Exam 8: Transport Across Membranes: Overcoming the Permeability Barrier

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Brucella abortus is a bacterium that utilizes an unusual sugar,erythritol.The concentration of erythritol within the B.abortus cell is 10 mM,while the external erythritol concentration is at very low levels,such as 0.20 mM.What is the energy requirement for the inward transport of erythritol at 37°C under these conditions?

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Begin with the equation: ΔG inward = + R T ln Begin with the equation: ΔG inward = + R T ln    As R = 1.987 cal/mol-K and T = K = °C + 273,the above equation is: ΔG inward = + (1.987)(37 + 273)ln = 615.97 ln 50 = 2409.69 cal/mol = +2.41 kcal/mol
As R = 1.987 cal/mol-K and T = K = °C + 273,the above equation is:
ΔG inward = + (1.987)(37 + 273)ln
= 615.97 ln 50 = 2409.69 cal/mol
= +2.41 kcal/mol

An example of indirect active transport is

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What is the ∆G′ for the inward transport of sucrose?

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A

When transporting charged molecules across a membrane,which of the following is/are true?

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Of the following molecules,which would not be expected to be moved across a membrane by simple diffusion?

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Choose the item that best matches the other items.
Na+/K+ pump
none of the listed processes
applies only to ions
diffusion
exclusively involves the transfer of small, nonpolar molecules
both diffusion and passive transport
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Responses:
Na+/K+ pump
none of the listed processes
applies only to ions
diffusion
exclusively involves the transfer of small, nonpolar molecules
both diffusion and passive transport
Keq = 1
active transport
ΔG° = 0
diffusion and passive and active transport
direction of transport is dependent upon the concentration gradient at any given point in time
passive transport
requires ligand binding and therefore Michaelis constants can be calculated
both passive and active transport
requires cellular expenditure of energy
indirect active transport
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What is the free energy change for the inward movement of calcium ions into the bacterial cell?

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In the kidney,the specialized transport proteins that allow for very rapid fluid movement into or out of cells are known as

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In all organisms,active transport across a membrane requires

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The Na⁺/K⁺ pump actively moves

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In some cells,it was noted that water moved faster than could be accounted for by simple diffusion.From this information,which of the following were identified and characterized?

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Mutations in bacterial porins can lead to antibiotic resistance typically by

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A(n)________ solution is one that contains a solute concentration that is ________ than that within the cell and results in the "bursting" of the cell.

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Which of the following is not an example of facilitated diffusion?

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Design an experiment whereby you would be able to distinguish between simple diffusion and facilitated diffusion of a substance.(Include graphs,if this aids in your discussion.)

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Transmembrane movement of a substance against a concentration gradient is called ________.

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The transport of two molecules across the cell membrane in the same direction is called ________.

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Which of the following is not a characteristic of active transport?

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Which of the following is/are true with regard to water movement in osmosis?

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Pores of the outer membrane of bacteria,mitochondria,and chloroplasts that are less specific and are formed by multipass transmembrane proteins are called ________.

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