Exam 15: Regulation of Gene Expression

Please use the following information to answer the question(s) below. Suppose an experimenter becomes proficient with a technique that allows her to move DNA sequences within a prokaryotic genome. -A researcher found a method she could use to manipulate and quantify phosphorylation and methylation in embryonic cells in culture. In one set of experiments she succeeded in decreasing methylation of histone tails. Which of the following results would she most likely see?

Which of the following is a protein product of a regulatory gene?

Which of the following mechanisms is used to coordinate the expression of multiple, related genes in eukaryotic cells?

Which of the following techniques involves reverse transcriptase, PCR amplification, and gel electrophoresis?

The functioning of enhancers is an example of

Which of the following would not be true of cDNA produced using human brain tissue as the starting material?

Please use the following information to answer the question(s) below. Suppose an experimenter becomes proficient with a technique that allows her to move DNA sequences within a prokaryotic genome. -A researcher introduces double-stranded RNA into a culture of mammalian cells. The double-stranded RNA corresponds to a region of a protein-coding gene of interest. How may the introduced RNA affect gene expression in these cells?

Most repressor proteins are allosteric. Which of the following molecules binds with the repressor to alter its structure?

If a particular operon encodes enzymes for making an essential amino acid and is regulated like the trp operon, then

Transcription of the genes in an inducible operon

What would occur if the repressor of an inducible operon were mutated so it could not bind the operator?

At the beginning of this century there was a general announcement regarding the sequencing of the human genome and the genomes of many other multicellular eukaryotes. There was surprise expressed by many that the number of protein-coding sequences in the human genome was much smaller than had been expected. Which of the following could account for most of the rest of the DNA in the human genome?

Please use the following information to answer the question(s) below. Suppose an experimenter becomes proficient with a technique that allows her to move DNA sequences within a prokaryotic genome. -A researcher found a method she could use to manipulate and quantify methylation of DNA in embryonic cells in culture. One of her colleagues suggested she try increased methylation of C nucleotides in a mammalian system. Which of the following results would she most likely see?

How have DNA microarrays had such a huge impact on genomic studies?

Please use the following information to answer the question(s) below. Suppose an experimenter becomes proficient with a technique that allows her to move DNA sequences within a prokaryotic genome. -A researcher found a method she could use to manipulate and quantify acetylation and methylation in embryonic cells in culture. In one set of experiments using this procedure in Drosophila, she was readily successful in increasing acetylation of amino acids adjacent to methylated amino acids in histone tails. Which of the following results would she most likely see?

Among the newly discovered small noncoding RNAs, one type reestablishes methylation patterns during gamete formation and blocks expression of some transposons. These RNAs are called

Gene expression might be altered based on post-transcriptional processing in eukaryotes rather than prokaryotes because of which of the following?

When DNA is compacted by histones into 10-nm and 30-nm fibers, the DNA is unable to interact with proteins required for gene expression. Therefore, to allow for these proteins to act, the chromatin must constantly alter its structure. Which processes contribute to this dynamic activity?

Which of the following environments would most likely lead to the transcription of the lactose operon?

In a genome-wide expression study using a DNA microarray assay, what is each well used to detect?