Question 1

Colored photos of electron micrographs:

Accepted Answer

A) reflect the color of the specimen before it was frozen 
B) are false color added on during image preparation 
C) reflect the color of the specimen after it was frozen 
D) are always in pastel shades 
A) reflect the color of the specimen before it was frozen 
B) are false color added on during image preparation 
C) reflect the color of the specimen after it was frozen 
D) are always in pastel shades

Question 2

A microscope in which light rays pass directly through a specimen is a ________ microscope.

Accepted Answer

A) bright field 
B) dark field 
C) phase-contrast 
D) Nomarski 
A) bright field 
B) dark field 
C) phase-contrast 
D) Nomarski

Question 3

The lens closest to the slide during a microscopic examination is the _____.

Accepted Answer

A) ocular 
B) objective 
C) condenser 
D) compound 
A) ocular 
B) objective 
C) condenser 
D) compound

Question 4

Why do basic dyes attach to most bacterial surfaces

Accepted Answer

A) Most bacterial surfaces are negatively charged. 
B) Bacterial cells take up safranin. 
C) Most bacterial surfaces resist taking up the stain. 
D) Most bacterial surfaces do not have a charge. 
A) Most bacterial surfaces are negatively charged. 
B) Bacterial cells take up safranin. 
C) Most bacterial surfaces resist taking up the stain. 
D) Most bacterial surfaces do not have a charge.

Question 5

Which of the following stains has been used on this microorganism

Accepted Answer

A) Flagellar stain 
B) Capsule stain 
C) Endospore stain 
D) No stain 
A) Flagellar stain 
B) Capsule stain 
C) Endospore stain 
D) No stain

Question 6

The formula for the resolving power (resolution distance)of a lens is λ/2NA (wavelength /2 x numerical aperture).What does this say about resolving power

Accepted Answer

A) The smaller the wavelength,the greater the resolving power of the lens. 
B) Resolving power is not related to the lens' numerical aperture. 
C) We cannot precisely calculate a lens resolving power. 
D) A larger resolving power is indicative of a better lens. 
A) The smaller the wavelength,the greater the resolving power of the lens. 
B) Resolving power is not related to the lens' numerical aperture. 
C) We cannot precisely calculate a lens resolving power. 
D) A larger resolving power is indicative of a better lens.

Question 7

Light of ________ wavelength typically will result in ________ resolution.

Accepted Answer

A) longer,better 
B) shorter,better 
C) any,poor 
D) shorter,worse 
A) longer,better 
B) shorter,better 
C) any,poor 
D) shorter,worse

Question 8

When light passes through an object,________ of the light has occurred.

Accepted Answer

A) reflection 
B) absorption 
C) transmission 
D) fluorescence 
A) reflection 
B) absorption 
C) transmission 
D) fluorescence

Question 9

Three dimensional views of cells and other small object could best be obtained using a:

Accepted Answer

A) phase contrast microscope 
B) dark-field microscope 
C) transmission electron microscope (TEM) 
D) scanning electron microscope (SEM) 
A) phase contrast microscope 
B) dark-field microscope 
C) transmission electron microscope (TEM) 
D) scanning electron microscope (SEM)

Question 10

What statement about microscopy and staining techniques is false

Accepted Answer

A) Many species look identical under the microscope. 
B) Staining and microscopic examination are usually all that is need to identify a microorganism. 
C) Microscopes are of little use unless the specimens are prepared properly. 
D) The degree of contrast is equally important as resolution and magnification. 
A) Many species look identical under the microscope. 
B) Staining and microscopic examination are usually all that is need to identify a microorganism. 
C) Microscopes are of little use unless the specimens are prepared properly. 
D) The degree of contrast is equally important as resolution and magnification.

Question 11

What is true about the index of refraction

Accepted Answer

A) If light rays are taken up by the object than it has a high index of refraction. 
B) Refraction measures the frequency of the light as it reflects from a material. 
C) Oil immersion lenses increase the problem of refraction. 
D) Light will bend as it passes through two substances with different indices of refraction. 
A) If light rays are taken up by the object than it has a high index of refraction. 
B) Refraction measures the frequency of the light as it reflects from a material. 
C) Oil immersion lenses increase the problem of refraction. 
D) Light will bend as it passes through two substances with different indices of refraction.

Question 12

A simple stain:

Accepted Answer

A) uses only a single dye. 
B) requires only one step to stain a slide. 
C) distinguishes between two different parts of an organism. 
D) is composed of an equal balance of acid and basic dyes. 
A) uses only a single dye. 
B) requires only one step to stain a slide. 
C) distinguishes between two different parts of an organism. 
D) is composed of an equal balance of acid and basic dyes.

Question 13

Most light microscopes contain a/an ________ that converges the light beam so that it passes through the specimen.

Accepted Answer

A) objective lens 
B) iris diaphragm 
C) mechanical stage 
D) condenser 
A) objective lens 
B) iris diaphragm 
C) mechanical stage 
D) condenser

Colored photos of electron micrographs:

A microscope in which light rays pass directly through a specimen is a ________ microscope.

The lens closest to the slide during a microscopic examination is the _____.

Why do basic dyes attach to most bacterial surfaces

Which of the following stains has been used on this microorganism

The formula for the resolving power (resolution distance)of a lens is λ/2NA (wavelength /2 x numerical aperture).What does this say about resolving power

Light of wavelength typically will result in resolution.

When light passes through an object,________ of the light has occurred.

Three dimensional views of cells and other small object could best be obtained using a:

What statement about microscopy and staining techniques is false

What is true about the index of refraction

A simple stain:

Most light microscopes contain a/an ________ that converges the light beam so that it passes through the specimen.

Scope and History of Microbiology

The Microbiome

Fundamentals of Chemistry

Characteristics of Prokaryotic and Eukaryotic Cells

Essential Concepts of Metabolism

Growth and Culturing of Bacteria

Microbial Genetics

Gene Transfer and Genetic Engineering

An Introduction to Taxonomy: The Bacteria

Viruses

Eukaryotic Microorganisms and Parasites

Sterilization and Disinfection

Antimicrobial Therapy

Host-Microbe Relationships and Disease Processes

Epidemiology and Nosocomial Infections

Innate Host Defenses

Basic Principles of Adaptive Immunity and Immunization

Immune Disorders

Diseases of the Skin and Eyes; Wounds and Bites

Urogenital and Sexually Transmitted Diseases

Diseases of the Respiratory System

Oral and Gastrointestinal Diseases

Cardiovascular, Lymphatic, and Systemic Diseases

Diseases of the Nervous System

Environmental Microbiology

Applied Microbiology

Filters

Exam 4: Microscopy and Staining

Colored photos of electron micrographs:

A microscope in which light rays pass directly through a specimen is a ________ microscope.

The lens closest to the slide during a microscopic examination is the _____.

Why do basic dyes attach to most bacterial surfaces

Which of the following stains has been used on this microorganism

The formula for the resolving power (resolution distance)of a lens is λ/2NA (wavelength /2 x numerical aperture).What does this say about resolving power

Light of ________ wavelength typically will result in ________ resolution.

When light passes through an object,________ of the light has occurred.

Three dimensional views of cells and other small object could best be obtained using a:

What statement about microscopy and staining techniques is false

What is true about the index of refraction

A simple stain:

Most light microscopes contain a/an ________ that converges the light beam so that it passes through the specimen.

Scope and History of Microbiology

The Microbiome

Fundamentals of Chemistry

Characteristics of Prokaryotic and Eukaryotic Cells

Essential Concepts of Metabolism

Growth and Culturing of Bacteria

Microbial Genetics

Gene Transfer and Genetic Engineering

An Introduction to Taxonomy: The Bacteria

Viruses

Eukaryotic Microorganisms and Parasites

Sterilization and Disinfection

Antimicrobial Therapy

Host-Microbe Relationships and Disease Processes

Epidemiology and Nosocomial Infections

Innate Host Defenses

Basic Principles of Adaptive Immunity and Immunization

Immune Disorders

Diseases of the Skin and Eyes; Wounds and Bites

Urogenital and Sexually Transmitted Diseases

Diseases of the Respiratory System

Oral and Gastrointestinal Diseases

Cardiovascular, Lymphatic, and Systemic Diseases

Diseases of the Nervous System

Environmental Microbiology

Applied Microbiology

Filters

Light of wavelength typically will result in resolution.