Exam 8: Antibody Detection and Identification

MATCHING Match the tentative interpretation of antibody screen and direct antiglobulin test (DAT) with the results given below. -All screening cells 2+ at antihuman globulin phase,DAT positive,IgG 2+

Anti-Fyᵃ was identified in a patient's serum.The patient's red cells phenotyped as Fy(a+)using commercial antisera.What is the next step?

What is the next step in the investigation of a positive direct antiglobulin test with polyspecific antihuman globulin reagent?

What is the typical specificity of cold autoantibodies?

Antibody screening cells are positive at the antihuman globulin phase of testing.What is the first step in this investigation?

A patient's serum reacted weakly with all panel cells tested at the antiglobulin phase using LISS and were not enhanced using PEG.The autocontrol was negative.Ficin-treated panel cells were nonreactive.What is the most likely specificity of the antibody?

Anti-D,anti-K,and anti-Jkᵃ are the antibodies that are tentatively identified on a panel after initially ruling out on negative cells.What selected cell from another panel should be chosen to confirm the presence of anti-K?

Which of the following situations can be found in a classic case of autoimmune hemolytic anemia?

In the process of identifying an antibody,the technologist observed 2+ reactions with 3 of the 10 cells in a panel at the immediate-spin phase.These reactions disappeared following incubation at 37° C and the antihuman globulin phase of testing.What is the most likely responsible antibody?

Why are proteolytic enzymes not used in the routine screening for antibodies?