Question 1

A fragment of DNA or RNA labeled with a tracer that can hybridize with a nucleotide sequence of interest is referred to as a ____.

Accepted Answer

A) clone 
B) plasmid 
C) vector 
D) marker 
E) probe 
A) clone 
B) plasmid 
C) vector 
D) marker 
E) probe

Question 2

Which process consists of modifying an individual's genome?

Accepted Answer

A) DNA hybridization 
B) PCR 
C) SNPing 
D) DNA profiling 
E) genetic engineering 
A) DNA hybridization 
B) PCR 
C) SNPing 
D) DNA profiling 
E) genetic engineering

Question 3

What is the most assured method of identifying an individual?

Accepted Answer

A) full genome sequencing 
B) short tandem repeat analysis 
C) SNP-chip analysis 
D) DNA-DNA hybridization 
E) PCR amplification 
A) full genome sequencing 
B) short tandem repeat analysis 
C) SNP-chip analysis 
D) DNA-DNA hybridization 
E) PCR amplification

Question 4

During a DNA profiling analysis, short tandem repeats are first ____.

Accepted Answer

A) subjected to high heat 
B) inserted into a plasmid 
C) chopped up using a restriction enzyme 
D) placed on a gel for electrophoresis 
E) amplified using PCR 
A) subjected to high heat 
B) inserted into a plasmid 
C) chopped up using a restriction enzyme 
D) placed on a gel for electrophoresis 
E) amplified using PCR

Question 5

In bacteria, restriction enzymes function to ____.

Accepted Answer

A) identify sequences of interest in a DNA library 
B) produce human proteins 
C) provide antibiotic resistance 
D) chop up invading viral DNA 
E) make the cells vulnerable to viruses 
A) identify sequences of interest in a DNA library 
B) produce human proteins 
C) provide antibiotic resistance 
D) chop up invading viral DNA 
E) make the cells vulnerable to viruses

Question 6

Which special property of Taq polymerases make them useful for PCR?

Accepted Answer

A) they are stable at high temperatures 
B) their hydrogen bonds do not break at low pHs 
C) they multiply quickly 
D) they are easy to isolate from common fungi 
E) they paste two DNA fragments together 
A) they are stable at high temperatures 
B) their hydrogen bonds do not break at low pHs 
C) they multiply quickly 
D) they are easy to isolate from common fungi 
E) they paste two DNA fragments together

Question 7

Match between columns
                        Premises: The number of copies of the targeted DNA section is doubled with each cycle of heating and cooling. After two rounds, the amount of the initial DNA template has doubled twice.

Accepted Answer

The Answer of D and E and A and C is...

Question 8

Once a probe has hybridized with a gene of interest in a DNA library, the hybrid is identified by detection of ____.

Accepted Answer

A) its sticky tail 
B) its radioactive label 
C) restriction enzymes 
D) mRNA 
E) the vector 
A) its sticky tail 
B) its radioactive label 
C) restriction enzymes 
D) mRNA 
E) the vector

Question 9

When performing gene therapy, DNA can be introduced into human cells by using genetically engineered ____, which are designed to deliver their genetic material into the cells they infect.

Accepted Answer

A) sheep cells 
B) mouse cells 
C) bacteria 
D) plasmids 
E) viruses 
A) sheep cells 
B) mouse cells 
C) bacteria 
D) plasmids 
E) viruses

Question 10

Match between columns
                        Premises: in a bacterial population, each individual contains several copies of the same recombinant DNA molecule

Accepted Answer

The Answer of recombinant DNA and plasmids and clones and is...

Question 11

Sticky ends are so named because ____.

Accepted Answer

A) they are single stranded and will base pair with complementary  sequences 
B) covalent bonds spontaneously form between them to form a continuous DNA molecule 
C) primers hybridize to them during the first step of PCR 
D) they hybridize to a DNA sequence of interest 
E) they stick to invading viral DNA 
A) they are single stranded and will base pair with complementary  sequences 
B) covalent bonds spontaneously form between them to form a continuous DNA molecule 
C) primers hybridize to them during the first step of PCR 
D) they hybridize to a DNA sequence of interest 
E) they stick to invading viral DNA

Question 12

Beta-carotene is converted to vitamin ____ in the cells of the small intestine.

Accepted Answer

A) B12 
B) ​A 
C) ​E 
D) ​C 
E) D​ 
A) B12 
B) ​A 
C) ​E 
D) ​C 
E) D​

Question 13

How much Golden Rice will supply a child's daily need for the vitamin that is converted from beta-carotene?

Accepted Answer

A) two teaspoons 
B) a half of a cup 
C) one cup 
D) one quart 
E) one pint 
A) two teaspoons 
B) a half of a cup 
C) one cup 
D) one quart 
E) one pint

Question 14

Which of the following happens first in the PCR process?

Accepted Answer

A) repeated cycles of high and low temperatures 
B) mixing of DNA polymerase with template DNA, nucleotides, and primers 
C) DNA hybridization 
D) DNA synthesis 
E) DNA unwinds 
A) repeated cycles of high and low temperatures 
B) mixing of DNA polymerase with template DNA, nucleotides, and primers 
C) DNA hybridization 
D) DNA synthesis 
E) DNA unwinds

Question 15

Match between columns
                        Premises: A DNA molecule that can accept foreign DNA and be replicated inside a host cell.

Accepted Answer

The Answer of single-nucleotide polymorphism and cloning vector and probe is...

Question 16

Currently, the only possible cure for genetic disorders is ____.

Accepted Answer

A) genetically modified drugs 
B) transplantation 
C) whole genome replacement 
D) gene therapy 
E) GMOs 
A) genetically modified drugs 
B) transplantation 
C) whole genome replacement 
D) gene therapy 
E) GMOs

Question 17

The technique of ____ analyzes 500,000 SNPs in an individual's genome.

Accepted Answer

A) electrophoresis 
B) cutting and pasting 
C) DNA cloning 
D) PCR 
E) SNP-chips 
A) electrophoresis 
B) cutting and pasting 
C) DNA cloning 
D) PCR 
E) SNP-chips

Question 18

Match between columns
                        Premises: technique of separating DNA fragments of different sizes

Accepted Answer

The Answer of genetic engineering and DNA profile and DNA is...

Question 19

When DNA fragments are inserted into plasmids, the resulting recombinant molecules must be introduced into ____ in order for the DNA to be replicated.

Accepted Answer

A) rabbits 
B) humans 
C) bacteria 
D) mice 
E) chimpanzees 
A) rabbits 
B) humans 
C) bacteria 
D) mice 
E) chimpanzees

Question 20

What do biologists use in order to identify clones that contain the DNA fragment of interest?

Accepted Answer

A) probes 
B) plasmids 
C) templates 
D) primers 
E) ligases 
A) probes 
B) plasmids 
C) templates 
D) primers 
E) ligases

Exam 10: Biotechnology

A fragment of DNA or RNA labeled with a tracer that can hybridize with a nucleotide sequence of interest is referred to as a ____.

Which process consists of modifying an individual's genome?

What is the most assured method of identifying an individual?

During a DNA profiling analysis, short tandem repeats are first ____.

In bacteria, restriction enzymes function to ____.

Which special property of Taq polymerases make them useful for PCR?

Once a probe has hybridized with a gene of interest in a DNA library, the hybrid is identified by detection of ____.

When performing gene therapy, DNA can be introduced into human cells by using genetically engineered ____, which are designed to deliver their genetic material into the cells they infect.

Sticky ends are so named because ____.

Beta-carotene is converted to vitamin ____ in the cells of the small intestine.

How much Golden Rice will supply a child's daily need for the vitamin that is converted from beta-carotene?

Which of the following happens first in the PCR process?

Currently, the only possible cure for genetic disorders is ____.

The technique of ____ analyzes 500,000 SNPs in an individual's genome.

When DNA fragments are inserted into plasmids, the resulting recombinant molecules must be introduced into ____ in order for the DNA to be replicated.

What do biologists use in order to identify clones that contain the DNA fragment of interest?

Invitation to Biology

Molecules of Life

Cell Structure

Energy and Metabolism

Capturing and Releasing Energy

Dna Structure and Function

Gene Expression and Control

How Cells Reproduce

Patterns of Inheritance

Evidence of Evolution

Processes of Evolution

Early Life Forms and the Viruses

Plants and Fungi

Animal Evolution

Population Ecology

Communities and Ecosystems

The Biosphere and Human Effects

Animal Tissues and Organs

How Animals Move

Circulation and Respiration

Immunity

Digestion and Excretion

Neural Control and the Senses

Endocrine Control

Reproduction and Development

Plant Form and Function

Plant Reproduction and Development

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