Question 1

Molecular biologists use restriction enzymes to amplify a specific section of DNA within the genome.

Accepted Answer

A) True 
 B)False  False

Question 2

A vector is a small segment of DNA that a gene of interest is inserted into for cloning.

Accepted Answer

A) True 
 B)False  True

Question 3

Hematopoietic stem cells are ________.

Accepted Answer

A)  pluripotent 
B)  totipotent 
C)  unipotent 
D)  multipotent 
A)  pluripotent 
B)  totipotent 
C)  unipotent 
D)  multipotent D

Question 4

Since cDNA is made from RNA,it lacks introns and contains only the coding sequence for the functional protein.

Accepted Answer

A) True 
 B)False

Question 5

The use of fluorescent-labeled dideoxynucleotides is a characteristic of what type of sequencing?

Accepted Answer

A)  automated DNA sequencing 
B)  base-specific DNA cleavage 
A)  automated DNA sequencing 
B)  base-specific DNA cleavage

Question 6

Which of the following cells is not pluripotent?

Accepted Answer

A)  embryonic stem cells 
B)  embryonic germ cells 
C)  both are pluripotent 
D)  neither are pluripotent 
A)  embryonic stem cells 
B)  embryonic germ cells 
C)  both are pluripotent 
D)  neither are pluripotent

Question 7

The primary interest of genetic research into stem cells is the desire to clone a human being.

Accepted Answer

A) True 
 B)False

Question 8

The isolation and copying of a gene,usually in large quantities is called gene cloning.

Accepted Answer

A) True 
 B)False

Question 9

The origins of which of the following cell types creates the least amount of ethical debate?

Accepted Answer

A)  embryonic germ cells 
B)  hematopoietic stem cells 
C)  embryonic stem cells 
D)  All of the answers are equal. 
A)  embryonic germ cells 
B)  hematopoietic stem cells 
C)  embryonic stem cells 
D)  All of the answers are equal.

Question 10

What is the origin of restriction endonucleases?

Accepted Answer

A)  They are part of DNA repair mechanisms in eukaryotic cells. 
B)  They are a defense mechanism against viruses in bacteria. 
C)  They are replication enzymes of yeast. 
D)  They are transposable elements of Drosophila. 
A)  They are part of DNA repair mechanisms in eukaryotic cells. 
B)  They are a defense mechanism against viruses in bacteria. 
C)  They are replication enzymes of yeast. 
D)  They are transposable elements of Drosophila.

Question 11

Select items that describe a stage of a PCR reaction.Choose all that apply.

Accepted Answer

A)  DNA is heated to a temperature that causes the strands to separate. 
B)  Primers bind to complementary sequences on the template DNA. 
C)  DNA gyrase enzyme relieves the supercoiling ahead of the replication fork. 
D)  The DNA polymerase synthesizes a new strand of DNA that is complementary to the template DNA. 
A)  DNA is heated to a temperature that causes the strands to separate. 
B)  Primers bind to complementary sequences on the template DNA. 
C)  DNA gyrase enzyme relieves the supercoiling ahead of the replication fork. 
D)  The DNA polymerase synthesizes a new strand of DNA that is complementary to the template DNA.

Question 12

In a cloning experiment with chromosomal DNA,you use a vector that contains a lacZ gene as a selectable marker.If the competent cells are grown on X-Gal and IPTG,which colonies would contain cloned chromosomal DNA?

Accepted Answer

A)  the white colonies 
B)  the blue colonies 
C)  half the total colonies 
D)  none of the colonies 
A)  the white colonies 
B)  the blue colonies 
C)  half the total colonies 
D)  none of the colonies

Question 13

DNA ligase is needed in a cloning experiment for what reason?

Accepted Answer

A)  to proofread the vector for mistakes 
B)  to reestablish the stable sugar-phosphate backbone of the DNA molecule 
C)  to allow the vector to enter into the cell 
D)  as a selectable marker 
A)  to proofread the vector for mistakes 
B)  to reestablish the stable sugar-phosphate backbone of the DNA molecule 
C)  to allow the vector to enter into the cell 
D)  as a selectable marker

Question 14

Which procedure is used to identify a specific protein within a mixture of many different protein molecules?

Accepted Answer

A)  Western blotting 
B)  Northern blotting 
C)  Southern blotting 
D)  Eastern blotting 
A)  Western blotting 
B)  Northern blotting 
C)  Southern blotting 
D)  Eastern blotting

Question 15

Antibodies can be used as a probe for which of the following techniques?

Accepted Answer

A)  Western blotting 
B)  Northern blotting 
C)  Southern blotting 
D)  Eastern blotting 
A)  Western blotting 
B)  Northern blotting 
C)  Southern blotting 
D)  Eastern blotting

Question 16

What is a property of stem cells?

Accepted Answer

A)  They can differentiate into one or more specialized cell types. 
B)  They can form any tissue in the body. 
C)  They are only capable of one to two cell divisions. 
D)  They will only form hematopoietic cells. 
A)  They can differentiate into one or more specialized cell types. 
B)  They can form any tissue in the body. 
C)  They are only capable of one to two cell divisions. 
D)  They will only form hematopoietic cells.

Question 17

The first organism ever cloned by researchers was Dolly the sheep in 1997.

Accepted Answer

A) True 
 B)False

Question 18

A plasmid that contains separate origins of replication for two different species is called a(n)________.

Accepted Answer

A)  R factor 
B)  expression vector 
C)  viral vector 
D)  shuttle vector 
A)  R factor 
B)  expression vector 
C)  viral vector 
D)  shuttle vector

Question 19

Which of the following individual(s)developed the process of the polymerase chain reaction?

Accepted Answer

A)  Watson and Crick 
B)  McClintock 
C)  Mullis 
D)  Pauling 
A)  Watson and Crick 
B)  McClintock 
C)  Mullis 
D)  Pauling

Question 20

What is a DNA library?

Accepted Answer

A)  a collection of vectors, each containing a fragment of the genome or DNA of interest 
B)  a complete sequence of the genetic material in an organism 
C)  an electronic file that may be used for additional genetic analysis 
D)  a collection of mRNA from a given cell 
A)  a collection of vectors, each containing a fragment of the genome or DNA of interest 
B)  a complete sequence of the genetic material in an organism 
C)  an electronic file that may be used for additional genetic analysis 
D)  a collection of mRNA from a given cell

Exam 20: Molecular Technologies

Molecular biologists use restriction enzymes to amplify a specific section of DNA within the genome.

A vector is a small segment of DNA that a gene of interest is inserted into for cloning.

Hematopoietic stem cells are ________.

Since cDNA is made from RNA,it lacks introns and contains only the coding sequence for the functional protein.

The use of fluorescent-labeled dideoxynucleotides is a characteristic of what type of sequencing?

Which of the following cells is not pluripotent?

The primary interest of genetic research into stem cells is the desire to clone a human being.

The isolation and copying of a gene,usually in large quantities is called gene cloning.

The origins of which of the following cell types creates the least amount of ethical debate?

What is the origin of restriction endonucleases?

Select items that describe a stage of a PCR reaction.Choose all that apply.

In a cloning experiment with chromosomal DNA,you use a vector that contains a lacZ gene as a selectable marker.If the competent cells are grown on X-Gal and IPTG,which colonies would contain cloned chromosomal DNA?

DNA ligase is needed in a cloning experiment for what reason?

Which procedure is used to identify a specific protein within a mixture of many different protein molecules?

Antibodies can be used as a probe for which of the following techniques?

What is a property of stem cells?

The first organism ever cloned by researchers was Dolly the sheep in 1997.

A plasmid that contains separate origins of replication for two different species is called a(n)________.

Which of the following individual(s)developed the process of the polymerase chain reaction?

What is a DNA library?

Overview of Genetics

Reproduction and Chromosome Transmission

Mendelian Inheritance

Sex Determination and Sex Chromosomes

Extensions of Mendelian Inheritance

Extranuclear Inheritance, Imprinting, and Maternal Effect

Genetic Linkage and Mapping in Eukaryotes

Variation in Chromosome Structure and Number

Genetics in Bacteria

Genetics of Viruses

Molecular Structure of DNA and RNA

Molecular Structure of Chromosomes and Transposition

Dna Replication and Recombination

Gene Transcription and RNA Modification

Translation of MRNA

Gene Regulation in Bacteria

Gene Regulation in Eukaryotes

Non-Coding Rnas

Gene Mutation and DNA Repair

Genomics

Medical Genetics and Cancer

Population Genetics

Quantitative Genetics

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