Question 1

Moist heat kills microorganisms by denaturing their

Accepted Answer

A)  lipids 
B)  proteins 
C)  carbohydrates 
D)  nucleic acids 
A)  lipids 
B)  proteins 
C)  carbohydrates 
D)  nucleic acids B

Question 2

Which is a good method to sterilize food?

Accepted Answer

A)  freezing 
B)  lyophilization 
C)  drying 
D)  two of the above 
A)  freezing 
B)  lyophilization 
C)  drying 
D)  two of the above C

Question 3

Dry heat does most of its damage against microorganisms by

Accepted Answer

A)  disrupting membrane lipids. 
B)  disrupting hydrogen bonds. 
C)  oxidizing molecules. 
D)  denaturing proteins. 
A)  disrupting membrane lipids. 
B)  disrupting hydrogen bonds. 
C)  oxidizing molecules. 
D)  denaturing proteins. C

Question 4

Which is not used to measure the effectiveness of an antimicrobial agent?

Accepted Answer

A)  filter paper method 
B)  phenol coefficient method 
C)  use-dilution method 
D)  plate count method 
A)  filter paper method 
B)  phenol coefficient method 
C)  use-dilution method 
D)  plate count method

Question 5

Which is not a practical means of killing microorganisms?

Accepted Answer

A)  pasteurization 
B)  freeze-drying 
C)  ultrasonic waves 
D)  ultraviolet light 
A)  pasteurization 
B)  freeze-drying 
C)  ultrasonic waves 
D)  ultraviolet light

Question 6

Agents that adversely affect nucleic acids and energy production include all of the following except

Accepted Answer

A)  detergents 
B)  alcohols 
C)  crystal violet 
D)  lactic acid 
A)  detergents 
B)  alcohols 
C)  crystal violet 
D)  lactic acid

Question 7

An agent that inhibits the growth of bacteria is called a (an)

Accepted Answer

A)  antiseptic 
B)  bactericide 
C)  bacteriostatic 
D)  sanitizer 
A)  antiseptic 
B)  bactericide 
C)  bacteriostatic 
D)  sanitizer

Question 8

Membrane filters are used for sterilization because

Accepted Answer

A)  filters with smallest pore size do not clog easily. 
B)  many materials are destroyed by heat sterilization 
C)  they are used to sterilize only very small amounts of liquid 
D)  they are impractical for the filtration of beer, sera, media, vitamins and drugs. 
A)  filters with smallest pore size do not clog easily. 
B)  many materials are destroyed by heat sterilization 
C)  they are used to sterilize only very small amounts of liquid 
D)  they are impractical for the filtration of beer, sera, media, vitamins and drugs.

Question 9

Denaturation of proteins by antimicrobial agents

Accepted Answer

A)  prevents viruses from infecting host cells 
B)  disrupts hydrogen and disulfide bonds 
C)  aids in preserving the shape and function of proteins 
D)  two of the above 
A)  prevents viruses from infecting host cells 
B)  disrupts hydrogen and disulfide bonds 
C)  aids in preserving the shape and function of proteins 
D)  two of the above

Question 10

Which of the following do not affect viruses?

Accepted Answer

A)  alkylating agents 
B)  detergents 
C)  alcohols 
D)  halogens 
A)  alkylating agents 
B)  detergents 
C)  alcohols 
D)  halogens

Question 11

What is the most susceptible phase of growth for most microorganisms by an antimicrobial agent?

Accepted Answer

A)  lag phase 
B)  log phase 
C)  stationary phase 
D)  death phase 
A)  lag phase 
B)  log phase 
C)  stationary phase 
D)  death phase

Question 12

Which is not used when trying to ensure that autoclaving has achieved sterility?

Accepted Answer

A)  internal devices to maintain proper temperature and pressure 
B)  tapes impregnated with the word "sterile" when proper temperature has been reached 
C)  pasteurization by heating to 62.9 °C for 30 minutes 
D)  autoclaving heat-resistant endospores such as Bacillus stearothermophilus 
A)  internal devices to maintain proper temperature and pressure 
B)  tapes impregnated with the word "sterile" when proper temperature has been reached 
C)  pasteurization by heating to 62.9 °C for 30 minutes 
D)  autoclaving heat-resistant endospores such as Bacillus stearothermophilus

Question 13

What makes a disinfectant desirable? Describe one method for determining the effectiveness of a chemical agent.

Accepted Answer

A disinfectant is desirable if it is: fa

Question 14

Chemical agents that denature proteins include all of the following except

Accepted Answer

A)  acids 
B)  alkalis 
C)  detergents 
D)  halogens 
A)  acids 
B)  alkalis 
C)  detergents 
D)  halogens

Question 15

Which of the following statements about detergents is true?

Accepted Answer

A)  detergents act as chemical mutagens of DNA or RNA 
B)  germicidal soaps are just as effective disinfectants as surgical scrubs 
C)  cationic detergents are effective in killing endospores 
D)  anionic detergents used for laundering clothes are less effective than cationic detergents 
A)  detergents act as chemical mutagens of DNA or RNA 
B)  germicidal soaps are just as effective disinfectants as surgical scrubs 
C)  cationic detergents are effective in killing endospores 
D)  anionic detergents used for laundering clothes are less effective than cationic detergents

Question 16

What is a chemical agent that can safely be used externally on living tissue to destroy microorganisms or to inhibit their growth?

Accepted Answer

A)  antiseptic 
B)  disinfectant 
C)  sanitizer 
D)  germicide 
A)  antiseptic 
B)  disinfectant 
C)  sanitizer 
D)  germicide

Question 17

An agent capable of killing microbes rapidly,perhaps killing certain microorganisms but only inhibiting the growth of others is called

Accepted Answer

A)  antiseptic 
B)  disinfectant 
C)  sanitizer 
D)  germicide 
A)  antiseptic 
B)  disinfectant 
C)  sanitizer 
D)  germicide

Question 18

Which of the following statements about evaluating the effectiveness of chemical agents is false?

Accepted Answer

A)  phenol coefficient can be used to evaluate the efficacy of phenol derivatives 
B)  materials present can complex with or inactivate a chemical agent during the phenol coefficient test 
C)  in the filter paper test, a clear area around the disk indicates a prevention of microorganism growth but not killing of microbes 
D)  the use-dilution test is considered to be more meaningful than the phenol coefficient 
A)  phenol coefficient can be used to evaluate the efficacy of phenol derivatives 
B)  materials present can complex with or inactivate a chemical agent during the phenol coefficient test 
C)  in the filter paper test, a clear area around the disk indicates a prevention of microorganism growth but not killing of microbes 
D)  the use-dilution test is considered to be more meaningful than the phenol coefficient

Question 19

All of the following are examples of general types of radiation except

Accepted Answer

A)  ultraviolet light 
B)  ionization 
C)  microwaves 
D)  ultrasonic waves 
A)  ultraviolet light 
B)  ionization 
C)  microwaves 
D)  ultrasonic waves

Question 20

Lyophilization is

Accepted Answer

A)  freeze-drying 
B)  refrigeration 
C)  freezing 
D)  heat-drying 
A)  freeze-drying 
B)  refrigeration 
C)  freezing 
D)  heat-drying

Exam 12: Sterilization and Disinfection

Moist heat kills microorganisms by denaturing their

Which is a good method to sterilize food?

Dry heat does most of its damage against microorganisms by

Which is not used to measure the effectiveness of an antimicrobial agent?

Which is not a practical means of killing microorganisms?

Agents that adversely affect nucleic acids and energy production include all of the following except

An agent that inhibits the growth of bacteria is called a (an)

Membrane filters are used for sterilization because

Denaturation of proteins by antimicrobial agents

Which of the following do not affect viruses?

What is the most susceptible phase of growth for most microorganisms by an antimicrobial agent?

Which is not used when trying to ensure that autoclaving has achieved sterility?

What makes a disinfectant desirable? Describe one method for determining the effectiveness of a chemical agent.

Chemical agents that denature proteins include all of the following except

Which of the following statements about detergents is true?

What is a chemical agent that can safely be used externally on living tissue to destroy microorganisms or to inhibit their growth?

An agent capable of killing microbes rapidly,perhaps killing certain microorganisms but only inhibiting the growth of others is called

Which of the following statements about evaluating the effectiveness of chemical agents is false?

All of the following are examples of general types of radiation except

Lyophilization is

Scope and History of Microbiology

Fundamentals of Chemistry

Microscopy and Staining

Characteristics of Prokaryotic and Eukaryotic Cells

Essential Concepts of Metabolism

Growth and Culturing of Bacteria

Microbial Genetics

Gene Transfer and Genetic Engineering

An Introduction to Taxonomy: the Bacteria

Viruses

Eukaryotic Microorganisms and Parasites

Antimicrobial Therapy

Host-Microbe Relationships and Disease Processes

Epidemiology and Nosocomial Infections

Innate Host Defenses

Immunology I: Basic Principles of Adaptive Immunity and Immunization

Immunolgy Ii: Immunological Disorders and Tests

Diseases of the Skin and Eyes; Wounds and Bites

Urogenital and Sexually Transmitted Diseases

Diseases of the Respiratory System

Oral and Gastrointestinal Diseases

Cardiovascular, lymphatic and Systemic Disease

Diseases of the Nervous System

Environmental Microbiology

Applied Microbiology

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