Exam 9: What Genes Are

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When looking at a single-stranded piece of DNA,

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The polymerase chain reaction (PCR)technique could be developed only after the discovery of bacterial enzymes that were able to withstand high temperatures.Why was it necessary to use such enzymes?

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Viral DNA that has entered the pig genome and remains active is passed from generation to generation through

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Are mutations always harmful? Justify your answer using an example.

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Targeting the pol gene in pig DNA works to remove the 62 porcine endogenous retrovirus (PERV)genes because the pol gene is typically ________ in all 62 copies of PERV genes and ________ in other pig genes.

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If a segment of double-stranded DNA has 20 percent adenine (A),how much of it is cytosine (C)?

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The significance of specific base pairing in DNA is that

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When examining the two complementary strands of nucleotides in one DNA molecule,it would be expected that the sum of ________ in one strand would be ________ the sum of the ________ in the other strand.

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The order of the base pairs in a DNA molecule

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Put the following in order from smallest to largest. -This is the largest.

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Which of the following must occur for a mismatch error to be repaired?

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In the first step of the polymerase chain reaction (PCR),the two strands of the DNA being amplified must be separated by exposing the DNA to high ________.

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Compare the segment of DNA for normal hemoglobin DNA with the segment for sickle-cell hemoglobin DNA below.Sickle-cell disease is caused by a ________ mutation. Compare the segment of DNA for normal hemoglobin DNA with the segment for sickle-cell hemoglobin DNA below.Sickle-cell disease is caused by a ________ mutation.

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When studying genomes of organisms,scientists often will only list the sequence of a single strand.Explain why the sequence of the second strand would be unnecessary information.

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The polymerase chain reaction (PCR)is used to

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The difference between the alleles of a single gene is

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The CRISPR-Cas9 system is used to generate a ________ mutation when most of the pol gene is "knocked out" and any remnants of the pol gene are nonfunctional.

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It could help meet transplant needs to use a patient's own stem cells to grow a human liver in a pig embryo modified by CRISPR.As an additional and essential step in the process to ensure the liver is safe for the patient,CRISPR should be used to

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A scientist uses polymerase chain reaction (PCR)to compare the sequence of a disease gene and a healthy gene.The scientist finds that these two genes are identical except for one G in place of a T at position 256 in the gene.This type of mutation is a(n)

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If the human transplant patient in the figure below needs a new kidney because of a genetic mutation that has made her own kidneys nonfunctional,what additional step would need to happen to produce a functional kidney for this patient? If the human transplant patient in the figure below needs a new kidney because of a genetic mutation that has made her own kidneys nonfunctional,what additional step would need to happen to produce a functional kidney for this patient?

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