Exam 11: Genetic Engineering and Biotechnology

Developing vaccines for humans relies heavily on manipulating and engineering vectors.

To estimate the total concentration of a beneficial bacterial species in yogurt,________ would provide the quickest results.

DNA ligase mediates the insertion of foreign DNA into a vector,but it will only be able to do so if the inserts and vector have matching sticky or blunt ends.

Describe the methodology employed when antibodies are used to detect proteins.

Inserting a kanamycin resistance cassette into a catabolic operon to confirm the gene is essential in degradation of a particular compound would involve all of the following EXCEPT

Due to well developed molecular tools and careful screening designs,functional genes can be isolated directly by isolation from the environment rather than cultivating the diverse species in a microbial community.

While other types exist,Type II restriction endonucleases are by far the most commonly used enzymes for genetic engineering.

When trying to make a mammalian protein in a bacterium,expression vectors are often used.Using your knowledge of prokaryotic and eukaryotic genetics,explain why expression vectors are so important.

Describe the procedure used for colony hybridization and why it is useful.

What type of vector can replicate and be maintained stably in two (or more)unrelated host organisms?

After digesting a DNA sequence,a restriction endonuclease can generate

Detecting a specific protein with an antibody is considered a(n)________ method.

The principle underlying how salmon were genetically engineered to grow faster is the

Which objective would be best to use a Southern blot rather than a Northern blot?

Which statement is TRUE?

The Ti plasmid is best suited for genetically manipulating

Using a host defective in proteases is likely to be necessary when engineering

The lacZ gene is commonly used as a reporter gene,because its substrate lactose is well known and easily measured.

To discover a catabolic gene cluster,cloning large sequences of approximately 40 kbp requires the utility of

The enzyme that covalently links both strands of a vector and inserted DNA in molecular cloning is