Question 1

Which of the following is used in the production of recombinant DNA (rDNA)?

Accepted Answer

A)  vectors 
B)  restriction enzymes 
C)  DNA ligase 
D)  plasmids 
E)  All of the answer choices are used in the production of recombinant DNA. 
A)  vectors 
B)  restriction enzymes 
C)  DNA ligase 
D)  plasmids 
E)  All of the answer choices are used in the production of recombinant DNA.

Question 2

Ex vivo gene therapy

Accepted Answer

A)  requires the removal of cells from the individual undergoing the treatment and then the reintroduction of these cells once they have been genetically modified. 
B)  requires the use of an agent to introduce genetic material directly into the body using a vector such as an adenovirus vector. 
C)  has few or no side effects on the individual receiving the treatment. 
D)  allows for a wider range of disorders to be treated than in vivo treatments. 
E)  is considered to be more dangerous for the patient than in vivo treatment. 
A)  requires the removal of cells from the individual undergoing the treatment and then the reintroduction of these cells once they have been genetically modified. 
B)  requires the use of an agent to introduce genetic material directly into the body using a vector such as an adenovirus vector. 
C)  has few or no side effects on the individual receiving the treatment. 
D)  allows for a wider range of disorders to be treated than in vivo treatments. 
E)  is considered to be more dangerous for the patient than in vivo treatment.

Question 3

Transgenic crop plants have been created for which of the following reasons?

Accepted Answer

A)  to increase productivity 
B)  to increase resistance to insect damage 
C)  to create plants that are resistant to herbicides 
D)  to produce human hormones or antibodies 
E)  Transgenic plants have been produced to do all of the things listed. 
A)  to increase productivity 
B)  to increase resistance to insect damage 
C)  to create plants that are resistant to herbicides 
D)  to produce human hormones or antibodies 
E)  Transgenic plants have been produced to do all of the things listed.

Question 4

What is the role of DNA ligase in recombinant DNA technology?

Accepted Answer

A)  DNA ligase cleaves the vector DNA. 
B)  DNA ligase makes the bacterial cell more permeable to vector. 
C)  DNA ligase forms the plasmids that are used as vectors. 
D)  DNA ligase seals DNA into an opening created by a restriction enzyme. 
E)  DNA ligase acts as molecular scissors, cutting DNA apart at specific locations. 
A)  DNA ligase cleaves the vector DNA. 
B)  DNA ligase makes the bacterial cell more permeable to vector. 
C)  DNA ligase forms the plasmids that are used as vectors. 
D)  DNA ligase seals DNA into an opening created by a restriction enzyme. 
E)  DNA ligase acts as molecular scissors, cutting DNA apart at specific locations.

Question 5

DNA fingerprinting can be used for which of the following?

Accepted Answer

A)  paternity tests 
B)  authenticating Super Bowl footballs 
C)  forensic analysis 
D)  determining a predisposition to cancer 
E)  All of the answer choices are uses of DNA fingerprinting. 
A)  paternity tests 
B)  authenticating Super Bowl footballs 
C)  forensic analysis 
D)  determining a predisposition to cancer 
E)  All of the answer choices are uses of DNA fingerprinting.

Question 6

Which of the following is part of the process for PCR (polymerase chain reaction)?

Accepted Answer

A)  the use of an engineered DNA polymerase 
B)  the denaturing of the double-stranded DNA to yield single-strand DNA 
C)  multiple cycles of the chain reaction to yield a greater number of DNA copies 
D)  final product is double-stranded DNA 
E)  All of the answer choices are parts of the process for PCR. 
A)  the use of an engineered DNA polymerase 
B)  the denaturing of the double-stranded DNA to yield single-strand DNA 
C)  multiple cycles of the chain reaction to yield a greater number of DNA copies 
D)  final product is double-stranded DNA 
E)  All of the answer choices are parts of the process for PCR.

Question 7

RNAi is believed to have evolved in eukaryotic organisms as a protection against certain types of viruses.

Accepted Answer

A) True 
 B)False

Question 8

The human genome project discovered many small regions of DNA that vary among individuals called single nucleotide polymorphisms (SNPs). Certain SNP patterns change an individual's susceptibility to disease and response to medical treatments. This knowledge has led to the idea

Accepted Answer

A)  of designer drugs that are made to match one specific individual's DNA profile. 
B)  of designer babies that have known genetic traits. 
C)  that everyone will respond to a drug in the same way. 
D)  that not all humans are as well suited for survival. 
E)  that some DNA does not code for proteins. 
A)  of designer drugs that are made to match one specific individual's DNA profile. 
B)  of designer babies that have known genetic traits. 
C)  that everyone will respond to a drug in the same way. 
D)  that not all humans are as well suited for survival. 
E)  that some DNA does not code for proteins.

Question 9

Restriction enzymes

Accepted Answer

A)  exist in bacteria to restrict the growth of viruses. 
B)  cut double-stranded DNA at specific sites. 
C)  produce "sticky ends" that can bind foreign DNA. 
D)  facilitate inserting foreign DNA into vector DNA. 
E)  All of the answer choices describe restriction enzymes. 
A)  exist in bacteria to restrict the growth of viruses. 
B)  cut double-stranded DNA at specific sites. 
C)  produce "sticky ends" that can bind foreign DNA. 
D)  facilitate inserting foreign DNA into vector DNA. 
E)  All of the answer choices describe restriction enzymes.

Question 10

The genome of humans and fruit flies are approximately the same size and composed of the same number of genes.

Accepted Answer

A) True 
 B)False

Question 11

Enzymes from the thermophile organism Thermus aquaticus have allowed the development of which of the following procedures?

Accepted Answer

A)  genetic engineering 
B)  recombinant DNA 
C)  DNA cloning 
D)  polymerase chain reaction 
E)  protein synthesis 
A)  genetic engineering 
B)  recombinant DNA 
C)  DNA cloning 
D)  polymerase chain reaction 
E)  protein synthesis

Question 12

Bovine growth hormone is used to produce larger

Accepted Answer

A)  cows 
B)  fish 
C)  pigs 
D)  sheep 
E)  All of the answer choices are correct. 
A)  cows 
B)  fish 
C)  pigs 
D)  sheep 
E)  All of the answer choices are correct.

Question 13

A major difference in the production of transgenic bacteria, plants, and animals is that

Accepted Answer

A)  transgenic bacteria do not pass on their genetic modification to offspring, while plants and animals do. 
B)  transgenic animals require the use of a host to carry the genetically modified embryo, while bacteria and plants do not. 
C)  transgenic bacteria and plants receive genes from other species of organisms, while animals are only able to incorporate genes from their own species. 
D)  so far, only transgenic plants and animals have been successful. Scientists are still trying to create transgenic bacteria. 
E)  transgenic bacteria, animals, and plants can only incorporate genes from their own species, so the process is unique for each species. 
A)  transgenic bacteria do not pass on their genetic modification to offspring, while plants and animals do. 
B)  transgenic animals require the use of a host to carry the genetically modified embryo, while bacteria and plants do not. 
C)  transgenic bacteria and plants receive genes from other species of organisms, while animals are only able to incorporate genes from their own species. 
D)  so far, only transgenic plants and animals have been successful. Scientists are still trying to create transgenic bacteria. 
E)  transgenic bacteria, animals, and plants can only incorporate genes from their own species, so the process is unique for each species.

Question 14

Gene pharming is

Accepted Answer

A)  the use of bacterial DNA to impart drug resistance to plants. 
B)  the use of transgenic farm animals to produce pharmaceuticals. 
C)  the creation of new genes to be implanted in farm animals. 
D)  the creation of crop plants to replace the use of farm animals as a source of protein. 
E)  the application of genetic principles to breeding farm animals. 
A)  the use of bacterial DNA to impart drug resistance to plants. 
B)  the use of transgenic farm animals to produce pharmaceuticals. 
C)  the creation of new genes to be implanted in farm animals. 
D)  the creation of crop plants to replace the use of farm animals as a source of protein. 
E)  the application of genetic principles to breeding farm animals.

Question 15

When introducing foreign DNA into vector DNA, which of the following is necessary to seal DNA into an opening created by the restriction enzyme?

Accepted Answer

A)  DNA ligase 
B)  DNA polymerase 
C)  plasmids 
D)  RNA primase 
E)  DNA helicase 
A)  DNA ligase 
B)  DNA polymerase 
C)  plasmids 
D)  RNA primase 
E)  DNA helicase

Question 16

The purpose of PCR is to create

Accepted Answer

A)  fragments of DNA that are different lengths. 
B)  recombinant DNA. 
C)  billions of copies of a segment of DNA. 
D)  plasmids to be used as vectors in cloning. 
E)  transgenic crop plants. 
A)  fragments of DNA that are different lengths. 
B)  recombinant DNA. 
C)  billions of copies of a segment of DNA. 
D)  plasmids to be used as vectors in cloning. 
E)  transgenic crop plants.

Question 17

The treatment of a disorder by inserting genetic material into an organism is called

Accepted Answer

A)  karyotyping. 
B)  amniocentesis. 
C)  genetic profiling. 
D)  chorionic villi sampling. 
E)  gene therapy. 
A)  karyotyping. 
B)  amniocentesis. 
C)  genetic profiling. 
D)  chorionic villi sampling. 
E)  gene therapy.

Question 18

Which of the following is a concern over the use of genetically engineered corn?

Accepted Answer

A)  resistance among populations of certain pests 
B)  genetic exchange between engineered plants and related species 
C)  impact of engineered plants on nontarget plant species 
D)  presence of allergens 
E)  All of the answer choices describe concerns over the use of genetically engineered corn. 
A)  resistance among populations of certain pests 
B)  genetic exchange between engineered plants and related species 
C)  impact of engineered plants on nontarget plant species 
D)  presence of allergens 
E)  All of the answer choices describe concerns over the use of genetically engineered corn.

Exam 26: Biotechnology and Genomics

Which of the following is used in the production of recombinant DNA (rDNA)?

Ex vivo gene therapy

Transgenic crop plants have been created for which of the following reasons?

What is the role of DNA ligase in recombinant DNA technology?

DNA fingerprinting can be used for which of the following?

Which of the following is part of the process for PCR (polymerase chain reaction)?

RNAi is believed to have evolved in eukaryotic organisms as a protection against certain types of viruses.

The human genome project discovered many small regions of DNA that vary among individuals called single nucleotide polymorphisms (SNPs). Certain SNP patterns change an individual's susceptibility to disease and response to medical treatments. This knowledge has led to the idea

Restriction enzymes

The genome of humans and fruit flies are approximately the same size and composed of the same number of genes.

Enzymes from the thermophile organism Thermus aquaticus have allowed the development of which of the following procedures?

Bovine growth hormone is used to produce larger

A major difference in the production of transgenic bacteria, plants, and animals is that

Gene pharming is

When introducing foreign DNA into vector DNA, which of the following is necessary to seal DNA into an opening created by the restriction enzyme?

The purpose of PCR is to create

The treatment of a disorder by inserting genetic material into an organism is called

Which of the following is a concern over the use of genetically engineered corn?

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