Exam 5: Protein Purification and Characterization Techniques

Exhibit 5A The following diagram shows the anode, cathode, and $\mathrm { pH }$ gradient of an isoelectric focusing bed: -Refer to Exhibit 5A. If the amino acid glycine were placed in the bed where the pH is 7, and the current were turned on, it would migrate closest to which of the following positions?

In affinity chromatography, a protein

The most common way of visualizing the results seen on a protein chip is by use of

Exhibit 5A The following diagram shows the anode, cathode, and $\mathrm { pH }$ gradient of an isoelectric focusing bed: -Refer to Exhibit 5A. A mixture of asp, asn, and arg is placed in the bed where the pH is 7, and the current is turned on. From left to right, which best represents the final positions of the individual amino acids?

If a protein with the sequence PQRKYPIG is treated with trypsin, what will the products be?

Which of the following is not used in protein structure determination?

The following are all principles on which to base electrophoresis except:

In any form of chromatography, how will a compound which interacts more strongly with the stationary phase elute compared to one that interacts less strongly?

Two dimensional separation methods

The degree of separation in molecular sieve chromatography depends on

Which type of column is most affected by the shape of the protein, for example, comparing spherical and cigar-shaped proteins?

What tends to happen to the percent recovery during a protein's purification?

Determination of the sequence of amino acids in a peptide is done by

In gel filtration chromatography

Which would be best to separate proteins of similar size?

The purpose of using nitrocellulose for a western blot is to

A protein chip is functionally most similar to which other technique?

Which of the following explains why a secondary antibody is used in an ELISA?

In a sample consisting of lysine, leucine, and glutamic acid, which will be eluted last from an anion exchange resin at pH 7?

In an ELISA, the molecule that binds directly to the target protein is called the