Question 1

Methylene blue in EMB agar slightly inhibits

Accepted Answer

A)  gram-positives. 
B)  gram-negatives. 
C)  viral plaques. 
D)  both gram-positives and gram-negatives. 
A)  gram-positives. 
B)  gram-negatives. 
C)  viral plaques. 
D)  both gram-positives and gram-negatives.

Question 2

The tuberculin skin test looks to identify ________ specific to Mycobacterium tuberculosis.

Accepted Answer

A)  antibodies 
B)  antigens 
C)  delayed hypersensitive (DTH) TH1 cells 
D)  delayed hypersensitive (DTH) TC cells 
A)  antibodies 
B)  antigens 
C)  delayed hypersensitive (DTH) TH1 cells 
D)  delayed hypersensitive (DTH) TC cells

Question 3

Which bacterium shows a characteristic metallic green sheen when grown on eosin-methylene blue (EMB) agar?

Accepted Answer

A)  Escherichia coli 
B)  Salmonella enterica 
C)  Staphylococcus aureus 
D)  Streptococcus pyogenes 
A)  Escherichia coli 
B)  Salmonella enterica 
C)  Staphylococcus aureus 
D)  Streptococcus pyogenes

Question 4

A gram-negative, oxidase-positive diplococcus growing on chocolate agar likely belongs to the genus

Accepted Answer

A)  Neisseria. 
B)  Escherichia. 
C)  Mycobacterium. 
D)  Pseudomonas. 
A)  Neisseria. 
B)  Escherichia. 
C)  Mycobacterium. 
D)  Pseudomonas.

Question 5

The first antibiotic to be characterized was a

Accepted Answer

A)  cephalosporin. 
B)  quinolone. 
C)  β-lactam. 
D)  macrolide. 
A)  cephalosporin. 
B)  quinolone. 
C)  β-lactam. 
D)  macrolide.

Question 6

A mechanism for penicillin resistance in bacteria is

Accepted Answer

A)  acetylation of the antibiotic. 
B)  phosphorylation of the antibiotic. 
C)  confirmation change of the antibiotic. 
D)  splitting the β-lactam ring of the antibiotic. 
A)  acetylation of the antibiotic. 
B)  phosphorylation of the antibiotic. 
C)  confirmation change of the antibiotic. 
D)  splitting the β-lactam ring of the antibiotic.

Question 7

Salmonella is commonly associated with wound infections.

Accepted Answer

A) True 
 B)False

Question 8

Some antibiotics inhibit protein synthesis by disruption of translation through interactions with the

Accepted Answer

A)  ribosome. 
B)  protein. 
C)  DNA. 
D)  chromosome. 
A)  ribosome. 
B)  protein. 
C)  DNA. 
D)  chromosome.

Question 9

Literally hundreds of clinically useful EIAs have been developed.

Accepted Answer

A) True 
 B)False

Question 10

The oxygen-free gas commonly employed during anoxic incubation of a bacterial culture is a mixture of

Accepted Answer

A)  nitrogen and carbon dioxide. 
B)  nitrogen and carbon monoxide. 
C)  nitrogen and argon. 
D)  nitrogen and hydrogen. 
A)  nitrogen and carbon dioxide. 
B)  nitrogen and carbon monoxide. 
C)  nitrogen and argon. 
D)  nitrogen and hydrogen.

Question 11

MacConkey agar and eosin-methylene blue (EMB) agar are ________ media.

Accepted Answer

A)  selective but not differential 
B)  differential but not selective 
C)  both selective and differential 
D)  neither selective nor differential 
A)  selective but not differential 
B)  differential but not selective 
C)  both selective and differential 
D)  neither selective nor differential

Question 12

EMB agar preferentially selects for the growth of ________ bacteria.

Accepted Answer

A)  gram-positive 
B)  gram-negative 
C)  gram-variable 
D)  both gram-positive and gram-negative 
A)  gram-positive 
B)  gram-negative 
C)  gram-variable 
D)  both gram-positive and gram-negative

Question 13

Which of the following must be accomplished FIRST in order to conduct RT-PCR?

Accepted Answer

A)  Complementary DNA must be synthesized from double-stranded DNA. 
B)  Complementary DNA must be synthesized from RNA. 
C)  Complementary DNA must be synthesized from proteins. 
D)  Complementary DNA must be synthesized from single-stranded DNA. 
A)  Complementary DNA must be synthesized from double-stranded DNA. 
B)  Complementary DNA must be synthesized from RNA. 
C)  Complementary DNA must be synthesized from proteins. 
D)  Complementary DNA must be synthesized from single-stranded DNA.

Question 14

SYBR Green, commonly used in qPCR, binds nonspecifically to ________, indicating the presence of the PCR product.

Accepted Answer

A)  ssDNA 
B)  dsDNA 
C)  ssRNA 
D)  dsRNA 
A)  ssDNA 
B)  dsDNA 
C)  ssRNA 
D)  dsRNA

Question 15

Obligately anaerobic normal flora are found in ________, which is/are an anoxic habitat(s) of the body.

Accepted Answer

A)  the kidneys 
B)  the skin 
C)  portions of the oral cavity 
D)  the lungs 
A)  the kidneys 
B)  the skin 
C)  portions of the oral cavity 
D)  the lungs

Question 16

What is the drawback of live attenuated vaccines?

Accepted Answer

A)  They require more secondary reimmunizations than other vaccines. 
B)  They are not effective in adults. 
C)  They do NOT provide a long-lasting secondary immune response. 
D)  They can cause disease in some immunocompromised individuals. 
A)  They require more secondary reimmunizations than other vaccines. 
B)  They are not effective in adults. 
C)  They do NOT provide a long-lasting secondary immune response. 
D)  They can cause disease in some immunocompromised individuals.

Question 17

Some infectious agents, such as Neisseria gonorrhoeae, do NOT elicit a systemic immune response.

Accepted Answer

A) True 
 B)False

Question 18

The basis for blood typing is referred to as

Accepted Answer

A)  neutralization. 
B)  hemagglutination. 
C)  immunodiffusion. 
D)  precipitation. 
A)  neutralization. 
B)  hemagglutination. 
C)  immunodiffusion. 
D)  precipitation.

Question 19

Laboratories that work with extremely low risk pathogens are classified as

Accepted Answer

A)  BSL-1. 
B)  BSL-2. 
C)  BSL-3. 
D)  BSL-4. 
A)  BSL-1. 
B)  BSL-2. 
C)  BSL-3. 
D)  BSL-4.

Question 20

Which of the following can be used in creating a vaccine?

Accepted Answer

A)  attenuated bacteria 
B)  inactivated viruses 
C)  recombinant proteins 
D)  attenuated bacteria, inactivated viruses, or recombinant proteins 
A)  attenuated bacteria 
B)  inactivated viruses 
C)  recombinant proteins 
D)  attenuated bacteria, inactivated viruses, or recombinant proteins

Exam 28: Clinical Microbiology and Immunology

Methylene blue in EMB agar slightly inhibits

The tuberculin skin test looks to identify ________ specific to Mycobacterium tuberculosis.

Which bacterium shows a characteristic metallic green sheen when grown on eosin-methylene blue (EMB) agar?

A gram-negative, oxidase-positive diplococcus growing on chocolate agar likely belongs to the genus

The first antibiotic to be characterized was a

A mechanism for penicillin resistance in bacteria is

Salmonella is commonly associated with wound infections.

Some antibiotics inhibit protein synthesis by disruption of translation through interactions with the

Literally hundreds of clinically useful EIAs have been developed.

The oxygen-free gas commonly employed during anoxic incubation of a bacterial culture is a mixture of

MacConkey agar and eosin-methylene blue (EMB) agar are ________ media.

EMB agar preferentially selects for the growth of ________ bacteria.

Which of the following must be accomplished FIRST in order to conduct RT-PCR?

SYBR Green, commonly used in qPCR, binds nonspecifically to ________, indicating the presence of the PCR product.

Obligately anaerobic normal flora are found in ________, which is/are an anoxic habitat(s) of the body.

What is the drawback of live attenuated vaccines?

Some infectious agents, such as Neisseria gonorrhoeae, do NOT elicit a systemic immune response.

The basis for blood typing is referred to as

Laboratories that work with extremely low risk pathogens are classified as

Which of the following can be used in creating a vaccine?

The Microbial World

Microbial Cell Structure and Function

Microbial Metabolism

Molecular Information Flow and Protein Processing

Microbial Growth and Its Control

Microbial Regulatory Systems

Molecular Biology of Microbial Growth

Viruses and Their Replication

Microbial Systems Biology

Viral Genomics, Diversity, and Ecology

Genetics of Bacteria and Archaea

Biotechnology and Synthetic Biology

Microbial Evolution and Systematics

Metabolic Diversity of Microorganisms

Functional Diversity of Microorganisms

Diversity of Bacteria

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Diversity of Microbial Eukarya

Taking the Measure of Microbial Systems

Microbial Ecosystems

Nutrient Cycles

Microbiology of the Built Environment

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Microbial Symbioses with Humans

Microbial Infection and Pathogenesis

Innate Immunity: Broadly Specific Host Defenses

Adaptive Immunity: Highly Specific Host Defenses

Epidemiology

Person-to-Person Bacterial and Viral Diseases

Vectorborne and Soilborne Bacterial and Viral Diseases

Waterborne and Foodborne Bacterial and Viral Diseases

Eukaryotic Pathogens: Fungi, Protozoans, and Helminths

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