Question 1

When conjugation occurs between a Hfr donor and a F⁻ recipient,which of the following describes the outcome

Accepted Answer

A) F⁺ cells 
B) F⁻ cells with some quantity of chromosomal DNA 
C) F⁻ cells with no chromosomal DNA 
D) F' cells with some duplicate gene pairs 
A) F⁺ cells 
B) F⁻ cells with some quantity of chromosomal DNA 
C) F⁻ cells with no chromosomal DNA 
D) F' cells with some duplicate gene pairs

Question 2

When genes are passed on from parent to offspring,which of the following events has taken place

Accepted Answer

A) horizontal gene transfer 
B) transduction 
C) vertical gene transfer 
D) retrograde gene transfer 
A) horizontal gene transfer 
B) transduction 
C) vertical gene transfer 
D) retrograde gene transfer

Question 3

In horizontal gene transfer:

Accepted Answer

A) genes are passed between bacteria of different generations 
B) genes are passed from mother cells to daughter cells 
C) genes are passed between bacteria of the same generation 
D) none of these 
A) genes are passed between bacteria of different generations 
B) genes are passed from mother cells to daughter cells 
C) genes are passed between bacteria of the same generation 
D) none of these

Question 4

Which of the following processes increases variation seen in bacteria

Accepted Answer

A) Transformation 
B) Conjugation 
C) Transduction 
D) All of these 
A) Transformation 
B) Conjugation 
C) Transduction 
D) All of these

Question 5

The process of conjugation in bacteria requires that:

Accepted Answer

A) two types of viruses be present 
B) the bacteria be deprived of nutrients 
C) there be naked DNA in the environment 
D) there be contact between donor and recipient cells 
A) two types of viruses be present 
B) the bacteria be deprived of nutrients 
C) there be naked DNA in the environment 
D) there be contact between donor and recipient cells

Question 6

In specialized transduction the bacterial genes transduced tend to be:

Accepted Answer

A) those genes in greatest use by the bacteria 
B) those genes that are currently not being transcribed 
C) those genes that are located close to the site of the prophage insertion 
D) those genes that are small enough to fit in the viral head 
A) those genes in greatest use by the bacteria 
B) those genes that are currently not being transcribed 
C) those genes that are located close to the site of the prophage insertion 
D) those genes that are small enough to fit in the viral head

Question 7

F' (prime)plasmids:

Accepted Answer

A) are responsible for high frequency recombination 
B) carry some chromosomal genes 
C) are those plasmids that have never been incorporated into a bacterial chromosome 
D) none of these 
A) are responsible for high frequency recombination 
B) carry some chromosomal genes 
C) are those plasmids that have never been incorporated into a bacterial chromosome 
D) none of these

Question 8

Restriction enzymes come from _____.

Accepted Answer

A) fungal cells 
B) viruses 
C) bacterial cells 
D) animal cells 
A) fungal cells 
B) viruses 
C) bacterial cells 
D) animal cells

Question 9

RFLP's are the result of _____.

Accepted Answer

A) treating cells with enzymes that remove their cell walls 
B) fusing two cells together 
C) genes being transferred by a virus 
D) cutting DNA with restriction enzymes 
A) treating cells with enzymes that remove their cell walls 
B) fusing two cells together 
C) genes being transferred by a virus 
D) cutting DNA with restriction enzymes

Question 10

High frequency recombination cells arise when:

Accepted Answer

A) They have multiple F plasmids 
B) A portion of the cell's F plasmid has been incorporated into the bacterial chromosome 
C) Conjugation and transformation happen at the same time 
D) Bacterial chromosomes do not break during conjugation 
A) They have multiple F plasmids 
B) A portion of the cell's F plasmid has been incorporated into the bacterial chromosome 
C) Conjugation and transformation happen at the same time 
D) Bacterial chromosomes do not break during conjugation

Question 11

A bacterium that is transformed:

Accepted Answer

A) cannot form a conjugation pilus 
B) has acquired naked DNA from the environment 
C) will probably die within 48 hours 
D) if injected,it always will result in the death of a mouse 
A) cannot form a conjugation pilus 
B) has acquired naked DNA from the environment 
C) will probably die within 48 hours 
D) if injected,it always will result in the death of a mouse

Question 12

A virus which incorporates its DNA into the DNA of its host organism is existing as a _____.

Accepted Answer

A) virulent phage 
B) prophage 
C) lytic phage 
D) none of these 
A) virulent phage 
B) prophage 
C) lytic phage 
D) none of these

Question 13

Hybridomas:

Accepted Answer

A) are responsible for tumor formation in plants 
B) are resistant to monoclonal antibodies 
C) divide and grow for only a short period of time 
D) are the fusion of antibody producing plasma cell and a cancer cell 
A) are responsible for tumor formation in plants 
B) are resistant to monoclonal antibodies 
C) divide and grow for only a short period of time 
D) are the fusion of antibody producing plasma cell and a cancer cell

Question 14

In 1981 a review of safety concerns concerning recombinant DNA research found that:

Accepted Answer

A) mutants of E.coli require special sanitization practices 
B) laboratory workers could become easily infected with the strain of E.coli normally used in lab experiments 
C) no illnesses could be traced to laboratory researchers working with recombinants 
D) two of these are true 
A) mutants of E.coli require special sanitization practices 
B) laboratory workers could become easily infected with the strain of E.coli normally used in lab experiments 
C) no illnesses could be traced to laboratory researchers working with recombinants 
D) two of these are true

Exam 9: Gene Transfer and Genetic Engineering

When conjugation occurs between a Hfr donor and a F⁻ recipient,which of the following describes the outcome

When genes are passed on from parent to offspring,which of the following events has taken place

In horizontal gene transfer:

Which of the following processes increases variation seen in bacteria

The process of conjugation in bacteria requires that:

In specialized transduction the bacterial genes transduced tend to be:

F' (prime)plasmids:

Restriction enzymes come from _____.

RFLP's are the result of _____.

High frequency recombination cells arise when:

A bacterium that is transformed:

A virus which incorporates its DNA into the DNA of its host organism is existing as a _____.

Hybridomas:

In 1981 a review of safety concerns concerning recombinant DNA research found that:

Scope and History of Microbiology

The Microbiome

Fundamentals of Chemistry

Microscopy and Staining

Characteristics of Prokaryotic and Eukaryotic Cells

Essential Concepts of Metabolism

Growth and Culturing of Bacteria

Microbial Genetics

An Introduction to Taxonomy: The Bacteria

Viruses

Eukaryotic Microorganisms and Parasites

Sterilization and Disinfection

Antimicrobial Therapy

Host-Microbe Relationships and Disease Processes

Epidemiology and Nosocomial Infections

Innate Host Defenses

Basic Principles of Adaptive Immunity and Immunization

Immune Disorders

Diseases of the Skin and Eyes; Wounds and Bites

Urogenital and Sexually Transmitted Diseases

Diseases of the Respiratory System

Oral and Gastrointestinal Diseases

Cardiovascular, Lymphatic, and Systemic Diseases

Diseases of the Nervous System

Environmental Microbiology

Applied Microbiology

Filters