Exam 9: Transcriptional Regulation and Epigenetics

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Polycomb proteins are important in repressing gene transcription during development and differentiation. They act by _______ lysine residues in histones.

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Which statement about cis-acting elements is true?

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The diagram below illustrates how two _______-acting elements are positioned near the gene that they regulate. The diagram below illustrates how two _______-acting elements are positioned near the gene that they regulate.

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DNA methylation patterns in parental cells are established and maintained in progeny cells by which of the following mechanisms?

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Suppose a mutation renders a cell incapable of producing functional P-TEFb. What effect would the mutation have on this cell?

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The lac operator consists of approximately 20 base pairs of DNA located

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What is the role of the transcription factor c-Myc in human embryonic stem cells?

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How can epigenetic changes explain why identical twins develop different physical characteristics later in life?

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Which characteristic of chromatin would you expect to see increase when incubated with histone acetyltransferase enzymes?

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Some bacterial promoter regions are regulated by _______ that stimulate RNA polymerase binding and by _______ that block RNA polymerase binding.

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If you had genomic DNA and a purified transcription factor, how might you use that to determine the DNA sequence to which the transcription factor binds?

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The nucleus of a mature mouse skin cell is transplanted into an enucleated mouse embryo. The embryo develops into a blastocyst but then stalls at this stage. How can epigenetics explain this?

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A student made the following table to summarize what he had learned about bacterial transcriptional control mechanisms. A fellow classmate pointed out that the table is not accurate. Which statement describes the problem? Type of regulatory protein Operon transcriptional response to wild- type regulatory protein Operon transcriptional response to mutated regulatory protein (rendered nonfunctional) Positive control Activator On Off Negative control Repressor Off Off

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P-TEFb contains a _______ that phosphorylates NELF, DSIF, and serine 2 in the C-terminal domain of RNA polymerase, all of which lead to re-activation of RNA polymerase and transcriptional elongation.

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Eukaryotic gene repressor proteins are thought to act by binding to

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