Question 1

RFLP's are the result of _____.

Accepted Answer

A) treating cells with enzymes that remove their cell walls 
B) fusing two cells together 
C) genes being transferred by a virus 
D) cutting DNA with restriction enzymes 
A) treating cells with enzymes that remove their cell walls 
B) fusing two cells together 
C) genes being transferred by a virus 
D) cutting DNA with restriction enzymes

Question 2

"Specialized" transduction refers to the fact that:

Accepted Answer

A) it only happens at specific times in the cell cycle 
B) only specific bacteria take part as hosts 
C) only a few specific genes are transferred 
D) only specific viruses take part in the process 
A) it only happens at specific times in the cell cycle 
B) only specific bacteria take part as hosts 
C) only a few specific genes are transferred 
D) only specific viruses take part in the process

Question 3

Without restriction endonucleases,it would be impossible to _____.

Accepted Answer

A) insert plasmids into bacteria 
B) bring about muations in bacteria 
C) replicate DNA in a recombinant cell 
D) cut the DNA of plasmids 
A) insert plasmids into bacteria 
B) bring about muations in bacteria 
C) replicate DNA in a recombinant cell 
D) cut the DNA of plasmids

Question 4

The transfer of DNA between bacterial cells by a virus is:

Accepted Answer

A) transformation 
B) transduction 
C) transferration 
D) conjugation 
A) transformation 
B) transduction 
C) transferration 
D) conjugation

Question 5

Competence refers to a bacterium's ability to _____.

Accepted Answer

A) synthesize a protein from another species gene that has been engineered 
B) undergo conjunction with an F⁺ cell 
C) take up DNA fragments 
D) be infected by a phage 
A) synthesize a protein from another species gene that has been engineered 
B) undergo conjunction with an F⁺ cell 
C) take up DNA fragments 
D) be infected by a phage

Question 6

DNA can be cut at specific sites by using _____.

Accepted Answer

A) ligases 
B) restriction enzymes 
C) antibiotics 
D) plasmids 
A) ligases 
B) restriction enzymes 
C) antibiotics 
D) plasmids

Question 7

Which of the following processes increases variation seen in bacteria?

Accepted Answer

A) Transformation 
B) Conjugation 
C) Transduction 
D) all of the above 
A) Transformation 
B) Conjugation 
C) Transduction 
D) all of the above

Question 8

Transposons are of particular significance because they _____.

Accepted Answer

A) come in pairs and often are associated with viruses 
B) regulate gene transcription in bacteria 
C) can be inserted using a gene gun 
D) can insert either between genes or in genes in both prokaryotes and eukaryotes 
A) come in pairs and often are associated with viruses 
B) regulate gene transcription in bacteria 
C) can be inserted using a gene gun 
D) can insert either between genes or in genes in both prokaryotes and eukaryotes

Question 9

Electroporation is a technique that is used to _____.

Accepted Answer

A) kill bacteria 
B) make bacteria competent 
C) increase conjugation among bacteria 
D) decrease the pathogenicity of bacteria 
A) kill bacteria 
B) make bacteria competent 
C) increase conjugation among bacteria 
D) decrease the pathogenicity of bacteria

Question 10

In 1981 a review of safety concerns concerning recombinant DNA research found that:

Accepted Answer

A) mutants of E. coli require special sanitization practices 
B) laboratory workers could become easily infected with the strain of E. coli normally used in lab experiments 
C) no illnesses could be traced to laboratory researchers working with recombinants 
D) two of the above are true 
A) mutants of E. coli require special sanitization practices 
B) laboratory workers could become easily infected with the strain of E. coli normally used in lab experiments 
C) no illnesses could be traced to laboratory researchers working with recombinants 
D) two of the above are true

Question 11

Gene therapy would describe which of the following scenarios?

Accepted Answer

A) Bacteria which produce human growth hormone 
B) A DNA comparison in a paternity case 
C) Yeast which has been genetically engineered to produce plastic from starch 
D) Replacing a defective gene in human cells 
A) Bacteria which produce human growth hormone 
B) A DNA comparison in a paternity case 
C) Yeast which has been genetically engineered to produce plastic from starch 
D) Replacing a defective gene in human cells

Question 12

A typical transposon is flanked by:

Accepted Answer

A) resistance factors 
B) inverted repeat terminals 
C) RTF genes 
D) repressor sites 
A) resistance factors 
B) inverted repeat terminals 
C) RTF genes 
D) repressor sites

Question 13

DNA from an organism can be incorporated into a vector using the enzyme _____.

Accepted Answer

A) DNA polymerase 
B) RNA polymerase 
C) DNA ligase 
D) restriction endonuclease 
A) DNA polymerase 
B) RNA polymerase 
C) DNA ligase 
D) restriction endonuclease

Question 14

A transgenic organism:

Accepted Answer

A) contains DNA from two different species of organisms 
B) contains a plasmid 
C) is resistant to viral infection 
D) contains DNA which allows it transfer plasmids from its cells to other cells 
A) contains DNA from two different species of organisms 
B) contains a plasmid 
C) is resistant to viral infection 
D) contains DNA which allows it transfer plasmids from its cells to other cells

Question 15

Which of the following may contribute to resistant strains of bacteria?

Accepted Answer

A) A 
B) B 
C) Both A and B are correct 
D) Neither A nor B are correct 
A) A 
B) B 
C) Both A and B are correct 
D) Neither A nor B are correct

Question 16

Bacteria that contain recombinant plasmids can be easily isolated because:

Accepted Answer

A) they are larger than nonrecombinant bacteria 
B) they are designed to be resistant to certain antibiotics 
C) their colonies will be distinguished by a color variation from those colonies containing nonrecombinant plasmids 
D) they are smaller than non-recombinant bacteria 
A) they are larger than nonrecombinant bacteria 
B) they are designed to be resistant to certain antibiotics 
C) their colonies will be distinguished by a color variation from those colonies containing nonrecombinant plasmids 
D) they are smaller than non-recombinant bacteria

Question 17

The Ti plasmid is known primarily for its ability to:

Accepted Answer

A) direct synthesis of conjugation pili 
B) provide resistance to certain antibiotics 
C) induce the formation of tumors in plants 
D) two of the above 
A) direct synthesis of conjugation pili 
B) provide resistance to certain antibiotics 
C) induce the formation of tumors in plants 
D) two of the above

Exam 8: Gene Transfer and Genetic Engineering

RFLP's are the result of _____.

"Specialized" transduction refers to the fact that:

Without restriction endonucleases,it would be impossible to _____.

The transfer of DNA between bacterial cells by a virus is:

Competence refers to a bacterium's ability to _____.

DNA can be cut at specific sites by using _____.

Which of the following processes increases variation seen in bacteria?

Transposons are of particular significance because they _____.

Electroporation is a technique that is used to _____.

In 1981 a review of safety concerns concerning recombinant DNA research found that:

Gene therapy would describe which of the following scenarios?

A typical transposon is flanked by:

DNA from an organism can be incorporated into a vector using the enzyme _____.

A transgenic organism:

Which of the following may contribute to resistant strains of bacteria?

Bacteria that contain recombinant plasmids can be easily isolated because:

The Ti plasmid is known primarily for its ability to:

Scope and History of Microbiology

Fundamentals of Chemistry

Microscopy and Staining

Characteristics of Prokaryotic and Eukaryotic Cells

Essential Concepts of Metabolism

Growth and Culturing of Bacteria

Microbial Genetics

An Introduction to Taxonomy: the Bacteria

Viruses

Eukaryotic Microorganisms and Parasites

Sterilization and Disinfection

Antimicrobial Therapy

Host-Microbe Relationships and Disease Processes

Epidemiology and Nosocomial Infections

Innate Host Defenses

Immunology I: Basic Principles of Adaptive Immunity and Immunization

Immunology Ii: Immunological Disorders and Tests

Diseases of the Skin and Eyes; Wounds and Bites

Urogenital and Sexually Transmitted Diseases

Diseases of the Respiratory System

Oral and Gastrointestinal Diseases

Cardiovascular, lymphatic and Systemic Disease

Diseases of the Nervous System

Environmental Microbiology

Applied Microbiology

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