Question 1

Which of the following is a part of the eukaryotic deadenylation process?

Accepted Answer

A) adenylation of circularized mRNA 
B) an increase in binding sites for PABP 
C) mRNA linearization once the poly(
A) tail is down to approximately 80 nucleotides 
D) decreased interaction between PABP and eIF4G 
A) adenylation of circularized mRNA 
B) an increase in binding sites for PABP 
C) mRNA linearization once the poly(
A) tail is down to approximately 80 nucleotides 
D) decreased interaction between PABP and eIF4G

Question 2

Which of the following is NOT a protein component of P-bodies?

Accepted Answer

A) Edc3p: decapping activator 
B) Argonautes: miRNA silencing 
C) CPEB: cytoplasmic polyadenylation element-binding protein 
D) eIF2: eukaryotic initiation factor 
A) Edc3p: decapping activator 
B) Argonautes: miRNA silencing 
C) CPEB: cytoplasmic polyadenylation element-binding protein 
D) eIF2: eukaryotic initiation factor

Question 3

Which of the following is a characteristic of P-bodies?

Accepted Answer

A) They are mitochondrial foci consisting of RNA-protein complexes. 
B) Under stress conditions they typically reduce their sizes. 
C) They are aggregates of messenger ribonucleoproteins (mRNPs)and P-body components. 
D) They are aggregates of messenger ribonucleoproteins (mRNPs)and P-body components 
A) They are mitochondrial foci consisting of RNA-protein complexes. 
B) Under stress conditions they typically reduce their sizes. 
C) They are aggregates of messenger ribonucleoproteins (mRNPs)and P-body components. 
D) They are aggregates of messenger ribonucleoproteins (mRNPs)and P-body components

Question 4

How do mammalian cells distinguish between PTCs and proper stop codons?

Accepted Answer

A) using AREs 
B) using RNP1 and RNP2 
C) using the EJC 
D) using GMD 
A) using AREs 
B) using RNP1 and RNP2 
C) using the EJC 
D) using GMD

Question 5

Which of the following is a characteristic of ARE-containing mRNAs?

Accepted Answer

A) Class II AREs consist of at least 2 overlapping copies of UUAUUUA(U/
A) (U/
A) . 
B) Class I AREs contain 1-3 copies of the pentanucleotide AUUUA with only A-rich regions. 
C) Class III AREs contain a U-rich sequence with the typical AUUUA element. 
D) Approximately 18% of analyzed human mRNA sequences belong to the ARE-containing family. 
A) Class II AREs consist of at least 2 overlapping copies of UUAUUUA(U/
A) (U/
A) . 
B) Class I AREs contain 1-3 copies of the pentanucleotide AUUUA with only A-rich regions. 
C) Class III AREs contain a U-rich sequence with the typical AUUUA element. 
D) Approximately 18% of analyzed human mRNA sequences belong to the ARE-containing family.

Question 6

Which of the following components protects mRNAs from cellular nucleases?

Accepted Answer

A) 5'-GTP cap 
B) introns 
C) phosphorylation 
D) TATA box 
A) 5'-GTP cap 
B) introns 
C) phosphorylation 
D) TATA box

Question 7

Which of the following is GRE-mediated mRNA decay (GMD)dependent upon?

Accepted Answer

A) RNP1 
B) ARE 
C) AUBP 
D) CUGBP1 
A) RNP1 
B) ARE 
C) AUBP 
D) CUGBP1

Question 8

Which of the following is NOT stored in P-bodies?

Accepted Answer

A) CCR4-NOT 
B) DCP1/DCP2 
C) repressed mRNAs 
D) AREs 
A) CCR4-NOT 
B) DCP1/DCP2 
C) repressed mRNAs 
D) AREs

Question 9

What does the -55 boundary rule signify with respect to NMD?

Accepted Answer

A) Only stop codons at least 55 nt downstream of the final intron trigger NMD. 
B) Only stop codons at least 55 nt upstream of the final intron trigger NMD. 
C) Only stop codons at least 55 nt downstream of the first intron trigger NMD. 
D) Only stop codons at least 55 nt upstream of the first intron trigger NMD. 
A) Only stop codons at least 55 nt downstream of the final intron trigger NMD. 
B) Only stop codons at least 55 nt upstream of the final intron trigger NMD. 
C) Only stop codons at least 55 nt downstream of the first intron trigger NMD. 
D) Only stop codons at least 55 nt upstream of the first intron trigger NMD.

Question 10

Which of the following best describes the term mRNA half-life?

Accepted Answer

A) the length of time an mRNA molecule takes to degrade 
B) the length of time an mRNA molecule takes to be modified 
C) the length of time an mRNA molecule is stable inside the cell 
D) the length of time an mRNA molecule takes to be synthesized 
A) the length of time an mRNA molecule takes to degrade 
B) the length of time an mRNA molecule takes to be modified 
C) the length of time an mRNA molecule is stable inside the cell 
D) the length of time an mRNA molecule takes to be synthesized

Question 11

Which of the following repeating nucleotide motifs are contained in AREs?

Accepted Answer

A) UAAAU 
B) AUAUA 
C) UAUAU 
D) AUUUA 
A) UAAAU 
B) AUAUA 
C) UAUAU 
D) AUUUA

Question 12

Which of the following techniques makes use of photoactivatable ribonucleosides?

Accepted Answer

A) PAR-CLIP 
B) RIP-Chip 
C) RNA-EMSA 
D) RBP-mRNA-PCR 
A) PAR-CLIP 
B) RIP-Chip 
C) RNA-EMSA 
D) RBP-mRNA-PCR

Question 13

Which of the following cleaves the pre-miRNA at the level of the loop,yielding a 22-nt,double-stranded RNA molecule?

Accepted Answer

A) Drosha 
B) DGCR8 
C) Dicer 
D) RISC 
A) Drosha 
B) DGCR8 
C) Dicer 
D) RISC

Question 14

Which of the following best defines miRNAs?

Accepted Answer

A) non-coding RNAs that modulate the transcription of mRNA 
B) non-coding RNAs that modulate the translation of mRNA 
C) coding RNAs that modulate the transcription of mRNA 
D) coding RNAs that modulate the translation of mRNA 
A) non-coding RNAs that modulate the transcription of mRNA 
B) non-coding RNAs that modulate the translation of mRNA 
C) coding RNAs that modulate the transcription of mRNA 
D) coding RNAs that modulate the translation of mRNA

Question 15

Which of the following techniques can be used to identify RBP-mRNA interactions?

Accepted Answer

A) SDS-PAGE 
B) Southern blot analysis 
C) RNA-EMSA 
D) RT-PCR 
A) SDS-PAGE 
B) Southern blot analysis 
C) RNA-EMSA 
D) RT-PCR

Question 16

Which of the following is an example of a Class II ARE-containing mRNA?

Accepted Answer

A) c-myc 
B) TNFα 
C) GLUT1 
D) c-fos 
A) c-myc 
B) TNFα 
C) GLUT1 
D) c-fos

Question 17

Which of the following is NOT used to measure the decay of an mRNA transcript?

Accepted Answer

A) actinomycin D 
B) α-amanitin 
C) 5,6-dichloro-1-β-D-ribobenzimidazole (DR
B)  
D) puromycin 
A) actinomycin D 
B) α-amanitin 
C) 5,6-dichloro-1-β-D-ribobenzimidazole (DR
B)  
D) puromycin

Question 18

Shortening of the poly(A)tail is mediated by enzyme complexes known as deadenylases.Which of the following is NOT a eukaryotic deadenylase?

Accepted Answer

A) UTR 
B) PAN₂-PAN3 
C) CCR4-NOT 
D) PARN 
A) UTR 
B) PAN₂-PAN3 
C) CCR4-NOT 
D) PARN

Exam 31: Post-Transcriptional Regulation of Gene Expression

Which of the following is a part of the eukaryotic deadenylation process?

Which of the following is NOT a protein component of P-bodies?

Which of the following is a characteristic of P-bodies?

How do mammalian cells distinguish between PTCs and proper stop codons?

Which of the following is a characteristic of ARE-containing mRNAs?

Which of the following components protects mRNAs from cellular nucleases?

Which of the following is GRE-mediated mRNA decay (GMD)dependent upon?

Which of the following is NOT stored in P-bodies?

What does the -55 boundary rule signify with respect to NMD?

Which of the following best describes the term mRNA half-life?

Which of the following repeating nucleotide motifs are contained in AREs?

Which of the following techniques makes use of photoactivatable ribonucleosides?

Which of the following cleaves the pre-miRNA at the level of the loop,yielding a 22-nt,double-stranded RNA molecule?

Which of the following best defines miRNAs?

Which of the following techniques can be used to identify RBP-mRNA interactions?

Which of the following is an example of a Class II ARE-containing mRNA?

Which of the following is NOT used to measure the decay of an mRNA transcript?

Shortening of the poly(A)tail is mediated by enzyme complexes known as deadenylases.Which of the following is NOT a eukaryotic deadenylase?

The Facts of Life: Chemistry Is the Logic of Biological Phenomena

Water: the Medium of Life

Thermodynamics of Biological Systems

Amino Acids

Proteins: Their Primary Structure and Biological Functions

Proteins: Secondary, tertiary, and Quaternary Structure

Carbohydrates and the Glycoconjugates of Cell Surfaces

Lipids

Lipid Biosynthesis

Membranes and Membrane Transport

The Reception and Transmission of Extracellular Information

Enzymes Kinetics and Specificity

Mechanisms of Enzyme Action

Enzyme Regulation

Molecular Motors

Nutrition and the Organization of Metabolism

Glycolysis

The Tricarboxylic Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Gluconeogenesis, glycogen Metabolism, and the Pentose Phosphate Pathway

Fatty Acid Catabolism

Nitrogen Acquisition and Amino Acid Metabolism

Nucleotides and Nucleic Acids

Structure of Nucleic Acids

Synthesis and Degradation of Nucleotides

Recombinant Dna: Cloning and Creation of Chimeric Genes

DNA Metabolism: Replication, recombination, and Repair

Transcription and the Regulation of Gene Expression

Protein Synthesis

Completing the Protein Life Cycle: Folding, processing, and Degradation

Metabolic Integration and Organ Specialization

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