technique with an appropriate description (use each answer only once).

-Ligase chain reaction nucleic acid amplification

Accepted Answer

A) This amplifies target nucleic acid without the use of a thermocycler.A double-stranded DNA fragment is created and becomes the target for exponential amplification. 
B) An isothermal assay that targets either DNA or RNA but generates RNA as its amplified product. 
C) Oligonucleotide pairs hybridize to target sequences within the gene or the cryptic plasmid. 
D) Only RNA is targeted for amplification. 
A) This amplifies target nucleic acid without the use of a thermocycler.A double-stranded DNA fragment is created and becomes the target for exponential amplification. 
B) An isothermal assay that targets either DNA or RNA but generates RNA as its amplified product. 
C) Oligonucleotide pairs hybridize to target sequences within the gene or the cryptic plasmid. 
D) Only RNA is targeted for amplification.

Question 5

Clinical outcomes of PCR can lead to:

Accepted Answer

A)  detection of gene mutations that signify the early development of cancer. 
B)  identification of viral DNA associated with specific cancers. 
C)  detection of genetic mutations associated with various diseases. 
D)  all of the above. 
A)  detection of gene mutations that signify the early development of cancer. 
B)  identification of viral DNA associated with specific cancers. 
C)  detection of genetic mutations associated with various diseases. 
D)  all of the above.

Question 6

All the following are true in regard to the polymerase chain reaction (PCR)except:

Accepted Answer

A)  it amplifies low levels of specific DNA sequences. 
B)  the target sequence to be amplified can be unknown. 
C)  two short DNA primers are used. 
D)  oligonucleotides act as a template. 
A)  it amplifies low levels of specific DNA sequences. 
B)  the target sequence to be amplified can be unknown. 
C)  two short DNA primers are used. 
D)  oligonucleotides act as a template.

Question 7

The PCR technique can be modified to study ribonucleic acid (RNA)as well as deoxyribonucleic acid (DNA).

Accepted Answer

A) True 
 B)False

Question 8

Match the hybridization technique with the correct description.

-Liquid-phase hybridization

Accepted Answer

A) Hybridization occurs only if the patient's DNA contains base sequences that are 100% complementary to those of the probe. 
B) Both the target nucleic acid and labeled probe interact in solution. 
C) This detects single-base mutations using allele-specific oligonucleotides (ASOs). 
A) Hybridization occurs only if the patient's DNA contains base sequences that are 100% complementary to those of the probe. 
B) Both the target nucleic acid and labeled probe interact in solution. 
C) This detects single-base mutations using allele-specific oligonucleotides (ASOs).

Question 9

Match the blotting protocol with the clinical application.

-Northern blot

Accepted Answer

A) RNA 
B) DNA 
C) Use of labeled antibodies specific for the protein of interest 
A) RNA 
B) DNA 
C) Use of labeled antibodies specific for the protein of interest

Exam 14: Molecular Techniques

Multiplex PCR is limited by specific nucleic acid amplification.

MATCHING Match the technique with an appropriate description (use each answer only once). -Nucleic acid sequence amplification

Match the hybridization technique with the correct description. -Dot blot

MATCHING Match the technique with an appropriate description (use each answer only once). -Ligase chain reaction nucleic acid amplification

Clinical outcomes of PCR can lead to:

All the following are true in regard to the polymerase chain reaction (PCR)except:

The PCR technique can be modified to study ribonucleic acid (RNA)as well as deoxyribonucleic acid (DNA).

Match the hybridization technique with the correct description. -Liquid-phase hybridization

Match the blotting protocol with the clinical application. -Northern blot

An Overview of Immunology

Antigens and Antibodies

Cells and Cellular Activities of the Immune System: Granulocytes and Mononuclear Cells

Cells and Cellular Activities of the Immune System: Lymphocytes and Plasma Cells

Soluble Mediators of the Immune System

Safety in the Immunology-Serology Laboratory

Quality Assurance and Quality Control

Basic Serologic Laboratory Techniques

Point-Of-Care Testing

Agglutination Methods

Electrophoresis Techniques

Labeling Techniques in Immunoassay

Automated Procedures

The Immune Response in Infectious Diseases

A Primer on Vaccines

Streptococcal Infections

Syphilis

Vector-Borne Diseases

Toxoplasmosis

Cytomegalovirus

Infectious Mononucleosis

Viral Hepatitis

Rubella and Rubeola Infections

Acquired Immunodeficiency Syndrome

Hypersensitivity Reactions

Immunoproliferative Disorders

Autoimmune Disorders

Systemic Lupus Erythematosus

Rheumatoid Arthritis

Solid Organ Transplantation

Bone Marrow Transplantation

Tumor Immunology

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