Exam 2: Microbial Cell Structure and Function

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Photoreceptors are analogous to chemoreceptors in that they are both proteinaceous sensors.

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You have discovered a new microorganism,but you want to know if it is a eukaryote or a prokaryote.To investigate this question you prepare a slide with a simple stain and view it with a light microscope with a 40X objective lens and 10X ocular lens.You also prepare a control slide using Saccharomyces cerevisiae (a unicellular eukaryote).You can see the cells on your control slide,but you don't see cells when you look at your unknown microorganism.What can you conclude from this experiment?

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You are studying swimming motility in a pathogenic bacillus.You create mutations in random genes and then test which mutations effect swimming motility by looking at the mutant cells under the microscope.One of the mutant bacteria can not swim anymore,but still rotates around in a one spot when you watch them.Using electron microscopy you discover that some parts of the flagella are still present in the cell wall,but no long flagella are visible.Which gene do you think is mutated (i.e.,missing)and which motility-related parts are still present in this mutant?

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The flagellar apparatus is put together in a particular order first the MS ring,anchoring proteins,and the hook extend off of the cytoplasmic membrane.Flagellin proteins then pass through the narrow filament channel and cap proteins finally are put onto the end when roughly 20,000 flagellin have been assembled.Flagellar growth thus occurs after the assembly of the basal body,rings,and hook.The basal body provides the rotation and uses the proton motive force to rotate the basal body.From the information given above,one can infer that the basal body and hook can still rotate,but the flagellin proteins are missing or defective so that no long flagella can form.This would explain why the cells might still rotate in place,but not be able to move forward rapidly.

Chemotaxis is a sensory response affecting the rotational direction of the flagellar motor.

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You have discovered a new coccoid-shaped microorganism with no nucleus,a rigid cell wall,and a diameter of 2 µm.Chemical tests reveal that its cell wall does NOT contain peptidoglycan.The new microorganism is

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Porins are channels in the outer membranes of gram-positive Bacteria.

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Hydrolytic enzymes function in the

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The lipopolysaccharide (LPS)layer is found ONLY in the cell walls of

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Lysozyme is an enzyme that can ultimately lyse and kill eukaryotic cells by breaking β-1,4-glycosidic bonds in peptidoglycan.

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Teichoic acids are commonly found in gram-negative cell walls.

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Compare and contrast the chemical composition and structure of the cytoplasmic membranes found in Bacteria and Archaea.What is the advantage of the archaeal membranes in relationship to the types of environments many archaea inhabit?

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Explain why prokaryotes tend to survive and adapt more rapidly to extreme and dynamic environmental conditions than eukaryotes.

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Compared to Eukaryotes,Bacteria and Archaea have ________ surface-to-volume ratios,causing ________ nutrient exchange and growth rates.

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Which of the following statements is TRUE?

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Describe the mechanisms by which certain prokaryotes glide.What are the ecological advantages of gliding motility?

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An organism of the genus Staphylococcus is ________,while an organism of the genus Spirochaeta is ________.

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The rigid layer that is present in the cell walls of Bacteria that is primarily responsible for the strength of the wall is known as

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In general,most cell inclusions function as energy reserves or as a reservoir of structural building blocks.

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Elaborate on why discovering endospores was important to microbiology.

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Which of the following types of microscopy could be used to visualize the layers of the cell membrane and the cell wall?

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