Question 1

A virus which incorporates its DNA into the DNA of its host organism is a

Accepted Answer

A)  virulent phage 
B)  prophage 
C)  lytic phage 
D)  none of the above 
A)  virulent phage 
B)  prophage 
C)  lytic phage 
D)  none of the above

Question 2

F' plasmids

Accepted Answer

A)  are responsible for high frequency recombination 
B)  carry some chromosomal genes 
C)  are those plasmids that have never been incorporated into a bacterial chromosome 
D)  none of the above 
A)  are responsible for high frequency recombination 
B)  carry some chromosomal genes 
C)  are those plasmids that have never been incorporated into a bacterial chromosome 
D)  none of the above

Question 3

Bacteriocins

Accepted Answer

A)  are resistance molecules found on plasmids 
B)  are inhibited by UV exposure 
C)  are growth-inhibiting proteins that inhibit strains closely related to the producer 
D)  only exist in eukaryotic organisms 
A)  are resistance molecules found on plasmids 
B)  are inhibited by UV exposure 
C)  are growth-inhibiting proteins that inhibit strains closely related to the producer 
D)  only exist in eukaryotic organisms

Question 4

The transfer of DNA between bacteria cells by a virus is

Accepted Answer

A)  transformation 
B)  transduction 
C)  transferration 
D)  conjugation 
A)  transformation 
B)  transduction 
C)  transferration 
D)  conjugation

Question 5

Gene amplification

Accepted Answer

A)  allows transposition of genes from one location on a chromosome to another 
B)  occurs when plasmids are induced to reproduce within cells at a rapid rate 
C)  allows material from one strain to recombine with that from another strain 
D)  complicate our ability to increase antibiotic production 
A)  allows transposition of genes from one location on a chromosome to another 
B)  occurs when plasmids are induced to reproduce within cells at a rapid rate 
C)  allows material from one strain to recombine with that from another strain 
D)  complicate our ability to increase antibiotic production

Question 6

Hybridomas are used to produce large quantities of

Accepted Answer

A)  restriction enzymes 
B)  monoclonal antibodies 
C)  antibiotics 
D)  natural insecticides 
A)  restriction enzymes 
B)  monoclonal antibodies 
C)  antibiotics 
D)  natural insecticides

Question 7

During the process of lysogeny

Accepted Answer

A)  a phage integrates into the chromosome of the bacterium 
B)  a bacterium takes up DNA from the media 
C)  new phage particles are assembled in the host bacterium 
D)  a bacterium is split open to release new phage particles 
A)  a phage integrates into the chromosome of the bacterium 
B)  a bacterium takes up DNA from the media 
C)  new phage particles are assembled in the host bacterium 
D)  a bacterium is split open to release new phage particles

Question 8

Hybridomas

Accepted Answer

A)  are responsible for tumor formation in plants 
B)  are resistant to monoclonal antibodies 
C)  divide and grow for only a short period of time 
D)  are the fusion of an antibody producing plasma cell and a cancer cell 
A)  are responsible for tumor formation in plants 
B)  are resistant to monoclonal antibodies 
C)  divide and grow for only a short period of time 
D)  are the fusion of an antibody producing plasma cell and a cancer cell

Question 9

In Griffith's transformation experiments,which of the following did not take place?

Accepted Answer

A)  live, smooth pneumococci killed mice 
B)  live rough bacteria were able to retrieve the DNA from dead, encapsulated bacteria and become virulent 
C)  DNA was transferred from smooth to rough bacteria using a bacteriophage 
D)  dead, smooth pneumococci had no effect on mice 
A)  live, smooth pneumococci killed mice 
B)  live rough bacteria were able to retrieve the DNA from dead, encapsulated bacteria and become virulent 
C)  DNA was transferred from smooth to rough bacteria using a bacteriophage 
D)  dead, smooth pneumococci had no effect on mice

Question 10

In a culture containing both F⁻ and F⁺ cells,which of the following will occur if we wait long enough?

Accepted Answer

A)  No conjugation will occur 
B)  The cells will all become genetically identical 
C)  All F⁻ will become F⁺ cells 
D)  All F⁺ cells will become F⁻ cells 
A)  No conjugation will occur 
B)  The cells will all become genetically identical 
C)  All F⁻ will become F⁺ cells 
D)  All F⁺ cells will become F⁻ cells

Question 11

Which of the following processes increases variation seen in bacteria?

Accepted Answer

A)  transformation 
B)  conjugation 
C)  transduction 
D)  all of the above 
A)  transformation 
B)  conjugation 
C)  transduction 
D)  all of the above

Question 12

In Griffith's experiments,the "transforming substance" was

Accepted Answer

A)  RNA 
B)  Protein 
C)  Polysaccharides 
D)  DNA 
A)  RNA 
B)  Protein 
C)  Polysaccharides 
D)  DNA

Question 13

Without restriction endonucleases,it would be impossible to

Accepted Answer

A)  insert plasmids into bacteria 
B)  bring about mutations in bacteria 
C)  replicate DNA in a recombinant cell 
D)  open the DNA of plasmids 
A)  insert plasmids into bacteria 
B)  bring about mutations in bacteria 
C)  replicate DNA in a recombinant cell 
D)  open the DNA of plasmids

Question 14

The Ti plasmid is known primarily for its ability to

Accepted Answer

A)  direct synthesis of conjugation pili 
B)  provide resistance to certain antibiotics 
C)  induce the formation of tumors in plants 
D)  two of the above 
A)  direct synthesis of conjugation pili 
B)  provide resistance to certain antibiotics 
C)  induce the formation of tumors in plants 
D)  two of the above

Question 15

Which of the following happens earliest in a typical bacteriophage lytic cycle?

Accepted Answer

A)  phage inserts its DNA into the host cell 
B)  phage DNA is inserted into the bacterial chromosome 
C)  bacterial cell lyses 
D)  viral DNA and proteins are produced by the host cell 
A)  phage inserts its DNA into the host cell 
B)  phage DNA is inserted into the bacterial chromosome 
C)  bacterial cell lyses 
D)  viral DNA and proteins are produced by the host cell

Question 16

Removing the cell walls from two different strains of organisms and then allowing the membrane bound cells to combine with one another best describes

Accepted Answer

A)  genetic fusion 
B)  protoplast fusion 
C)  cell fusion 
D)  DNA fusion 
A)  genetic fusion 
B)  protoplast fusion 
C)  cell fusion 
D)  DNA fusion

Question 17

Plasmids do which of the following

Accepted Answer

A)  direct synthesis of conjugation pili 
B)  provide resistance to certain antibiotics 
C)  induce the formation of tumors in plants 
D)  all of the above are functions of plasmids 
A)  direct synthesis of conjugation pili 
B)  provide resistance to certain antibiotics 
C)  induce the formation of tumors in plants 
D)  all of the above are functions of plasmids

Question 18

Soil bacteria are incredibly resistant to multiple antibiotics,presumably as a response to competition from bacteria that produce antibiotics. What are three ways that these antibiotic resistant genes could move from soil bacteria species to other species of bacteria? Briefly describe each gene transfer process. Name one or more factors that would affect the likelihood of this transfer occurring.

Accepted Answer

The four ways that genes can be passed f

Question 19

A lytic viral cycle is usually the result of infection by a

Accepted Answer

A)  prophage 
B)  virulent phage 
C)  temperate phage 
D)  antiphage 
A)  prophage 
B)  virulent phage 
C)  temperate phage 
D)  antiphage

Question 20

Recombinant bacteria have been used to produce

Accepted Answer

A)  human growth hormone 
B)  vaccines 
C)  insulin 
D)  all of the above 
A)  human growth hormone 
B)  vaccines 
C)  insulin 
D)  all of the above

Exam 8: Gene Transfer and Genetic Engineering

A virus which incorporates its DNA into the DNA of its host organism is a

F' plasmids

Bacteriocins

The transfer of DNA between bacteria cells by a virus is

Gene amplification

Hybridomas are used to produce large quantities of

During the process of lysogeny

Hybridomas

In Griffith's transformation experiments,which of the following did not take place?

In a culture containing both F⁻ and F⁺ cells,which of the following will occur if we wait long enough?

Which of the following processes increases variation seen in bacteria?

In Griffith's experiments,the "transforming substance" was

Without restriction endonucleases,it would be impossible to

The Ti plasmid is known primarily for its ability to

Which of the following happens earliest in a typical bacteriophage lytic cycle?

Removing the cell walls from two different strains of organisms and then allowing the membrane bound cells to combine with one another best describes

Plasmids do which of the following

A lytic viral cycle is usually the result of infection by a

Recombinant bacteria have been used to produce

Scope and History of Microbiology

Fundamentals of Chemistry

Microscopy and Staining

Characteristics of Prokaryotic and Eukaryotic Cells

Essential Concepts of Metabolism

Growth and Culturing of Bacteria

Microbial Genetics

An Introduction to Taxonomy: the Bacteria

Viruses

Eukaryotic Microorganisms and Parasites

Sterilization and Disinfection

Antimicrobial Therapy

Host-Microbe Relationships and Disease Processes

Epidemiology and Nosocomial Infections

Innate Host Defenses

Immunology I: Basic Principles of Adaptive Immunity and Immunization

Immunolgy Ii: Immunological Disorders and Tests

Diseases of the Skin and Eyes; Wounds and Bites

Urogenital and Sexually Transmitted Diseases

Diseases of the Respiratory System

Oral and Gastrointestinal Diseases

Cardiovascular, lymphatic and Systemic Disease

Diseases of the Nervous System

Environmental Microbiology

Applied Microbiology

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