Question 1

A media on which Gram positive organisms turn green and Gram negative organisms turn blue would be

Accepted Answer

A)  selective 
B)  differential 
C)  complex 
D)  enriched 
A)  selective 
B)  differential 
C)  complex 
D)  enriched

Question 2

Nonculturable organisms

Accepted Answer

A)  are rare 
B)  can be identified based on their DNA 
C)  can only be seen on selective agar plates 
D)  have defined generation times 
A)  are rare 
B)  can be identified based on their DNA 
C)  can only be seen on selective agar plates 
D)  have defined generation times

Question 3

Turbidity in a bacterial culture can be measured using a/n

Accepted Answer

A)  incubator 
B)  colony county 
C)  spectrophotometer 
D)  chemostat 
A)  incubator 
B)  colony county 
C)  spectrophotometer 
D)  chemostat

Question 4

A living,growing culture whose purpose is to maintain a pure culture of an organism indefinitely is a

Accepted Answer

A)  colony 
B)  stock culture 
C)  sporulating culture 
D)  broth culture 
A)  colony 
B)  stock culture 
C)  sporulating culture 
D)  broth culture

Question 5

Which of the following types of organisms would be most likely to have evolved to live in the large intestine,where there is a complete lack of free oxygen?

Accepted Answer

A)  obligate aerobe 
B)  obligate anaerobe 
C)  facultative anaerobe 
D)  aerotolerant anaerobe 
A)  obligate aerobe 
B)  obligate anaerobe 
C)  facultative anaerobe 
D)  aerotolerant anaerobe

Question 6

Which of the following statements about bacteria growth is false?

Accepted Answer

A)  agar is used as a solidifying agent in some types of media 
B)  bacteria growing in a liquid culture will generate colonies 
C)  a turbid culture is indicative of bacterial growth 
D)  each bacterium plated will represent a colony-forming unit 
A)  agar is used as a solidifying agent in some types of media 
B)  bacteria growing in a liquid culture will generate colonies 
C)  a turbid culture is indicative of bacterial growth 
D)  each bacterium plated will represent a colony-forming unit

Question 7

When cells are placed in a hypertonic environment they will undergo ________ .

Accepted Answer

A)  lysis 
B)  no change in size 
C)  swelling of the cell which is contained by the cell wall 
D)  plasmolysis 
A)  lysis 
B)  no change in size 
C)  swelling of the cell which is contained by the cell wall 
D)  plasmolysis

Question 8

Serial dilutions are used to

Accepted Answer

A)  slow down the growth of microbes 
B)  speed up the growth of microbes 
C)  obtain bacterial cultures at several different concentrations 
D)  form bacterial colonies 
A)  slow down the growth of microbes 
B)  speed up the growth of microbes 
C)  obtain bacterial cultures at several different concentrations 
D)  form bacterial colonies

Question 9

Most bacteria reproduce by

Accepted Answer

A)  sexual reproduction 
B)  binary fission 
C)  budding 
D)  homologous recombination 
A)  sexual reproduction 
B)  binary fission 
C)  budding 
D)  homologous recombination

Question 10

Which of the following statements about media and agar is false?

Accepted Answer

A)  both chocolate agar and blood agar contain blood 
B)  stock cultures are never directly used for laboratory studies 
C)  a differential media will allow some bacteria to grow and stop the growth of others 
D)  a selective media will suppress the growth of some microbes 
A)  both chocolate agar and blood agar contain blood 
B)  stock cultures are never directly used for laboratory studies 
C)  a differential media will allow some bacteria to grow and stop the growth of others 
D)  a selective media will suppress the growth of some microbes

Question 11

Which of the following is not a stage in endospores returning to vegetative state?

Accepted Answer

A)  traumatic agent such as low pH or heat damages the coat 
B)  outgrowth occurs in a medium with adequate nutrients 
C)  divisions of the endospores early in their development 
D)  water and nutrients penetrate damaged coat 
A)  traumatic agent such as low pH or heat damages the coat 
B)  outgrowth occurs in a medium with adequate nutrients 
C)  divisions of the endospores early in their development 
D)  water and nutrients penetrate damaged coat

Question 12

In the stationary phase of bacterial growth

Accepted Answer

A)  The number of newly generated cells is greater than the number of dying cells 
B)  The number of newly generated cells is less than the number of dying cells 
C)  The number of newly generated cells is the same as the number of dying cells 
D)  none of the above 
A)  The number of newly generated cells is greater than the number of dying cells 
B)  The number of newly generated cells is less than the number of dying cells 
C)  The number of newly generated cells is the same as the number of dying cells 
D)  none of the above

Question 13

The streak plate method produces pure cultures by separating individual bacterial cells from one another by

Accepted Answer

A)  dragging them across a solid surface 
B)  dispersing them in liquid media 
C)  killing off those cells that are not able to survive in hot agar 
D)  embedding some cells in the agar so that they are exposed to lower concentrations of oxygen 
A)  dragging them across a solid surface 
B)  dispersing them in liquid media 
C)  killing off those cells that are not able to survive in hot agar 
D)  embedding some cells in the agar so that they are exposed to lower concentrations of oxygen

Question 14

A bacterial population grows most rapidly during ________ phase.

Accepted Answer

A)  lag 
B)  log 
C)  stationary 
D)  death 
A)  lag 
B)  log 
C)  stationary 
D)  death

Question 15

The pour plate method of isolating pure cultures

Accepted Answer

A)  relies on the fact that liquid agar will solidify as it cools 
B)  is useful for growing microaerophiles 
C)  results in some organisms growing embedded in the agar 
D)  all of the above 
A)  relies on the fact that liquid agar will solidify as it cools 
B)  is useful for growing microaerophiles 
C)  results in some organisms growing embedded in the agar 
D)  all of the above

Question 16

Imagine NASA sends you a specimen from a planet that they believe contains an extraterrestial bacteria species. You are the microbiologist in charge of determining how to grow the extraterrestial bacteria. Many factors may affect the growth of this extraterrestial bacteria. Choose two and describe how you would test their effects.

Accepted Answer

Physical factors that could affect the g

Question 17

Microbial growth

Accepted Answer

A)  refers to the increase in the size of a microbial cell 
B)  refers to the increase in the frequency of cell division 
C)  in a single generation's time leads to double the cell size 
D)  in a single generation's time leads to double the number of microbes. 
A)  refers to the increase in the size of a microbial cell 
B)  refers to the increase in the frequency of cell division 
C)  in a single generation's time leads to double the cell size 
D)  in a single generation's time leads to double the number of microbes.

Question 18

Exoenzymes

Accepted Answer

A)  produced by gram positive rods act in the medium around the organism 
B)  are released by the golgi apparatus into the cytoplasm where they act 
C)  produced by gram negative rods act in the periplamic space 
D)  two of the above 
A)  produced by gram positive rods act in the medium around the organism 
B)  are released by the golgi apparatus into the cytoplasm where they act 
C)  produced by gram negative rods act in the periplamic space 
D)  two of the above

Question 19

Capnophiles grow best under conditions of

Accepted Answer

A)  high carbon dioxide 
B)  low carbon dioxide 
C)  high osmotic pressure 
D)  low osmotic pressure 
A)  high carbon dioxide 
B)  low carbon dioxide 
C)  high osmotic pressure 
D)  low osmotic pressure

Question 20

Halophiles require an environment with a high concentration of ________ for optimal growth.

Accepted Answer

A)  sugar 
B)  alcohol 
C)  salt 
D)  phosphorous 
A)  sugar 
B)  alcohol 
C)  salt 
D)  phosphorous

Exam 6: Growth and Culturing of Bacteria

A media on which Gram positive organisms turn green and Gram negative organisms turn blue would be

Nonculturable organisms

Turbidity in a bacterial culture can be measured using a/n

A living,growing culture whose purpose is to maintain a pure culture of an organism indefinitely is a

Which of the following types of organisms would be most likely to have evolved to live in the large intestine,where there is a complete lack of free oxygen?

Which of the following statements about bacteria growth is false?

When cells are placed in a hypertonic environment they will undergo ________ .

Serial dilutions are used to

Most bacteria reproduce by

Which of the following statements about media and agar is false?

Which of the following is not a stage in endospores returning to vegetative state?

In the stationary phase of bacterial growth

The streak plate method produces pure cultures by separating individual bacterial cells from one another by

A bacterial population grows most rapidly during ________ phase.

The pour plate method of isolating pure cultures

Microbial growth

Exoenzymes

Capnophiles grow best under conditions of

Halophiles require an environment with a high concentration of ________ for optimal growth.

Scope and History of Microbiology

Fundamentals of Chemistry

Microscopy and Staining

Characteristics of Prokaryotic and Eukaryotic Cells

Essential Concepts of Metabolism

Microbial Genetics

Gene Transfer and Genetic Engineering

An Introduction to Taxonomy: the Bacteria

Viruses

Eukaryotic Microorganisms and Parasites

Sterilization and Disinfection

Antimicrobial Therapy

Host-Microbe Relationships and Disease Processes

Epidemiology and Nosocomial Infections

Innate Host Defenses

Immunology I: Basic Principles of Adaptive Immunity and Immunization

Immunolgy Ii: Immunological Disorders and Tests

Diseases of the Skin and Eyes; Wounds and Bites

Urogenital and Sexually Transmitted Diseases

Diseases of the Respiratory System

Oral and Gastrointestinal Diseases

Cardiovascular, lymphatic and Systemic Disease

Diseases of the Nervous System

Environmental Microbiology

Applied Microbiology

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