Question 1

Complete the final dilution row (1,2,3)in the table below using the following dilutions:
 
A)1:3
B)1:4
C)1:64
D)1:256

Accepted Answer

1,B;2,C;3,D
Example of Preparation of a Serial Dilution

Question 2

Automatic pipettes have the advantage of:

Accepted Answer

A)  being fast. 
B)  allowing repetitive measurement of solutions. 
C)  delivering equal volumes of solutions. 
D)  all of the above. 
A)  being fast. 
B)  allowing repetitive measurement of solutions. 
C)  delivering equal volumes of solutions. 
D)  all of the above. D

Question 3

Match the term with the appropriate description.
-Serologic pipette

Accepted Answer

A) Delivers the amount of liquid contained between two calibration marks on the pipette. 
B) The letters TD (to deliver)appear on the pipette. 
A) Delivers the amount of liquid contained between two calibration marks on the pipette. 
B) The letters TD (to deliver)appear on the pipette. B

Question 4

A dilution is a(n):

Accepted Answer

A)  ratio of volume or number of parts of the substance to be diluted in the total volume,or parts,of the final solution. 
B)  indication of relative concentration. 
C)  frequently used measure in serologic testing. 
D)  all of the above. 
A)  ratio of volume or number of parts of the substance to be diluted in the total volume,or parts,of the final solution. 
B)  indication of relative concentration. 
C)  frequently used measure in serologic testing. 
D)  all of the above.

Question 5

Match the term with the appropriate description.
-Graduated pipette

Accepted Answer

A) Delivers the amount of liquid contained between two calibration marks on the pipette. 
B) The letters TD (to deliver)appear on the pipette. 
A) Delivers the amount of liquid contained between two calibration marks on the pipette. 
B) The letters TD (to deliver)appear on the pipette.

Question 6

MATCHING
Match the term with the appropriate definition.

-Hemolysis

Accepted Answer

A) Rupturing of red blood cells that produces a pink or reddish color to serum or plasma 
B) Emulsion of fat globules that often appears in serum after eating,during digestion 
C) Yellowish color to plasma or serum 
A) Rupturing of red blood cells that produces a pink or reddish color to serum or plasma 
B) Emulsion of fat globules that often appears in serum after eating,during digestion 
C) Yellowish color to plasma or serum

Question 7

A central concept of serologic testing is:

Accepted Answer

A)  antigen-antibody interaction. 
B)  determination of antibody composition. 
C)  determination of antigen titer. 
D)  rise in antibody titer. 
A)  antigen-antibody interaction. 
B)  determination of antibody composition. 
C)  determination of antigen titer. 
D)  rise in antibody titer.

Question 8

MATCHING
Match the term with the appropriate definition.

-Icteric

Accepted Answer

A) Rupturing of red blood cells that produces a pink or reddish color to serum or plasma 
B) Emulsion of fat globules that often appears in serum after eating,during digestion 
C) Yellowish color to plasma or serum 
A) Rupturing of red blood cells that produces a pink or reddish color to serum or plasma 
B) Emulsion of fat globules that often appears in serum after eating,during digestion 
C) Yellowish color to plasma or serum

Question 9

A meniscus is the:

Accepted Answer

A)  curvature in the top surface of a liquid. 
B)  zero mark on a pipette. 
C)  last marking on a serologic pipette. 
D)  flat line of liquid in a pipette. 
A)  curvature in the top surface of a liquid. 
B)  zero mark on a pipette. 
C)  last marking on a serologic pipette. 
D)  flat line of liquid in a pipette.

Question 10

MATCHING
Match the term with the appropriate definition.

-Chyle

Accepted Answer

A) Rupturing of red blood cells that produces a pink or reddish color to serum or plasma 
B) Emulsion of fat globules that often appears in serum after eating,during digestion 
C) Yellowish color to plasma or serum 
A) Rupturing of red blood cells that produces a pink or reddish color to serum or plasma 
B) Emulsion of fat globules that often appears in serum after eating,during digestion 
C) Yellowish color to plasma or serum

Question 11

A graduated pipette can be used when:

Accepted Answer

A)  extreme accuracy is needed. 
B)  very precise accuracy is needed. 
C)  great accuracy is not needed. 
D)  speed is more important than precision. 
A)  extreme accuracy is needed. 
B)  very precise accuracy is needed. 
C)  great accuracy is not needed. 
D)  speed is more important than precision.

Question 12

To dilute a serum specimen for a 1:10 dilution,

-parts of distilled water would be added to the 10-mL mark.

Accepted Answer

A) 0.01 
B) 0.1 
C) 0.5 
D) 1.0 
E) 10
F)9
G)4.5
H)0.10 
A) 0.01 
B) 0.1 
C) 0.5 
D) 1.0 
E) 10
F)9
G)4.5
H)0.10

Question 13

Serum for the detection of antibodies should be drawn during the:

Accepted Answer

A)  acute phase of illness only. 
B)  acute and convalescent phases of illness. 
C)  convalescent phase of illness only. 
D)  acute and convalescent phases,as well as 6 months after an illness. 
A)  acute phase of illness only. 
B)  acute and convalescent phases of illness. 
C)  convalescent phase of illness only. 
D)  acute and convalescent phases,as well as 6 months after an illness.

Question 14

Complement can be inactivated in human serum by heating to _____° C.

Accepted Answer

A)  25 
B)  37 
C)  45 
D)  56 
A)  25 
B)  37 
C)  45 
D)  56

Question 15

The term antibody titer is defined as:

Accepted Answer

A)  the reciprocal of the highest dilution of the patient's serum. 
B)  a change in antibody strength. 
C)  the response to an antigen. 
D)  both b and c 
A)  the reciprocal of the highest dilution of the patient's serum. 
B)  a change in antibody strength. 
C)  the response to an antigen. 
D)  both b and c

Question 16

A specimen should be reinactivated when more than ______ hour(s)has (have)elapsed since inactivation.

Accepted Answer

A)  1 
B)  2 
C)  4 
D)  8 
A)  1 
B)  2 
C)  4 
D)  8

Question 17

To dilute a serum specimen for a 1:10 dilution,

-parts of serum would be added to a tube,and then

Accepted Answer

A) 0.01 
B) 0.1 
C) 0.5 
D) 1.0 
E) 10
F)9
G)4.5
H)0.10 
A) 0.01 
B) 0.1 
C) 0.5 
D) 1.0 
E) 10
F)9
G)4.5
H)0.10

Question 18

Factors that can denature,coagulate,or alter protein molecules include:

Accepted Answer

A)  heat. 
B)  bacteria. 
C)  diluting the serum specimen. 
D)  both a and b 
A)  heat. 
B)  bacteria. 
C)  diluting the serum specimen. 
D)  both a and b

Exam 8: Basic Serologic Laboratory Techniques

Complete the final dilution row (1,2,3)in the table below using the following dilutions: A)1:3 B)1:4 C)1:64 D)1:256

Automatic pipettes have the advantage of:

Match the term with the appropriate description. -Serologic pipette

A dilution is a(n):

Match the term with the appropriate description. -Graduated pipette

MATCHING Match the term with the appropriate definition. -Hemolysis

A central concept of serologic testing is:

MATCHING Match the term with the appropriate definition. -Icteric

A meniscus is the:

MATCHING Match the term with the appropriate definition. -Chyle

A graduated pipette can be used when:

To dilute a serum specimen for a 1:10 dilution, -parts of distilled water would be added to the 10-mL mark.

Serum for the detection of antibodies should be drawn during the:

Complement can be inactivated in human serum by heating to _____° C.

The term antibody titer is defined as:

A specimen should be reinactivated when more than ______ hour(s)has (have)elapsed since inactivation.

To dilute a serum specimen for a 1:10 dilution, -parts of serum would be added to a tube,and then

Factors that can denature,coagulate,or alter protein molecules include:

An Overview of Immunology

Antigens and Antibodies

Cells and Cellular Activities of the Immune System: Granulocytes and Mononuclear Cells

Cells and Cellular Activities of the Immune System: Lymphocytes and Plasma Cells

Soluble Mediators of the Immune System

Safety in the Immunology-Serology Laboratory

Quality Assurance and Quality Control

Point-Of-Care Testing

Agglutination Methods

Electrophoresis Techniques

Labeling Techniques in Immunoassay

Automated Procedures

Molecular Techniques

The Immune Response in Infectious Diseases

A Primer on Vaccines

Streptococcal Infections

Syphilis

Vector-Borne Diseases

Toxoplasmosis

Cytomegalovirus

Infectious Mononucleosis

Viral Hepatitis

Rubella and Rubeola Infections

Acquired Immunodeficiency Syndrome

Hypersensitivity Reactions

Immunoproliferative Disorders

Autoimmune Disorders

Systemic Lupus Erythematosus

Rheumatoid Arthritis

Solid Organ Transplantation

Bone Marrow Transplantation

Tumor Immunology

Filters