Exam 11: Genetic Engineering and Biotechnology

Create an experiment where you would use both a Southern blot and a Northern blot to determine if a newly isolated bacterium contains and actively uses the same catabolic pathway as previously demonstrated.What is the value in doing the Southern blot first? With results from these experiments,how would you conclude if the same pathway is active or not?

Recognizing pathogens that contain multiple unique proteins which enable the human immune system to recognize just one and mount an effective response has opened the door on development of some vaccines only being

Which of the following is NOT a common step in creating a genomic library?

How can foreign DNA of greater than 300 kbp be inserted and stably maintained in BAC vectors?

When is it appropriate to use an artificial chromosome vector? Describe a specific example.

Explain how a DNA library is created and how a particular vector is selected.

A polymerase chain reaction (PCR)copies an individual gene segment in vitro with a(n)________ primer(s).

One method to circumvent issues with introns when expression eukaryotic gene is a bacterium is to simply clone the mature transcript.

Which of the following sequences would be cleaved by a type II restriction endonuclease?

A poorly immunogenic vaccine often suggests the foreign proteins were not properly recognized by the immune system due to a lack of ________ necessary,which can also be engineered to occur with additional molecular manipulations.

Defend why codon usage must be considered when cloning a gene into another organism.

If vaccinia viruses were not both immunogenic and relatively benign,they would likely not be a favored vehicle for vaccinations.

Compare how the process of cloning differs when a vector with sticky ends is used and when a vector with blunt ends is used.

Cloning vectors can be distinguished from expression vectors by

Which of those below is NOT an important consideration when designing a fusion protein construct?

Describe the three main steps to clone a gene into an organism.

Describe a method developed by molecular biologists to easily observe the success of a genetic engineering procedure involving the ligating of a gene of interest into a plasmid.

Type II restriction endonucleases

Compare and contrast operon and protein fusions.

Explain the process of site-directed mutagenesis,and discuss some applications of this technique.