Question 1

Which of the following statements about Leeuwenhoek's microscopes is false

Accepted Answer

A) Leeuwenhoek kept his technique secret. 
B) They magnified objects 100 to 300 times. 
C) For each specimen a new microscope had to be made. 
D) They were able to reveal very fine details of bacteria. 
A) Leeuwenhoek kept his technique secret. 
B) They magnified objects 100 to 300 times. 
C) For each specimen a new microscope had to be made. 
D) They were able to reveal very fine details of bacteria.

Question 2

A parfocal microscope:

Accepted Answer

A) has more than one source of illumination 
B) has both coarse and fine focusing adjustment 
C) accentuates small differences in the refractive index of intracellular structures 
D) allows for specimens to remain in focus when changing between magnification 
A) has more than one source of illumination 
B) has both coarse and fine focusing adjustment 
C) accentuates small differences in the refractive index of intracellular structures 
D) allows for specimens to remain in focus when changing between magnification

Question 3

Electron microscopes:

Accepted Answer

A) that are scanning have better resolution than those that are transmission 
B) are much more expensive and take up more space than light microscopes 
C) can use the same preparations of specimens that have been prepared for viewing with a light microscope 
D) have a resolving power approximately 10 times better than the best light microscope 
A) that are scanning have better resolution than those that are transmission 
B) are much more expensive and take up more space than light microscopes 
C) can use the same preparations of specimens that have been prepared for viewing with a light microscope 
D) have a resolving power approximately 10 times better than the best light microscope

Question 4

A microscope that converts changes in the speed of light as it passes through an object into differences in brightness is a ________ microscope.

Accepted Answer

A) bright field 
B) dark field 
C) phase-contrast 
D) Nomarski 
A) bright field 
B) dark field 
C) phase-contrast 
D) Nomarski

Question 5

Which stain would be the best choice for detecting mycobacterium (the bacteria responsible for tuberculosis and leprosy)

Accepted Answer

A) Simple stain 
B) Endospore stain 
C) Acid-fast stain 
D) Gram stain 
A) Simple stain 
B) Endospore stain 
C) Acid-fast stain 
D) Gram stain

Question 6

A compound microscope has _____.

Accepted Answer

A) two eyepieces 
B) a total magnification of 5,000X 
C) only fine adjustment and no coarse adjustment 
D) more than one lens 
A) two eyepieces 
B) a total magnification of 5,000X 
C) only fine adjustment and no coarse adjustment 
D) more than one lens

Question 7

The best electron microscopes have a resolution of _____ nm.

Accepted Answer

A) 0.1 
B) 1 
C) 10 
D) 100 
A) 0.1 
B) 1 
C) 10 
D) 100

Question 8

The counterstain in the endospore stain is _____.

Accepted Answer

A) malachite green 
B) crystal violet 
C) safranin 
D) methylene blue 
A) malachite green 
B) crystal violet 
C) safranin 
D) methylene blue

Question 9

Diffraction occurs when light _____.

Accepted Answer

A) is reflected by an object 
B) passes through a small opening 
C) changes wavelengths 
D) is absorbed by a normally transparent object (like a glass slide) 
A) is reflected by an object 
B) passes through a small opening 
C) changes wavelengths 
D) is absorbed by a normally transparent object (like a glass slide)

Question 10

Which of the following statements about resolution is true

Accepted Answer

A) Resolution refers to the ability of a lens to distinguish adjacent objects. 
B) With regard to light,resolution means the same thing as wavelength. 
C) Resolution refers to a microscope's ability to magnify objects. 
D) Resolution is equal to the distance between two adjacent crests of a wave. 
A) Resolution refers to the ability of a lens to distinguish adjacent objects. 
B) With regard to light,resolution means the same thing as wavelength. 
C) Resolution refers to a microscope's ability to magnify objects. 
D) Resolution is equal to the distance between two adjacent crests of a wave.

Question 11

When given a microorganism to identify,which of the following would be useful

Accepted Answer

A) A gram stain 
B) A transmission electron micrograph 
C) Biochemical and genetic characteristics 
D) All of these 
A) A gram stain 
B) A transmission electron micrograph 
C) Biochemical and genetic characteristics 
D) All of these

Question 12

Which of the following is not a differential stain

Accepted Answer

A) Gram stain 
B) Schaeffer-Fulton 
C) Acid-fast stain 
D) Flagellar stain 
A) Gram stain 
B) Schaeffer-Fulton 
C) Acid-fast stain 
D) Flagellar stain

Question 13

Electron microscopes have a much better resolving power when compared to light microscopes because electrons _____.

Accepted Answer

A) are invisible to the eye 
B) have longer wavelengths than visible light rays 
C) have shorter wavelengths than visible light rays 
D) are negatively charged 
A) are invisible to the eye 
B) have longer wavelengths than visible light rays 
C) have shorter wavelengths than visible light rays 
D) are negatively charged

Question 14

Atomic force microscope:

Accepted Answer

A) allows 3 dimensional imaging and measurement of structures as small as nucleotides in DNA 
B) is not yet capable of measuring small forces 
C) involves ultraviolet light exciting molecules so that they release light of a longer wavelength 
D) has a special condenser and objective lens 
A) allows 3 dimensional imaging and measurement of structures as small as nucleotides in DNA 
B) is not yet capable of measuring small forces 
C) involves ultraviolet light exciting molecules so that they release light of a longer wavelength 
D) has a special condenser and objective lens

Question 15

The term "basic dyes" refers to the fact that these dyes are _____.

Accepted Answer

A) easily prepared 
B) positively charged 
C) attracted to positively charged cell structures 
D) simple in their composition 
A) easily prepared 
B) positively charged 
C) attracted to positively charged cell structures 
D) simple in their composition

Question 16

Suitable stains for use in the Ziehl-Neelsen acid-fast stain are _____.

Accepted Answer

A) crystal violet and eosin 
B) carbolfuschin and methylene blue 
C) carbolfuschin and safranin 
D) safranin and methylene blue 
A) crystal violet and eosin 
B) carbolfuschin and methylene blue 
C) carbolfuschin and safranin 
D) safranin and methylene blue

Question 17

In order to make use of light for a microscopic examination of an object the object must ________ or ________ light.

Accepted Answer

A) absorb,luminesce 
B) transmit,absorb 
C) transmit,reflect 
D) reflect,absorb 
A) absorb,luminesce 
B) transmit,absorb 
C) transmit,reflect 
D) reflect,absorb

Question 18

Why is diffraction a problem for microscopy

Accepted Answer

A) The lens acts as a large aperture through which light must pass. 
B) The small size of higher-power lenses causes severe diffraction. 
C) The loss of light results in blurred images. 
D) Diffraction of light is useful when using an oil immersion lens to view objects. 
A) The lens acts as a large aperture through which light must pass. 
B) The small size of higher-power lenses causes severe diffraction. 
C) The loss of light results in blurred images. 
D) Diffraction of light is useful when using an oil immersion lens to view objects.

Question 19

Which of the following can be used to examine live specimens

Accepted Answer

A) TEM 
B) SEM 
C) scanning tunneling electron microscope 
D) atomic force microscope 
A) TEM 
B) SEM 
C) scanning tunneling electron microscope 
D) atomic force microscope

Question 20

Electron microscopes use ________ to focus the electron beam.

Accepted Answer

A) glass lenses 
B) electromagnets 
C) mechanical stages 
D) laser beams 
A) glass lenses 
B) electromagnets 
C) mechanical stages 
D) laser beams

Exam 4: Microscopy and Staining

Which of the following statements about Leeuwenhoek's microscopes is false

A parfocal microscope:

Electron microscopes:

A microscope that converts changes in the speed of light as it passes through an object into differences in brightness is a ________ microscope.

Which stain would be the best choice for detecting mycobacterium (the bacteria responsible for tuberculosis and leprosy)

A compound microscope has _____.

The best electron microscopes have a resolution of _____ nm.

The counterstain in the endospore stain is _____.

Diffraction occurs when light _____.

Which of the following statements about resolution is true

When given a microorganism to identify,which of the following would be useful

Which of the following is not a differential stain

Electron microscopes have a much better resolving power when compared to light microscopes because electrons _____.

Atomic force microscope:

The term "basic dyes" refers to the fact that these dyes are _____.

Suitable stains for use in the Ziehl-Neelsen acid-fast stain are _____.

In order to make use of light for a microscopic examination of an object the object must ________ or ________ light.

Why is diffraction a problem for microscopy

Which of the following can be used to examine live specimens

Electron microscopes use ________ to focus the electron beam.

Scope and History of Microbiology

The Microbiome

Fundamentals of Chemistry

Characteristics of Prokaryotic and Eukaryotic Cells

Essential Concepts of Metabolism

Growth and Culturing of Bacteria

Microbial Genetics

Gene Transfer and Genetic Engineering

An Introduction to Taxonomy: The Bacteria

Viruses

Eukaryotic Microorganisms and Parasites

Sterilization and Disinfection

Antimicrobial Therapy

Host-Microbe Relationships and Disease Processes

Epidemiology and Nosocomial Infections

Innate Host Defenses

Basic Principles of Adaptive Immunity and Immunization

Immune Disorders

Diseases of the Skin and Eyes; Wounds and Bites

Urogenital and Sexually Transmitted Diseases

Diseases of the Respiratory System

Oral and Gastrointestinal Diseases

Cardiovascular, Lymphatic, and Systemic Diseases

Diseases of the Nervous System

Environmental Microbiology

Applied Microbiology

Filters

In order to make use of light for a microscopic examination of an object the object must or light.