Question 1

Which of the following is an example of a processive enzyme?

Accepted Answer

A)  DNA polymerase I 
B)  DNA polymerase III 
C)  DNA ligase 
D)  Okazaki fragments 
A)  DNA polymerase I 
B)  DNA polymerase III 
C)  DNA ligase 
D)  Okazaki fragments B

Question 2

Select the reasons that DNA synthesis occurs with a high degree of fidelity.

Accepted Answer

A)  The hydrogen bonding between G and C or A and T is much more stable than between mismatched pairs. 
B)  The active site of DNA polymerase preferentially catalyzes the attachment of nucleotides when the correct bases are located in opposite strands. 
C)  DNA polymerase enzymatically removes mismatched nucleotides. 
D)  DNA polymerase has the ability to change the structure of the base in order to form the correct bond. 
A)  The hydrogen bonding between G and C or A and T is much more stable than between mismatched pairs. 
B)  The active site of DNA polymerase preferentially catalyzes the attachment of nucleotides when the correct bases are located in opposite strands. 
C)  DNA polymerase enzymatically removes mismatched nucleotides. 
D)  DNA polymerase has the ability to change the structure of the base in order to form the correct bond. A,B,C

Question 3

Based on their known functions,which of the enzymes in the following pairs play a similar role in prokaryotes and eukaryotes?

Accepted Answer

A)  DNA pol I : DNA pol α 
B)  DNA pol II : DNA pol β 
C)  DNA pol III : DNA pol γ 
A)  DNA pol I : DNA pol α 
B)  DNA pol II : DNA pol β 
C)  DNA pol III : DNA pol γ B

Question 4

The first generation of replication in the Meselson and Stahl experiment disproved which theory of replication?

Accepted Answer

A)  semiconservative 
B)  conservative 
C)  dispersive 
D)  None-it took more than one generation to disprove the theory. 
A)  semiconservative 
B)  conservative 
C)  dispersive 
D)  None-it took more than one generation to disprove the theory.

Question 5

Which enzyme fills in small regions of DNA where the RNA primers were located?

Accepted Answer

A)  DNA ligase 
B)  DNA primase 
C)  topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III 
A)  DNA ligase 
B)  DNA primase 
C)  topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III

Question 6

Which of the following may account for the process of gene conversion?

Accepted Answer

A)  DNA mismatch repair 
B)  DNA gap repair 
C)  DNA proofreading 
D)  Telomerase errors 
E)  Flap endonuclease 
A)  DNA mismatch repair 
B)  DNA gap repair 
C)  DNA proofreading 
D)  Telomerase errors 
E)  Flap endonuclease

Question 7

What functions are accomplished by the primosome?

Accepted Answer

A)  tracking along DNA 
B)  tracking along DNA, separating double-stranded DNA 
C)  tracking along DNA, separating double-stranded DNA, synthesizing RNA primers 
D)  tracking along DNA, separating double-stranded DNA, synthesizing RNA primers, adding nucleotides 
A)  tracking along DNA 
B)  tracking along DNA, separating double-stranded DNA 
C)  tracking along DNA, separating double-stranded DNA, synthesizing RNA primers 
D)  tracking along DNA, separating double-stranded DNA, synthesizing RNA primers, adding nucleotides

Question 8

Which enzyme synthesizes the lagging strand of the DNA?

Accepted Answer

A)  DNA ligase 
B)  DNA primase 
C)  Topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III 
A)  DNA ligase 
B)  DNA primase 
C)  Topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III

Question 9

A primosome consists of a polymerase and a single-stranded binding protein.

Accepted Answer

A) True 
 B)False

Question 10

The synthesis of the daughter strand of DNA occurs away from the replication fork in the leading strand.

Accepted Answer

A) True 
 B)False

Question 11

How many DNA polymerases are found in prokaryotes?

Accepted Answer

A)  5 
B)  7 
C)  9 
D)  12 
A)  5 
B)  7 
C)  9 
D)  12

Question 12

The purpose of DNA replication is to produce ________.

Accepted Answer

A)  two daughter strands 
B)  two parental strands 
C)  two template strands 
D)  four daughter strands 
A)  two daughter strands 
B)  two parental strands 
C)  two template strands 
D)  four daughter strands

Question 13

You have discovered a strain of E.coli that grows very slowly-the generation time is nearly 12 hours compared to the normal 20-30 minutes.Upon further investigation,you find a mutation in a gene for one of the subunits of DNA polymerase III.What subunit is this mutation most likely to be in?

Accepted Answer

A)  α 
B)  β 
C)  γ 
D)  δ 
A)  α 
B)  β 
C)  γ 
D)  δ

Question 14

What process does the Holliday model help explain?

Accepted Answer

A)  recombination between homologous chromosomes 
B)  recombination between sister chromatids 
C)  the creation of shortened chromosomes 
D)  replication initiation 
A)  recombination between homologous chromosomes 
B)  recombination between sister chromatids 
C)  the creation of shortened chromosomes 
D)  replication initiation

Question 15

The Meselson-Stahl experiments supported the model of dispersive DNA replication.

Accepted Answer

A) True 
 B)False

Question 16

The rate at which DNA polymerase III synthesizes a new strand of DNA is 300 nucleotides per minute.

Accepted Answer

A) True 
 B)False

Question 17

Which enzyme attaches adjacent Okazaki fragments,forming a continuous DNA strand?

Accepted Answer

A)  DNA ligase 
B)  DNA primase 
C)  topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III 
A)  DNA ligase 
B)  DNA primase 
C)  topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III

Question 18

The Meselson-Stahl experiments used ³⁵S radioisotopes to determine the mechanism of DNA replication.

Accepted Answer

A) True 
 B)False

Question 19

Which enzyme manufactures a 10-12 base segment of RNA?

Accepted Answer

A)  DNA ligase 
B)  DNA primase 
C)  topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III 
A)  DNA ligase 
B)  DNA primase 
C)  topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III

Question 20

Which describes the mechanism of DNA replication in which both parental strands remain together following replication?

Accepted Answer

A)  dispersive 
B)  semiconservative 
C)  conservative 
A)  dispersive 
B)  semiconservative 
C)  conservative

Exam 13: Dna Replication and Recombination

Which of the following is an example of a processive enzyme?

Select the reasons that DNA synthesis occurs with a high degree of fidelity.

Based on their known functions,which of the enzymes in the following pairs play a similar role in prokaryotes and eukaryotes?

The first generation of replication in the Meselson and Stahl experiment disproved which theory of replication?

Which enzyme fills in small regions of DNA where the RNA primers were located?

Which of the following may account for the process of gene conversion?

What functions are accomplished by the primosome?

Which enzyme synthesizes the lagging strand of the DNA?

A primosome consists of a polymerase and a single-stranded binding protein.

The synthesis of the daughter strand of DNA occurs away from the replication fork in the leading strand.

How many DNA polymerases are found in prokaryotes?

The purpose of DNA replication is to produce ________.

You have discovered a strain of E.coli that grows very slowly-the generation time is nearly 12 hours compared to the normal 20-30 minutes.Upon further investigation,you find a mutation in a gene for one of the subunits of DNA polymerase III.What subunit is this mutation most likely to be in?

What process does the Holliday model help explain?

The Meselson-Stahl experiments supported the model of dispersive DNA replication.

The rate at which DNA polymerase III synthesizes a new strand of DNA is 300 nucleotides per minute.

Which enzyme attaches adjacent Okazaki fragments,forming a continuous DNA strand?

The Meselson-Stahl experiments used ³⁵S radioisotopes to determine the mechanism of DNA replication.

Which enzyme manufactures a 10-12 base segment of RNA?

Which describes the mechanism of DNA replication in which both parental strands remain together following replication?

Overview of Genetics

Reproduction and Chromosome Transmission

Mendelian Inheritance

Sex Determination and Sex Chromosomes

Extensions of Mendelian Inheritance

Extranuclear Inheritance, Imprinting, and Maternal Effect

Genetic Linkage and Mapping in Eukaryotes

Variation in Chromosome Structure and Number

Genetics in Bacteria

Genetics of Viruses

Molecular Structure of DNA and RNA

Molecular Structure of Chromosomes and Transposition

Gene Transcription and RNA Modification

Translation of MRNA

Gene Regulation in Bacteria

Gene Regulation in Eukaryotes

Non-Coding Rnas

Gene Mutation and DNA Repair

Molecular Technologies

Genomics

Medical Genetics and Cancer

Population Genetics

Quantitative Genetics

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