Question 1

Which would be best to separate positively charged proteins?

Accepted Answer

A)  Gel filtration 
B)  Affinity chromatography 
C)  Cation exchange 
D)  Anion exchange 
E)  Cation or anion exchange 
A)  Gel filtration 
B)  Affinity chromatography 
C)  Cation exchange 
D)  Anion exchange 
E)  Cation or anion exchange

Question 2

In an ELISA, the compound 4-chloro-1-naphthol is used because

Accepted Answer

A)  it helps the secondary antibody to bind to the protein 
B)  it helps the primary antibody bind to the protein 
C)  it turns color in the presence of an enzyme that is bound to the secondary antibody 
D)  all of the choices 
A)  it helps the secondary antibody to bind to the protein 
B)  it helps the primary antibody bind to the protein 
C)  it turns color in the presence of an enzyme that is bound to the secondary antibody 
D)  all of the choices

Question 3

Studying the entire complement of proteins being produced by a cell is called

Accepted Answer

A)  genomics 
B)  proteomics 
C)  ELISA 
D)  western blot 
A)  genomics 
B)  proteomics 
C)  ELISA 
D)  western blot

Question 4

A separation of a mixture of cations of different charge requires

Accepted Answer

A)  another cationic substance 
B)  an anionic substance 
C)  an electrically neutral, but highly polar, substance 
D)  an electrically neutral, nonpolar substance 
A)  another cationic substance 
B)  an anionic substance 
C)  an electrically neutral, but highly polar, substance 
D)  an electrically neutral, nonpolar substance

Question 5

The following methods are useful for cell homogenization:

Accepted Answer

A)  Sonication. 
B)  Freezing and thawing. 
C)  Detergents. 
D)  Enzymes. 
E)  All of these are correct. 
A)  Sonication. 
B)  Freezing and thawing. 
C)  Detergents. 
D)  Enzymes. 
E)  All of these are correct.

Question 6

The purity of an enzyme at various stages of purification is best measured by

Accepted Answer

A)  total protein. 
B)  total enzyme activity. 
C)  specific activity of the enzyme. 
D)  percent recovery of the protein. 
E)  percent recovery of the enzyme. 
A)  total protein. 
B)  total enzyme activity. 
C)  specific activity of the enzyme. 
D)  percent recovery of the protein. 
E)  percent recovery of the enzyme.

Question 7

Cyanogen bromide (CNBr) cleaves proteins

Accepted Answer

A)  after positively charged residues, such as K & R. 
B)  after negatively charged residues, such as D & E. 
C)  after aromatic residues, such as Y & W. 
D)  after methionine residues. 
A)  after positively charged residues, such as K & R. 
B)  after negatively charged residues, such as D & E. 
C)  after aromatic residues, such as Y & W. 
D)  after methionine residues.

Which would be best to separate positively charged proteins?

In an ELISA, the compound 4-chloro-1-naphthol is used because

Studying the entire complement of proteins being produced by a cell is called

A separation of a mixture of cations of different charge requires

The following methods are useful for cell homogenization:

The purity of an enzyme at various stages of purification is best measured by

Cyanogen bromide (CNBr) cleaves proteins

Biochemistry and the Organization of Cells

Water: the Solvent for Biochemical Reactions

Amino Acids and Peptides

The Three-Dimensional Structure of Proteins

The Behavior of Proteins: Enzymes

The Behavior of Proteins: Enzymes, Mechanisms, and Control

Lipids and Proteins Are Associated in Biological Membranes

Nucleic Acids: How Structure Conveys Information

Biosynthesis of Nucleic Acids: Replication

Transcription of the Genetic Code: Biosynthesis of RNA

Protein Synthesis: Translation of the Genetic Message

Nucleic Acid Biotechnology Techniques

Viruses, Cancer, and Immunology

The Importance of Energy Changes and Electron Transfer in Metabolism

Carbohydrates

Glycolysis

Storage Mechanisms and Control in Carbohydrate Metabolism

The Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Lipid Metabolism

Photosynthesis

The Metabolism of Nitrogen

Integration of Metabolism: Cellular Signaling

Filters

Exam 5: Protein Purification and Characterization Techniques

Which would be best to separate positively charged proteins?

In an ELISA, the compound 4-chloro-1-naphthol is used because

Studying the entire complement of proteins being produced by a cell is called

A separation of a mixture of cations of different charge requires

The following methods are useful for cell homogenization:

The purity of an enzyme at various stages of purification is best measured by

Cyanogen bromide (CNBr) cleaves proteins

Biochemistry and the Organization of Cells

Water: the Solvent for Biochemical Reactions

Amino Acids and Peptides

The Three-Dimensional Structure of Proteins

The Behavior of Proteins: Enzymes

The Behavior of Proteins: Enzymes, Mechanisms, and Control

Lipids and Proteins Are Associated in Biological Membranes

Nucleic Acids: How Structure Conveys Information

Biosynthesis of Nucleic Acids: Replication

Transcription of the Genetic Code: Biosynthesis of RNA

Protein Synthesis: Translation of the Genetic Message

Nucleic Acid Biotechnology Techniques

Viruses, Cancer, and Immunology

The Importance of Energy Changes and Electron Transfer in Metabolism

Carbohydrates

Glycolysis

Storage Mechanisms and Control in Carbohydrate Metabolism

The Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Lipid Metabolism

Photosynthesis

The Metabolism of Nitrogen

Integration of Metabolism: Cellular Signaling

Filters