Question 1

Acidophiles would be expected to grow best at a pH of

Accepted Answer

A)  3 
B)  6 
C)  9 
D)  12 
A)  3 
B)  6 
C)  9 
D)  12

Question 2

______ techniques must be strictly adhered to to prevent the accidental contamination of stock cultures

Accepted Answer

A)  Germination 
B)  Selective 
C)  Differential 
D)  Aseptic 
A)  Germination 
B)  Selective 
C)  Differential 
D)  Aseptic

Question 3

Which of the following is not one of the phases of bacterial growth?

Accepted Answer

A)  lag phase 
B)  stationary phase 
C)  doubling phase 
D)  log phase 
A)  lag phase 
B)  stationary phase 
C)  doubling phase 
D)  log phase

Question 4

A bacterial culture can be kept in the log phase of growth indefinitely with the help of a

Accepted Answer

A)  incubator 
B)  chemostat 
C)  spectrophotometer 
D)  colony counter 
A)  incubator 
B)  chemostat 
C)  spectrophotometer 
D)  colony counter

Question 5

Endospores are

Accepted Answer

A)  metabolically active 
B)  reproductive structures 
C)  found mostly in Gram negative organisms 
D)  protective structures 
A)  metabolically active 
B)  reproductive structures 
C)  found mostly in Gram negative organisms 
D)  protective structures

Question 6

Bacterial growth in colonies on agar plates

Accepted Answer

A)  have all phases of growth occurring simultaneously somewhere in the colony 
B)  most growth occurs at the center of the colony 
C)  involves budding of small new colonies from the surface of an existing colony 
D)  have lag phase cells in the center of the colony, surrounded by a ring of cells in exponential phase 
A)  have all phases of growth occurring simultaneously somewhere in the colony 
B)  most growth occurs at the center of the colony 
C)  involves budding of small new colonies from the surface of an existing colony 
D)  have lag phase cells in the center of the colony, surrounded by a ring of cells in exponential phase

Question 7

Which of the following counting techniques doesn't differentiate between live and dead bacterial cells in a culture?

Accepted Answer

A)  serial dilution 
B)  spread plate 
C)  pour plate 
D)  direct microscopic count 
A)  serial dilution 
B)  spread plate 
C)  pour plate 
D)  direct microscopic count

Question 8

An obligate psychrophile would produce a turbid culture at ________ degrees Celsius.

Accepted Answer

A)  15 
B)  30 
C)  45 
D)  60 
A)  15 
B)  30 
C)  45 
D)  60

Question 9

A decrease in the amount of nitrogen to a culture of Clostridium botulinum would induce

Accepted Answer

A)  activation 
B)  germination 
C)  sporulation 
D)  outgrowth 
A)  activation 
B)  germination 
C)  sporulation 
D)  outgrowth

Question 10

A growth medium consisting of only water,glucose and beef extract would be considered

Accepted Answer

A)  a defined synthetic medium 
B)  a complex medium 
C)  a chemically defined medium 
D)  two of the above 
A)  a defined synthetic medium 
B)  a complex medium 
C)  a chemically defined medium 
D)  two of the above

Question 11

A candle jar would be used for growing

Accepted Answer

A)  Gram negative organisms 
B)  fastidious organisms 
C)  thermophiles 
D)  anaerobes 
A)  Gram negative organisms 
B)  fastidious organisms 
C)  thermophiles 
D)  anaerobes

Question 12

Chocolate agar

Accepted Answer

A)  contains blood 
B)  is used exclusively for stock cultures 
C)  is nutrient poor 
D)  is a selective agar plate 
A)  contains blood 
B)  is used exclusively for stock cultures 
C)  is nutrient poor 
D)  is a selective agar plate

Question 13

Sporulation occurs in

Accepted Answer

A)  Clostridium 
B)  Staphylocoocus 
C)  Klebsiella 
D)  Citrobacter 
A)  Clostridium 
B)  Staphylocoocus 
C)  Klebsiella 
D)  Citrobacter

Question 14

The toxic effects of the byproducts of oxygen metabolism are mitigated by which of the following?

Accepted Answer

A)  catalase 
B)  superoxide dismutase 
C)  lactase 
D)  two of the above 
A)  catalase 
B)  superoxide dismutase 
C)  lactase 
D)  two of the above

Question 15

Most human pathogens are

Accepted Answer

A)  psychrophiles 
B)  mesophiles 
C)  thermophiles 
D)  acidophiles 
A)  psychrophiles 
B)  mesophiles 
C)  thermophiles 
D)  acidophiles

Exam 6: Growth and Culturing of Bacteria

Acidophiles would be expected to grow best at a pH of

______ techniques must be strictly adhered to to prevent the accidental contamination of stock cultures

Which of the following is not one of the phases of bacterial growth?

A bacterial culture can be kept in the log phase of growth indefinitely with the help of a

Endospores are

Bacterial growth in colonies on agar plates

Which of the following counting techniques doesn't differentiate between live and dead bacterial cells in a culture?

An obligate psychrophile would produce a turbid culture at ________ degrees Celsius.

A decrease in the amount of nitrogen to a culture of Clostridium botulinum would induce

A growth medium consisting of only water,glucose and beef extract would be considered

A candle jar would be used for growing

Chocolate agar

Sporulation occurs in

The toxic effects of the byproducts of oxygen metabolism are mitigated by which of the following?

Most human pathogens are

Scope and History of Microbiology

Fundamentals of Chemistry

Microscopy and Staining

Characteristics of Prokaryotic and Eukaryotic Cells

Essential Concepts of Metabolism

Microbial Genetics

Gene Transfer and Genetic Engineering

An Introduction to Taxonomy: the Bacteria

Viruses

Eukaryotic Microorganisms and Parasites

Sterilization and Disinfection

Antimicrobial Therapy

Host-Microbe Relationships and Disease Processes

Epidemiology and Nosocomial Infections

Innate Host Defenses

Immunology I: Basic Principles of Adaptive Immunity and Immunization

Immunolgy Ii: Immunological Disorders and Tests

Diseases of the Skin and Eyes; Wounds and Bites

Urogenital and Sexually Transmitted Diseases

Diseases of the Respiratory System

Oral and Gastrointestinal Diseases

Cardiovascular, lymphatic and Systemic Disease

Diseases of the Nervous System

Environmental Microbiology

Applied Microbiology

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