Question 1

One of the way SNARE proteins can be tethered to lipid bilayers is through

Accepted Answer

A)  palmitoylation of cysteine residues 
B)  palmitoylation of asparagine residues 
C)  eicosapentanoylation of asparagine residues 
D)  cysteine residues bound to cholesterol 
E)  asparagine residues bound to mannose 
A)  palmitoylation of cysteine residues 
B)  palmitoylation of asparagine residues 
C)  eicosapentanoylation of asparagine residues 
D)  cysteine residues bound to cholesterol 
E)  asparagine residues bound to mannose

Question 2

Vesicular coating by COPI and COPII is energized by

Accepted Answer

A)  GTP hydrolysis 
B)  ATP hydrolysis 
C)  ADP hydrolysis 
D)  GDP ribosylation enzyme 
E)  mannose-6-phosphate hydrolysis 
A)  GTP hydrolysis 
B)  ATP hydrolysis 
C)  ADP hydrolysis 
D)  GDP ribosylation enzyme 
E)  mannose-6-phosphate hydrolysis

Question 3

When not needed to transport cargo, kinesin is

Accepted Answer

A)  found in the cytosol 
B)  bound to ADP 
C)  has light chains phosphorylated by AMP kinase 
D)  Both A and B 
E)  All of the above 
A)  found in the cytosol 
B)  bound to ADP 
C)  has light chains phosphorylated by AMP kinase 
D)  Both A and B 
E)  All of the above

Question 4

Common to both motor proteins and DNA and RNA polymerases is

Accepted Answer

A)  processivity 
B)  all are catalysts 
C)  all require GTP hydrolysis for energy 
D)  all generate motion 
E)  None of the above 
A)  processivity 
B)  all are catalysts 
C)  all require GTP hydrolysis for energy 
D)  all generate motion 
E)  None of the above

Question 5

To effect translation, ribosomal subunits coalesce around ___ in the ___ of the cell.

Accepted Answer

A)  mRNA; cytosol 
B)  mRNA; rough ER membrane 
C)  tRNA; rough ER membrane 
D)  mRNA; rough ER lumen 
E)  mRNA; smooth ER membrane 
A)  mRNA; cytosol 
B)  mRNA; rough ER membrane 
C)  tRNA; rough ER membrane 
D)  mRNA; rough ER lumen 
E)  mRNA; smooth ER membrane

Question 6

Dynamin is associated with

Accepted Answer

A)  clathrin 
B)  vesicle formation 
C)  endocytosis 
D)  Both A and B 
E)  All of the above 
A)  clathrin 
B)  vesicle formation 
C)  endocytosis 
D)  Both A and B 
E)  All of the above

Question 7

Which inactivates vesicular coating and transport?

Accepted Answer

A)  ARF 
B)  GAP 
C)  GDI 
D)  GEF 
E)  None of the above 
A)  ARF 
B)  GAP 
C)  GDI 
D)  GEF 
E)  None of the above

Question 8

In the first step of vesicular binding of neurotransmitter vesicles to neuronal plasma membrane

Accepted Answer

A)  synaptobrevin associated with the plasma membrane interacts with SNAP-25 and syntaxin of the vesicle 
B)  SNAP-25 and syntaxin associated with the plasma membrane interacts with synaptobrevin of the vesicle 
C)  SNAP-25 associated with the plasma membrane interacts with synaptobrevin and syntaxin of the vesicle 
A)  synaptobrevin associated with the plasma membrane interacts with SNAP-25 and syntaxin of the vesicle 
B)  SNAP-25 and syntaxin associated with the plasma membrane interacts with synaptobrevin of the vesicle 
C)  SNAP-25 associated with the plasma membrane interacts with synaptobrevin and syntaxin of the vesicle

Question 9

As proteins move through the Golgi, a common post-translational modification is glycosylation. Other modifications include phosphorylation and sulfation.

Accepted Answer

A)  Both sentences are true 
B)  Both sentences are false 
C)  The first sentence is true and the second sentence is false 
D)  The first sentence is false and the second sentence is true 
A)  Both sentences are true 
B)  Both sentences are false 
C)  The first sentence is true and the second sentence is false 
D)  The first sentence is false and the second sentence is true

Question 10

Vesicles coated in COPI proteins depend on ADP-ribosylation factor (ARF), which is similar to ___ in COPII-coated vesicles.

Accepted Answer

A)  Rabs 
B)  GAP 
C)  GEF 
D)  Sar1 
E)  GDI 
A)  Rabs 
B)  GAP 
C)  GEF 
D)  Sar1 
E)  GDI

Question 11

Sar1 has

Accepted Answer

A)  GTPase activity 
B)  ATPase activity 
C)  kinase activity 
D)  phosphatase activity 
E)  None of the above 
A)  GTPase activity 
B)  ATPase activity 
C)  kinase activity 
D)  phosphatase activity 
E)  None of the above

Question 12

In motor proteins, the ___ determines the length of the "step" along the ___ .

Accepted Answer

A)  availability of ATP; cytoskeletal element 
B)  availability of ATP; plasma membrane 
C)  availiability of GTP; cytoskeletal element 
D)  length of the arm; cytoskeletal element 
E)  length of the arm; organellar membrane 
A)  availability of ATP; cytoskeletal element 
B)  availability of ATP; plasma membrane 
C)  availiability of GTP; cytoskeletal element 
D)  length of the arm; cytoskeletal element 
E)  length of the arm; organellar membrane

Question 13

Movement of very large vesicles and even organelles around the cell is mostly by

Accepted Answer

A)  dynein 
B)  kinesins 
C)  myosin V 
D)  myosin II 
E)  All of the above 
A)  dynein 
B)  kinesins 
C)  myosin V 
D)  myosin II 
E)  All of the above

Question 14

Addition of mannose-6-phosphate targets a protein to

Accepted Answer

A)  peroxisomes 
B)  the smooth ER 
C)  lysosomes 
D)  the mitochondrial inner membrane 
E)  the chloroplast thylakoid membrane 
A)  peroxisomes 
B)  the smooth ER 
C)  lysosomes 
D)  the mitochondrial inner membrane 
E)  the chloroplast thylakoid membrane

Question 15

Proteins acted upon by signal peptidase

Accepted Answer

A)  are destined to be further processed within the ER lumen 
B)  travel within a vesicle to the cis Golgi 
C)  retain the KDEL sequence 
D)  Both A and B are correct 
E)  All of the above are correct 
A)  are destined to be further processed within the ER lumen 
B)  travel within a vesicle to the cis Golgi 
C)  retain the KDEL sequence 
D)  Both A and B are correct 
E)  All of the above are correct

Question 16

Calcium must be present for muscle contraction to occur. Calcium

Accepted Answer

A)  fits in the binding sites of actin and attracts the myosin heads 
B)  fits in the binding sites of actin and attracts the myosin tails 
C)  binds troponin, causing tropomyosin to shift and expose actin binding sites 
D)  binds tropomyosin, causing troponin to shift and expose actin binding sites 
E)  serves as a cofactor in the catalytic subunit of myosin, encouraging ATP hydrolysis 
A)  fits in the binding sites of actin and attracts the myosin heads 
B)  fits in the binding sites of actin and attracts the myosin tails 
C)  binds troponin, causing tropomyosin to shift and expose actin binding sites 
D)  binds tropomyosin, causing troponin to shift and expose actin binding sites 
E)  serves as a cofactor in the catalytic subunit of myosin, encouraging ATP hydrolysis

Question 17

How do proteins move through the Golgi?

Accepted Answer

A)  From cis to cisternae to trans 
B)  From cisternae to trans to cis 
C)  From trans to cisternae to cis 
D)  anterograde to retrograde 
E)  retrograde to anterograde 
A)  From cis to cisternae to trans 
B)  From cisternae to trans to cis 
C)  From trans to cisternae to cis 
D)  anterograde to retrograde 
E)  retrograde to anterograde

Question 18

Myosin II's coiled-coil domain

Accepted Answer

A)  makes up the protein's head region 
B)  is necessary for ATP binding 
C)  serves as the ATPase catalytic subunit 
D)  binds calcium and regulates the protein's activity 
E)  makes up its tail 
A)  makes up the protein's head region 
B)  is necessary for ATP binding 
C)  serves as the ATPase catalytic subunit 
D)  binds calcium and regulates the protein's activity 
E)  makes up its tail

Question 19

For successful vesicular fusion in neuronal axons, ___ senses calcium availability.

Accepted Answer

The answer of For successful vesicular fusion in neuronal axons,...

Question 20

Although not completely understood, Myosin II's light chains apparently

Accepted Answer

A)  bind ATP 
B)  are responsible for hydrolyzing ATP 
C)  stabilize the head region 
D)  have a regulatory function 
E)  Both A and B are correct 
A)  bind ATP 
B)  are responsible for hydrolyzing ATP 
C)  stabilize the head region 
D)  have a regulatory function 
E)  Both A and B are correct

Exam 24: Protein Trafficking

One of the way SNARE proteins can be tethered to lipid bilayers is through

Vesicular coating by COPI and COPII is energized by

When not needed to transport cargo, kinesin is

Common to both motor proteins and DNA and RNA polymerases is

To effect translation, ribosomal subunits coalesce around ___ in the ___ of the cell.

Dynamin is associated with

Which inactivates vesicular coating and transport?

In the first step of vesicular binding of neurotransmitter vesicles to neuronal plasma membrane

As proteins move through the Golgi, a common post-translational modification is glycosylation. Other modifications include phosphorylation and sulfation.

Vesicles coated in COPI proteins depend on ADP-ribosylation factor (ARF), which is similar to ___ in COPII-coated vesicles.

Sar1 has

In motor proteins, the ___ determines the length of the "step" along the ___ .

Movement of very large vesicles and even organelles around the cell is mostly by

Addition of mannose-6-phosphate targets a protein to

Proteins acted upon by signal peptidase

Calcium must be present for muscle contraction to occur. Calcium

How do proteins move through the Golgi?

Myosin II's coiled-coil domain

For successful vesicular fusion in neuronal axons, ___ senses calcium availability.

Although not completely understood, Myosin II's light chains apparently

The Chemical Foundations of Biochemistry

Nucleic Acids

Proteins I

Proteins Iienzymes

Membranes and an Introduction to Signal Transduction

Carbohydrates I

The Common Catabolic Pathway

Carbohydrates II

Lipids I

Lipids II

Amine Metabolism

Metabolism Integration

Nucleotide Deoxynucleotide Metabolism

Dna Replication, Damage and Repair

Rna Synthesis

Protein

Control of Gene

Determination of Macromolecular Structure

Allosterism and Receptor-Ligand

Designer Proteins and Protein Folding

Biomolecule Purification

Bioinformatics and Omics

Signal

Photosynthesis Nitrogen

Filters

To effect translation, ribosomal subunits coalesce around _ in the _ of the cell.

In motor proteins, the _ determines the length of the "step" along the _ .