Question 1

Which of the following is needed for an inducible operon to be transcribed?

Accepted Answer

A) presence of a small molecule co-inducer 
B) absence of a small molecule co-inducer 
C) presence of a protein inducer 
D) absence of a protein inducer 
A) presence of a small molecule co-inducer 
B) absence of a small molecule co-inducer 
C) presence of a protein inducer 
D) absence of a protein inducer

Question 2

Which of the following occurs when the heptapeptide repeat in the carboxy terminal domain in the RPB1 subunit of RNA polymerase II is phosphorylated?

Accepted Answer

A) conversion to an elongation complex 
B) binding to DNA 
C) formation of a holo-RNA polymerase II 
D) initiation of transcription 
A) conversion to an elongation complex 
B) binding to DNA 
C) formation of a holo-RNA polymerase II 
D) initiation of transcription

Question 3

Which of the following recurring structural features is found in DNA-binding proteins?

Accepted Answer

A) α-helix 
B) β-sheet 
C) β-turn 
D) β-barrel 
A) α-helix 
B) β-sheet 
C) β-turn 
D) β-barrel

Question 4

The stand of dsDNA that is read by RNA polymerase is termed the template strand with RNA polymerase moving 3'?5' along it.Which of the following does the polymerase form,and in which direction does it grow?

Accepted Answer

A) transcript;3'?5' 
B) transcript;5'?3' 
C) template;3'?5' 
D) template;5'?3' 
A) transcript;3'?5' 
B) transcript;5'?3' 
C) template;3'?5' 
D) template;5'?3'

Question 5

Which of the following is NOT classified as a spliceosome?

Accepted Answer

A) snRNP 
B) RNA-annealing protein 
C) ATP-dependent RNA-unwinding protein 
D) enzyme catalyzing hydrolysis of phosphoester 
A) snRNP 
B) RNA-annealing protein 
C) ATP-dependent RNA-unwinding protein 
D) enzyme catalyzing hydrolysis of phosphoester

Question 6

Which of the following is NOT a stage in transcription?

Accepted Answer

A) binding of RNA polymerase holoenzyme at the promoter sites 
B) chain elongation 
C) DNase I activity on RNA polymerase/DNA complex 
D) initiation of polymerization 
A) binding of RNA polymerase holoenzyme at the promoter sites 
B) chain elongation 
C) DNase I activity on RNA polymerase/DNA complex 
D) initiation of polymerization

Question 7

Heat shock element (HSE)is a response element found in the promoter region of genes.How is transcription of the HSE activated?

Accepted Answer

A) in response to cold 
B) in response to elevated temperature 
C) in response to sunlight 
D) in response to darkness 
A) in response to cold 
B) in response to elevated temperature 
C) in response to sunlight 
D) in response to darkness

Question 8

The initiation site binds a purine nucleotide base pairing with the +1 base exposed within the open promoter complex.Which of the following is where the second base is added?

Accepted Answer

A) to the 5'-C of the first base 
B) to the 3'-C of the first base 
C) to the 5'-O of the first base 
D) to the 3'-O of the first base 
A) to the 5'-C of the first base 
B) to the 3'-C of the first base 
C) to the 5'-O of the first base 
D) to the 3'-O of the first base

Question 9

There are 2 consensus sequence elements within the promoter sequence.Which of the following are they?

Accepted Answer

A) the rho subunit near -10 with a sequence of TATAAT;the sigma subunit containing the consensus TTGACA 
B) the Pribnow box near -10 with a sequence of TATAAT;the sigma subunit containing the consensus TTGACA 
C) the rho subunit near -10 with a sequence of TATAAT;the Pribnow box containing the consensus TTGACA 
D) the Pribnow box near -10 with a sequence of TATAAT;the -35 region containing the consensus TTGACA 
A) the rho subunit near -10 with a sequence of TATAAT;the sigma subunit containing the consensus TTGACA 
B) the Pribnow box near -10 with a sequence of TATAAT;the sigma subunit containing the consensus TTGACA 
C) the rho subunit near -10 with a sequence of TATAAT;the Pribnow box containing the consensus TTGACA 
D) the Pribnow box near -10 with a sequence of TATAAT;the -35 region containing the consensus TTGACA

Question 10

Which of the following is thought to be generated by transcription chain elongation?

Accepted Answer

A) positive supercoils behind the transcription bubble 
B) positive supercoils in front of the transcription bubble 
C) negative supercoils in front of the transcription bubble 
D) a relaxed DNA during the transcription process 
A) positive supercoils behind the transcription bubble 
B) positive supercoils in front of the transcription bubble 
C) negative supercoils in front of the transcription bubble 
D) a relaxed DNA during the transcription process

Question 11

Which of the following is a characteristic of protein isoforms?

Accepted Answer

A) They are related polypeptides. 
B) They have identical structure and function. 
C) They are the result of different transcripts of a single gene. 
D) They are mirror images. 
A) They are related polypeptides. 
B) They have identical structure and function. 
C) They are the result of different transcripts of a single gene. 
D) They are mirror images.

Question 12

Which of the following is a characteristic of the Zn-finger motif?

Accepted Answer

A) It binds the minor groove of DNA. 
B) There is a repeated motif in DNA-binding proteins. 
C) Two Cys and 2 Met coordinate the Zn. 
D) It interacts with about 2 nucleotides/Zn finger. 
A) It binds the minor groove of DNA. 
B) There is a repeated motif in DNA-binding proteins. 
C) Two Cys and 2 Met coordinate the Zn. 
D) It interacts with about 2 nucleotides/Zn finger.

Question 13

Which of the following is NOT a control mechanism that regulates transcription in prokaryotes?

Accepted Answer

A) protein:fatty acid interactions 
B) DNA:protein interactions 
C) RNA:protein interactions 
D) fatty acid:cholesterol interactions 
A) protein:fatty acid interactions 
B) DNA:protein interactions 
C) RNA:protein interactions 
D) fatty acid:cholesterol interactions

Question 14

Which of the following is where are nucleotides said to lie relative to the transcription site when they are in the "minus" direction?

Accepted Answer

A) downstream 
B) upstream 
C) downstrand 
D) upstrand 
A) downstream 
B) upstream 
C) downstrand 
D) upstrand

Question 15

Which of the following eukaryotic transcription factors exhibits sequence specificity for the TATA box?

Accepted Answer

A) TFIIB 
B) TFIIC 
C) TFIID/TBP 
D) TFIIH 
A) TFIIB 
B) TFIIC 
C) TFIID/TBP 
D) TFIIH

Question 16

Which of the following DNA-binding motifs common to many prokaryotic regulatory proteins is the lac repressor an example of?

Accepted Answer

A) a homeobox 
B) a zinc finger 
C) a basic region-leucine finger 
D) a helix-turn-helix 
A) a homeobox 
B) a zinc finger 
C) a basic region-leucine finger 
D) a helix-turn-helix

Question 17

Which of the following terms defines the huge multi-subunit entities that mediate ATP-dependent conformational changes that peel 50 bp of DNA from the edge of nucleosome core particles,and create a "bulge" in the DNA:core nucleosomes association?

Accepted Answer

A) histone deacetylase complexes 
B) DNA-bound transcriptional activators 
C) chromatin-remodelling complexes 
D) general transcriptional factors 
A) histone deacetylase complexes 
B) DNA-bound transcriptional activators 
C) chromatin-remodelling complexes 
D) general transcriptional factors

Question 18

Which of the following terms defines nucleotide sequences that identify the location of transcription start sites and regulate the level of transcription?

Accepted Answer

A) sigma factors 
B) Pribnow boxes 
C) TATA boxes 
D) promoters 
A) sigma factors 
B) Pribnow boxes 
C) TATA boxes 
D) promoters

Question 19

Which of the following is NOT a component of the lac operon?

Accepted Answer

A) the structural genes for lacZ,lacY,and lacA 
B) the operator and promoter sequences 
C) a lacI gene 
D) a separate operator for the lacI gene 
A) the structural genes for lacZ,lacY,and lacA 
B) the operator and promoter sequences 
C) a lacI gene 
D) a separate operator for the lacI gene

Question 20

The non-template strand has the same nucleotide sequence and direction as that of the RNA transcript.Which of the following parts of the mRNA encodes the N-terminus of the protein?

Accepted Answer

A) non-reducing end 
B) reducing end 
C) 3'-end 
D) 5'-end 
A) non-reducing end 
B) reducing end 
C) 3'-end 
D) 5'-end

Exam 29: Transcription and the Regulation of Gene Expression

Which of the following is needed for an inducible operon to be transcribed?

Which of the following occurs when the heptapeptide repeat in the carboxy terminal domain in the RPB1 subunit of RNA polymerase II is phosphorylated?

Which of the following recurring structural features is found in DNA-binding proteins?

The stand of dsDNA that is read by RNA polymerase is termed the template strand with RNA polymerase moving 3'?5' along it.Which of the following does the polymerase form,and in which direction does it grow?

Which of the following is NOT classified as a spliceosome?

Which of the following is NOT a stage in transcription?

Heat shock element (HSE)is a response element found in the promoter region of genes.How is transcription of the HSE activated?

The initiation site binds a purine nucleotide base pairing with the +1 base exposed within the open promoter complex.Which of the following is where the second base is added?

There are 2 consensus sequence elements within the promoter sequence.Which of the following are they?

Which of the following is thought to be generated by transcription chain elongation?

Which of the following is a characteristic of protein isoforms?

Which of the following is a characteristic of the Zn-finger motif?

Which of the following is NOT a control mechanism that regulates transcription in prokaryotes?

Which of the following is where are nucleotides said to lie relative to the transcription site when they are in the "minus" direction?

Which of the following eukaryotic transcription factors exhibits sequence specificity for the TATA box?

Which of the following DNA-binding motifs common to many prokaryotic regulatory proteins is the lac repressor an example of?

Which of the following terms defines the huge multi-subunit entities that mediate ATP-dependent conformational changes that peel 50 bp of DNA from the edge of nucleosome core particles,and create a "bulge" in the DNA:core nucleosomes association?

Which of the following terms defines nucleotide sequences that identify the location of transcription start sites and regulate the level of transcription?

Which of the following is NOT a component of the lac operon?

The non-template strand has the same nucleotide sequence and direction as that of the RNA transcript.Which of the following parts of the mRNA encodes the N-terminus of the protein?

The Facts of Life: Chemistry Is the Logic of Biological Phenomena

Water: the Medium of Life

Thermodynamics of Biological Systems

Amino Acids

Proteins: Their Primary Structure and Biological Functions

Proteins: Secondary, tertiary, and Quaternary Structure

Carbohydrates and the Glycoconjugates of Cell Surfaces

Lipids

Lipid Biosynthesis

Membranes and Membrane Transport

The Reception and Transmission of Extracellular Information

Enzymes Kinetics and Specificity

Mechanisms of Enzyme Action

Enzyme Regulation

Molecular Motors

Nutrition and the Organization of Metabolism

Glycolysis

The Tricarboxylic Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Gluconeogenesis, glycogen Metabolism, and the Pentose Phosphate Pathway

Fatty Acid Catabolism

Nitrogen Acquisition and Amino Acid Metabolism

Nucleotides and Nucleic Acids

Structure of Nucleic Acids

Synthesis and Degradation of Nucleotides

Recombinant Dna: Cloning and Creation of Chimeric Genes

DNA Metabolism: Replication, recombination, and Repair

Protein Synthesis

Post-Transcriptional Regulation of Gene Expression

Completing the Protein Life Cycle: Folding, processing, and Degradation

Metabolic Integration and Organ Specialization

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