Question 1

In fluorescent antibody microscopy using a fluorescent substance or dye,the incident light is infrared.

Accepted Answer

A) True 
 B)False

Question 2

Match the following emerging technology with the appropriate definition.

-SQUID technology

Accepted Answer

A) Semiconductor nanocrystals 
B) Method of tagging antibodies with superparamagnetic particles 
C) Technology based on two different 200-nm latex particles 
D) Molecular cytogenetic technique 
E) May be used in many fluorescent and colorimetric applications 
A) Semiconductor nanocrystals 
B) Method of tagging antibodies with superparamagnetic particles 
C) Technology based on two different 200-nm latex particles 
D) Molecular cytogenetic technique 
E) May be used in many fluorescent and colorimetric applications

Question 3

Indirect immunofluorescent assay (IFA)is widely used for the detection of:

Accepted Answer

A)  autoantibodies. 
B)  antibody to tissue antigen. 
C)  antibody to cellular antigen. 
D)  all of the above. 
A)  autoantibodies. 
B)  antibody to tissue antigen. 
C)  antibody to cellular antigen. 
D)  all of the above.

Question 4

Match the type of enzyme immunoassay (EIA)with the appropriate description.
-Capture EIA

Accepted Answer

A) The amount of color that develops in the reaction is inversely proportional to the amount of antibody. 
B) Antibody specific for immunoglobulin M (IgM)or IgG is attached to a solid-phase surface. 
C) A specific antigen is attached to a solid-phase surface. 
A) The amount of color that develops in the reaction is inversely proportional to the amount of antibody. 
B) Antibody specific for immunoglobulin M (IgM)or IgG is attached to a solid-phase surface. 
C) A specific antigen is attached to a solid-phase surface.

Question 5

Match the following emerging technology with the appropriate definition.

-Signal amplification technology

Accepted Answer

A) Semiconductor nanocrystals 
B) Method of tagging antibodies with superparamagnetic particles 
C) Technology based on two different 200-nm latex particles 
D) Molecular cytogenetic technique 
E) May be used in many fluorescent and colorimetric applications 
A) Semiconductor nanocrystals 
B) Method of tagging antibodies with superparamagnetic particles 
C) Technology based on two different 200-nm latex particles 
D) Molecular cytogenetic technique 
E) May be used in many fluorescent and colorimetric applications

Question 6

Fluorescent molecules in immunofluorescent techniques are used as substitutes for:

Accepted Answer

A)  radioisotopes. 
B)  enzyme labels. 
C)  antibody substitutes. 
D)  both a and b 
A)  radioisotopes. 
B)  enzyme labels. 
C)  antibody substitutes. 
D)  both a and b

Question 7

FISH technology:

Accepted Answer

A)  uses recombinant DNA technology. 
B)  detects hematopathologic chromosomal abnormalities. 
C)  detects oncogenic chromosomal abnormalities. 
D)  all of the above. 
A)  uses recombinant DNA technology. 
B)  detects hematopathologic chromosomal abnormalities. 
C)  detects oncogenic chromosomal abnormalities. 
D)  all of the above.

Question 8

Match the assays and definitions.

-Direct immunofluorescent assay

Accepted Answer

A) Antibodies can act as antigens and react with antiimmunoglobulins. 
B) Conjugated antibody is used to detect antigen-antibody reactions at a microscopic level. 
C) An antigen is first exposed to unlabeled antibody and then to labeled antibody. 
A) Antibodies can act as antigens and react with antiimmunoglobulins. 
B) Conjugated antibody is used to detect antigen-antibody reactions at a microscopic level. 
C) An antigen is first exposed to unlabeled antibody and then to labeled antibody.

Exam 12: Labeling Techniques in Immunoassay

In fluorescent antibody microscopy using a fluorescent substance or dye,the incident light is infrared.

Match the following emerging technology with the appropriate definition. -SQUID technology

Indirect immunofluorescent assay (IFA)is widely used for the detection of:

Match the type of enzyme immunoassay (EIA)with the appropriate description. -Capture EIA

Match the following emerging technology with the appropriate definition. -Signal amplification technology

Fluorescent molecules in immunofluorescent techniques are used as substitutes for:

FISH technology:

Match the assays and definitions. -Direct immunofluorescent assay

An Overview of Immunology

Antigens and Antibodies

Cells and Cellular Activities of the Immune System: Granulocytes and Mononuclear Cells

Cells and Cellular Activities of the Immune System: Lymphocytes and Plasma Cells

Soluble Mediators of the Immune System

Safety in the Immunology-Serology Laboratory

Quality Assurance and Quality Control

Basic Serologic Laboratory Techniques

Point-Of-Care Testing

Agglutination Methods

Electrophoresis Techniques

Automated Procedures

Molecular Techniques

The Immune Response in Infectious Diseases

A Primer on Vaccines

Streptococcal Infections

Syphilis

Vector-Borne Diseases

Toxoplasmosis

Cytomegalovirus

Infectious Mononucleosis

Viral Hepatitis

Rubella and Rubeola Infections

Acquired Immunodeficiency Syndrome

Hypersensitivity Reactions

Immunoproliferative Disorders

Autoimmune Disorders

Systemic Lupus Erythematosus

Rheumatoid Arthritis

Solid Organ Transplantation

Bone Marrow Transplantation

Tumor Immunology

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