Question 1

Most commonly, proteins interact with DNA at

Accepted Answer

A)  noncoding regions 
B)  introns 
C)  cis elements 
D)  gene regulatory regions 
E)  all of the above 
A)  noncoding regions 
B)  introns 
C)  cis elements 
D)  gene regulatory regions 
E)  all of the above

Question 2

Cohesin complexes

Accepted Answer

A)  are comprised of several proteins, including SMC proteins 
B)  are important during mitosis 
C)  are similar in structure to condensins 
D)  are important to chromatid separation 
E)  all of the above 
A)  are comprised of several proteins, including SMC proteins 
B)  are important during mitosis 
C)  are similar in structure to condensins 
D)  are important to chromatid separation 
E)  all of the above

Question 3

The trp operon is like the lac operon in that

Accepted Answer

A)  both constitutively express a repressor 
B)  both are repressed in a metabolite-rich environment 
C)  both rely on attenuation for control of expression 
D)  both (
A)  and (
B)  are correct 
E)  all of the above are correct 
A)  both constitutively express a repressor 
B)  both are repressed in a metabolite-rich environment 
C)  both rely on attenuation for control of expression 
D)  both (
A)  and (
B)  are correct 
E)  all of the above are correct

Question 4

What type of modification is used to make CpG islands?

Accepted Answer

A)  acetylation of guanine 
B)  methylation of guanine 
C)  phosphorylation of guanine 
D)  acetylation of cytosine 
E)  methylation of cytosine 
A)  acetylation of guanine 
B)  methylation of guanine 
C)  phosphorylation of guanine 
D)  acetylation of cytosine 
E)  methylation of cytosine

Question 5

The trp operon differs from the lac operon in that

Accepted Answer

A)  trp is important for energy metabolism, but lac is not 
B)  trp has a leader sequence immediately upstream of the structural genes 
C)  trp has a leader sequence immediately upstream of the operator 
D)  trp has three structural genes and lac has five structural genes 
E)  trp has five structural genes and lac has one structural gene 
A)  trp is important for energy metabolism, but lac is not 
B)  trp has a leader sequence immediately upstream of the structural genes 
C)  trp has a leader sequence immediately upstream of the operator 
D)  trp has three structural genes and lac has five structural genes 
E)  trp has five structural genes and lac has one structural gene

Question 6

FMN participates in a riboswitch. When FMN is present
I. it binds the aptamer domain of the FMN riboswitch
II. it results in the formation of a terminator loop
III. it causes rho-dependent termination
IV. transcription proceeds

Accepted Answer

A)  I only 
B)  I and II 
C)  III and IV 
D)  I, II and III 
E)  II and IV 
A)  I only 
B)  I and II 
C)  III and IV 
D)  I, II and III 
E)  II and IV

Question 7

Consensus sequences

Accepted Answer

A)  occur in proteins 
B)  occur in DNA 
C)  are also called palindromes 
D)  are commonly found in exons 
E)  Both (
A)  and (
B)  are correct 
A)  occur in proteins 
B)  occur in DNA 
C)  are also called palindromes 
D)  are commonly found in exons 
E)  Both (
A)  and (
B)  are correct

Question 8

The TPP riboswitch (or THI element)

Accepted Answer

A)  sequesters the Shine-Dalgarno sequence under times of high energy 
B)  inhibits translation of TPP (thiamine pyrophosphokinase) 
C)  regulates gene expression by altering ribosome binding site conformation 
D)  interferes with initiation factor binding 
E)  all of the above 
A)  sequesters the Shine-Dalgarno sequence under times of high energy 
B)  inhibits translation of TPP (thiamine pyrophosphokinase) 
C)  regulates gene expression by altering ribosome binding site conformation 
D)  interferes with initiation factor binding 
E)  all of the above

Question 9

A self-cleaving ribozyme cleaves ___ resulting in prevention of ___.

Accepted Answer

A)  mRNA; translation 
B)  mRNA; transcription 
C)  tRNA; transcription 
D)  tRNA; translation 
E)  rRNA; translation 
A)  mRNA; translation 
B)  mRNA; transcription 
C)  tRNA; transcription 
D)  tRNA; translation 
E)  rRNA; translation

Question 10

Both C/EBP (CCAAT/enhancer-binding protein) and CREB (cyclicAMP response element-binding protein) have a leucine zipper binding motif. Both are general transcription factors that are activated in the cytosol and translocate into the nucleus

Accepted Answer

A)  Both sentences are true 
B)  Both sentences are false 
C)  The first sentence is true and the second sentence is false 
D)  The first sentence is false and the second sentence is true 
A)  Both sentences are true 
B)  Both sentences are false 
C)  The first sentence is true and the second sentence is false 
D)  The first sentence is false and the second sentence is true

Question 11

Responsible for proliferation and differentiation, this family of transcription factors has a relatively large DNA binding domain.

Accepted Answer

A)  leucine zipper 
B)  homeodomain 
C)  POU domain 
D)  winged helix 
E)  zinc finger motif 
A)  leucine zipper 
B)  homeodomain 
C)  POU domain 
D)  winged helix 
E)  zinc finger motif

Question 12

Nuclear hormone receptors
I. recognize DNA segments that contain repeated sequences separated by several bases.
II. interact with DNA via two zinc finger domains, and also have a hinged region.
III. are highly conserved sequences found in prokaryotic and eukaryotic cells.

Accepted Answer

A)  I, II, III 
B)  I, II 
C)  II, III 
D)  I, III 
E)  II only 
A)  I, II, III 
B)  I, II 
C)  II, III 
D)  I, III 
E)  II only

Question 13

Which is NOT true of sterol regulatory element-binding protein (SREBP)?

Accepted Answer

A)  It is activated differently from most transcription factors 
B)  At low sterol hormone levels, it is bound to proteins in the ER membrane 
C)  One of its binding proteins acts as a sterol sensor 
D)  Still within the endomembrane system, its DNA binding domain is cleaved by proteases 
E)  Following translocation to the nucleus, it alters transcription 
A)  It is activated differently from most transcription factors 
B)  At low sterol hormone levels, it is bound to proteins in the ER membrane 
C)  One of its binding proteins acts as a sterol sensor 
D)  Still within the endomembrane system, its DNA binding domain is cleaved by proteases 
E)  Following translocation to the nucleus, it alters transcription

Question 14

cAMP receptor proteins (CRP or CAP)
I. interacts with DNA through a leucine zipper motif
II. forms dimeric structure binding 2 cAMP molecules
III. bound to cAMP, interacts with DNA upstream of the lac operon operator
IV. forms a bend in DNA to inhibit RNA polymerase binding

Accepted Answer

A)  I and II 
B)  II only 
C)  I, II and IV 
D)  II, III and IV 
E)  I, II, III and IV 
A)  I and II 
B)  II only 
C)  I, II and IV 
D)  II, III and IV 
E)  I, II, III and IV

Question 15

Typically, addition of a(n) _____ group to certain lysine residues of a histone protein makes the region of chromatin transcriptionally _____; addition of a(n) _____ group to certain lysine residues makes the region of chromatin transcriptionally _____.

Accepted Answer

A)  methyl; active; acetyl; active 
B)  acetyl; silent; methyl; active 
C)  phosphate; silent; methyl; silent 
D)  methyl; silent; acetyl; active 
E)  methyl; silent; phosphate; active 
A)  methyl; active; acetyl; active 
B)  acetyl; silent; methyl; active 
C)  phosphate; silent; methyl; silent 
D)  methyl; silent; acetyl; active 
E)  methyl; silent; phosphate; active

Question 16

Which is TRUE of structural maintenance of chromosome (SMC) proteins?
I. they help organize chromatin at the level of 30 nm fiber and above
II. they have braided helical structures that are hinged
III. they have ATPase domains
IV. they don't have homologs in prokaryotes

Accepted Answer

A)  II only 
B)  II and III 
C)  I and IV 
D)  I, III and IV 
E)  I, II and III 
A)  II only 
B)  II and III 
C)  I and IV 
D)  I, III and IV 
E)  I, II and III

Question 17

In ___, gene expression is controlled by formation of complex secondary RNA structures.

Accepted Answer

The answer of In ___, gene expression is controlled by...

Question 18

___ is a motif commonly found among steroid receptors and PPARs.

Accepted Answer

The answer of ___ is a motif commonly found among...

Question 19

The most prevalent mode of DNA-protein interaction is achieved with a(n) _____ that binds to the _____ groove of DNA.

Accepted Answer

A)  $\alpha$-helix; major 
B)  $\alpha$-helix; minor 
C)  $\beta$-sheet; major 
D)  $\beta$-sheet; minor 
E)  $\beta$-turn; major 
A)  $\alpha$-helix; major 
B)  $\alpha$-helix; minor 
C)  $\beta$-sheet; major 
D)  $\beta$-sheet; minor 
E)  $\beta$-turn; major

Question 20

In eukaryotes, DNA is organized into "beads on a string," or ____, which are further organized into structures called ___.

Accepted Answer

nucleosome

Exam 17: Control of Gene

Most commonly, proteins interact with DNA at

Cohesin complexes

The trp operon is like the lac operon in that

What type of modification is used to make CpG islands?

The trp operon differs from the lac operon in that

FMN participates in a riboswitch. When FMN is present I. it binds the aptamer domain of the FMN riboswitch II. it results in the formation of a terminator loop III. it causes rho-dependent termination IV. transcription proceeds

Consensus sequences

The TPP riboswitch (or THI element)

A self-cleaving ribozyme cleaves ___ resulting in prevention of ___.

Both C/EBP (CCAAT/enhancer-binding protein) and CREB (cyclicAMP response element-binding protein) have a leucine zipper binding motif. Both are general transcription factors that are activated in the cytosol and translocate into the nucleus

Responsible for proliferation and differentiation, this family of transcription factors has a relatively large DNA binding domain.

Nuclear hormone receptors I. recognize DNA segments that contain repeated sequences separated by several bases. II. interact with DNA via two zinc finger domains, and also have a hinged region. III. are highly conserved sequences found in prokaryotic and eukaryotic cells.

Which is NOT true of sterol regulatory element-binding protein (SREBP)?

cAMP receptor proteins (CRP or CAP) I. interacts with DNA through a leucine zipper motif II. forms dimeric structure binding 2 cAMP molecules III. bound to cAMP, interacts with DNA upstream of the lac operon operator IV. forms a bend in DNA to inhibit RNA polymerase binding

Typically, addition of a(n) _____ group to certain lysine residues of a histone protein makes the region of chromatin transcriptionally _____; addition of a(n) _____ group to certain lysine residues makes the region of chromatin transcriptionally _____.

Which is TRUE of structural maintenance of chromosome (SMC) proteins? I. they help organize chromatin at the level of 30 nm fiber and above II. they have braided helical structures that are hinged III. they have ATPase domains IV. they don't have homologs in prokaryotes

In ___, gene expression is controlled by formation of complex secondary RNA structures.

___ is a motif commonly found among steroid receptors and PPARs.

The most prevalent mode of DNA-protein interaction is achieved with a(n) _____ that binds to the _____ groove of DNA.

In eukaryotes, DNA is organized into "beads on a string," or ____, which are further organized into structures called ___.

The Chemical Foundations of Biochemistry

Nucleic Acids

Proteins I

Proteins Iienzymes

Membranes and an Introduction to Signal Transduction

Carbohydrates I

The Common Catabolic Pathway

Carbohydrates II

Lipids I

Lipids II

Amine Metabolism

Metabolism Integration

Nucleotide Deoxynucleotide Metabolism

Dna Replication, Damage and Repair

Rna Synthesis

Protein

Determination of Macromolecular Structure

Allosterism and Receptor-Ligand

Designer Proteins and Protein Folding

Biomolecule Purification

Bioinformatics and Omics

Signal

Protein Trafficking

Photosynthesis Nitrogen

Filters

A self-cleaving ribozyme cleaves _ resulting in prevention of _.

Typically, addition of a(n) ___ group to certain lysine residues of a histone protein makes the region of chromatin transcriptionally _; addition of a(n) _ group to certain lysine residues makes the region of chromatin transcriptionally ___.

The most prevalent mode of DNA-protein interaction is achieved with a(n) _ that binds to the _ groove of DNA.

In eukaryotes, DNA is organized into "beads on a string," or __, which are further organized into structures called _.