Question 1

Tissue specimens require disaggregation before analysis by flow cytometry.

Accepted Answer

A) True 
 B)False  True

Question 2

The major application(s) of flow cell technology is (are)

Accepted Answer

A) identification of cells. 
B) cell sorting before further analysis. 
C) diagnosis of autoimmune disease. 
D) Both A and B. 
A) identification of cells. 
B) cell sorting before further analysis. 
C) diagnosis of autoimmune disease. 
D) Both A and B. D

Question 3

Laser light is multicolored.

Accepted Answer

A) True 
 B)False  False

Question 4

Immunophenotyping includes the detection or identification of

Accepted Answer

A) CD34+ stem cells. 
B) minimal residual disease in patients with leukemia. 
C) T and B lymphocytes. 
D) All of the above. 
A) CD34+ stem cells. 
B) minimal residual disease in patients with leukemia. 
C) T and B lymphocytes. 
D) All of the above.

Question 5

The most common type of laser light used in laboratory instrumentation is neon.

Accepted Answer

A) True 
 B)False

Question 6

Laser is an acronym for

Accepted Answer

A) light amplification by stimulated emission of radiation. 
B) light augmentation by stimulated emitted radiation. 
C) light amplified by stimulated energy radiation. 
D) large angle stimulation by emitted radiation. 
A) light amplification by stimulated emission of radiation. 
B) light augmentation by stimulated emitted radiation. 
C) light amplified by stimulated energy radiation. 
D) large angle stimulation by emitted radiation.

Question 7

Match the activity with the appropriate phase of testing related to laboratory automation.
-Specimen labeling

Accepted Answer

A) Preanalytical 
B) Analytical 
C) Postanalytical 
A) Preanalytical 
B) Analytical 
C) Postanalytical

Question 8

The principle of flow cytometry is based on

Accepted Answer

A) antigen-antibody reactions. 
B) the formation of immune complexes. 
C) the reaction of dyes with the cellular component of interest. 
D) the reaction of dyes with immune complexes. 
A) antigen-antibody reactions. 
B) the formation of immune complexes. 
C) the reaction of dyes with the cellular component of interest. 
D) the reaction of dyes with immune complexes.

Question 9

A fluorochrome is a(n)

Accepted Answer

A) dye. 
B) immunologic reagent. 
C) nonspecific indicator. 
D) Both A and B. 
A) dye. 
B) immunologic reagent. 
C) nonspecific indicator. 
D) Both A and B.

Question 10

Match the activity with the appropriate phase of testing related to laboratory automation.
-Documentation of quality control data

Accepted Answer

A) Preanalytical 
B) Analytical 
C) Postanalytical 
A) Preanalytical 
B) Analytical 
C) Postanalytical

Question 11

Match the activity with the appropriate phase of testing related to laboratory automation.
-Validation of results

Accepted Answer

A) Preanalytical 
B) Analytical 
C) Postanalytical 
A) Preanalytical 
B) Analytical 
C) Postanalytical

Question 12

Blood specimens for flow cytometry analysis should be refrigerated.

Accepted Answer

A) True 
 B)False

Question 13

A photon is a

Accepted Answer

A) basic unit of light. 
B) basic unit of all radiation. 
C) component of an atom. 
D) component of laser light. 
A) basic unit of light. 
B) basic unit of all radiation. 
C) component of an atom. 
D) component of laser light.

Question 14

The most frequently used fluorochrome(s) is (are)

Accepted Answer

A) fluorescein isothiocyanate (FIT
C) . 
B) phycoerythrin (P
E) . 
C) peridinin chlorophyll protein (PerCP). 
D) All of the above. 
A) fluorescein isothiocyanate (FIT
C) . 
B) phycoerythrin (P
E) . 
C) peridinin chlorophyll protein (PerCP). 
D) All of the above.

Question 15

Lasers sort the energy in atoms and molecules, concentrate it, and release it in powerful waves.

Accepted Answer

A) True 
 B)False

Question 16

Match the activity with the appropriate phase of testing related to laboratory automation.
-Quality control

Accepted Answer

A) Preanalytical 
B) Analytical 
C) Postanalytical 
A) Preanalytical 
B) Analytical 
C) Postanalytical

Question 17

Current fluorescent methods can perform up to _______-color analysis.

Accepted Answer

A) two 
B) four 
C) eight 
D) sixteen 
A) two 
B) four 
C) eight 
D) sixteen

Question 18

Laser light is concentrated.

Accepted Answer

A) True 
 B)False

Exam 13: Flow Cytometry

Tissue specimens require disaggregation before analysis by flow cytometry.

The major application(s) of flow cell technology is (are)

Laser light is multicolored.

Immunophenotyping includes the detection or identification of

The most common type of laser light used in laboratory instrumentation is neon.

Laser is an acronym for

Match the activity with the appropriate phase of testing related to laboratory automation. -Specimen labeling

The principle of flow cytometry is based on

A fluorochrome is a(n)

Match the activity with the appropriate phase of testing related to laboratory automation. -Documentation of quality control data

Match the activity with the appropriate phase of testing related to laboratory automation. -Validation of results

Blood specimens for flow cytometry analysis should be refrigerated.

A photon is a

The most frequently used fluorochrome(s) is (are)

Lasers sort the energy in atoms and molecules, concentrate it, and release it in powerful waves.

Match the activity with the appropriate phase of testing related to laboratory automation. -Quality control

Current fluorescent methods can perform up to _______-color analysis.

Laser light is concentrated.

An Overview of Immunology

Antigens and Antibodies

Cells and Cellular Activities of the Immune System: Granulocytes and Mononuclear Cells

Cells and Cellular Activities of the Immune System: Lymphocytes and Plasma Cells

Soluble Mediators of the Immune System

Safety in the Immunology-Serology Laboratory

Quality Assurance and Quality Control

Basic Serologic Laboratory Techniques

Rapid Testing

Precipitation and Particle Agglutination Methods

Electrophoresis Techniques

Labeling Techniques in Immunoassay

Molecular Laboratory Techniques

Infectious Diseases: Traditional and Alternate Diagnostic Techniques

Streptococcal Infections

Syphilis

Vector-Borne Diseases

Toxoplasmosis

Cytomegalovirus

Infectious Mononucleosis

Viral Hepatitis

Rubella and Rubeola Infections

Primary and Acquired Immune Deficiency Syndromes

Hypersensitivity Reactions

Immunoproliferative Disorders

Tolerance, Autoimmunity, and Autoimmune Diseases

Systemic Lupus Erythematosus

Rheumatoid Arthritis

Transplantation: Hla, Solid Organ, and Hematopoietic Stem Cells

Tumor Immunology and Up-To-Date Applications of Next-Generation Sequencing

Primer on Vaccines

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